Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/122599 |
Resumo: | Funding Information: This research was funded by Global Health and Tropical Medicine: Grant number IHMT-UID/multi/04413/2013 and Grant number PTDC/CVT-CVT/28908/2017, FCT-Portugal. Funding Information: We would like to thank Prof. João Aristeu da Rosa and Dr. Aline Rimodi Rimeiro at UNESP (Universidade Estadual de São Paulo) Araraquara (Brazil) for offering four different strains of T. cruzi. JWFO, CJGM, and BAC thanks to the financial support (PhD and Post-doctoral fellowships) provided by Capes/Brazil; MSS and HAOR thanks to CNPq/Brazil for the Research Grant (Bolsa de Produtividade). We also would like to thank the Department of Materials Engineering at UFRN for allowing the use of their scanning electron microscope, and the Department of Biochemistry at UFRN for allowing the use of their Flow Cytometer. We are also grateful to Paulo Fanado for editing this manuscript. Publisher Copyright: © 2021, The Author(s). |
| id |
RCAP_a8711c2f79c177c1a957446acb7cc47b |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/122599 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruziParasitologyDrug DiscoverySDG 3 - Good Health and Well-beingFunding Information: This research was funded by Global Health and Tropical Medicine: Grant number IHMT-UID/multi/04413/2013 and Grant number PTDC/CVT-CVT/28908/2017, FCT-Portugal. Funding Information: We would like to thank Prof. João Aristeu da Rosa and Dr. Aline Rimodi Rimeiro at UNESP (Universidade Estadual de São Paulo) Araraquara (Brazil) for offering four different strains of T. cruzi. JWFO, CJGM, and BAC thanks to the financial support (PhD and Post-doctoral fellowships) provided by Capes/Brazil; MSS and HAOR thanks to CNPq/Brazil for the Research Grant (Bolsa de Produtividade). We also would like to thank the Department of Materials Engineering at UFRN for allowing the use of their scanning electron microscope, and the Department of Biochemistry at UFRN for allowing the use of their Flow Cytometer. We are also grateful to Paulo Fanado for editing this manuscript. Publisher Copyright: © 2021, The Author(s).Chagas disease is caused by Trypanosoma cruzi and affects thousands of people. Drugs currently used in therapy are toxic and have therapeutic limitations. In addition, the genetic diversity of T. cruzi represents an important variable and challenge in treatment. Sodium diethyldithiocarbamate (DETC) is a compound with pharmacological versatility acting as metal chelators and ROS generation. Thus, the objective was to characterize the antiparasitic action of DETC against different strains and forms of T. cruzi and their mechanism. The different strains of T. cruzi were grown in LIT medium. To evaluate the antiparasitic activity of DETC, epimastigote and trypomastigote forms of T. cruzi were used by resazurin reduction methods and by counting. Different response patterns were obtained between the strains and an IC50 of DETC ranging from 9.44 ± 3,181 to 60.49 ± 7.62 µM. Cell cytotoxicity against 3T3 and RAW cell lines and evaluated by MTT, demonstrated that DETC in high concentration (2222.00 µM) presents low toxicity. Yet, DETC causes mitochondrial damage in T. cruzi, as well as disruption in parasite membrane. DETC has antiparasitic activity against different genotypes and forms of T. cruzi, therefore, representing a promising molecule as a drug for the treatment of Chagas disease.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNde Freitas Oliveira, Johny WysllasTorres, Taffarel MeloMoreno, Cláudia Jassica GonçalvesAmorim-Carmo, BrunoDamasceno, Igor ZumbaSoares, Ana Katarina Menezes Cruzda Silva Barbosa, JeffersonRocha, Hugo Alexandre OliveiraSilva, Marcelo Sousa2021-08-16T23:20:26Z2021-122021-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/122599eng2045-2322PURE: 33050263https://doi.org/10.1038/s41598-021-90719-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:04:10Zoai:run.unl.pt:10362/122599Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:47.988612Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| title |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| spellingShingle |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi de Freitas Oliveira, Johny Wysllas Parasitology Drug Discovery SDG 3 - Good Health and Well-being |
| title_short |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| title_full |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| title_fullStr |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| title_full_unstemmed |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| title_sort |
Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi |
| author |
de Freitas Oliveira, Johny Wysllas |
| author_facet |
de Freitas Oliveira, Johny Wysllas Torres, Taffarel Melo Moreno, Cláudia Jassica Gonçalves Amorim-Carmo, Bruno Damasceno, Igor Zumba Soares, Ana Katarina Menezes Cruz da Silva Barbosa, Jefferson Rocha, Hugo Alexandre Oliveira Silva, Marcelo Sousa |
| author_role |
author |
| author2 |
Torres, Taffarel Melo Moreno, Cláudia Jassica Gonçalves Amorim-Carmo, Bruno Damasceno, Igor Zumba Soares, Ana Katarina Menezes Cruz da Silva Barbosa, Jefferson Rocha, Hugo Alexandre Oliveira Silva, Marcelo Sousa |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) RUN |
| dc.contributor.author.fl_str_mv |
de Freitas Oliveira, Johny Wysllas Torres, Taffarel Melo Moreno, Cláudia Jassica Gonçalves Amorim-Carmo, Bruno Damasceno, Igor Zumba Soares, Ana Katarina Menezes Cruz da Silva Barbosa, Jefferson Rocha, Hugo Alexandre Oliveira Silva, Marcelo Sousa |
| dc.subject.por.fl_str_mv |
Parasitology Drug Discovery SDG 3 - Good Health and Well-being |
| topic |
Parasitology Drug Discovery SDG 3 - Good Health and Well-being |
| description |
Funding Information: This research was funded by Global Health and Tropical Medicine: Grant number IHMT-UID/multi/04413/2013 and Grant number PTDC/CVT-CVT/28908/2017, FCT-Portugal. Funding Information: We would like to thank Prof. João Aristeu da Rosa and Dr. Aline Rimodi Rimeiro at UNESP (Universidade Estadual de São Paulo) Araraquara (Brazil) for offering four different strains of T. cruzi. JWFO, CJGM, and BAC thanks to the financial support (PhD and Post-doctoral fellowships) provided by Capes/Brazil; MSS and HAOR thanks to CNPq/Brazil for the Research Grant (Bolsa de Produtividade). We also would like to thank the Department of Materials Engineering at UFRN for allowing the use of their scanning electron microscope, and the Department of Biochemistry at UFRN for allowing the use of their Flow Cytometer. We are also grateful to Paulo Fanado for editing this manuscript. Publisher Copyright: © 2021, The Author(s). |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-08-16T23:20:26Z 2021-12 2021-12-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/122599 |
| url |
http://hdl.handle.net/10362/122599 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2045-2322 PURE: 33050263 https://doi.org/10.1038/s41598-021-90719-0 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138054723600384 |