Technologies for high-throughput identification of antibiotic mechanism of action

Detalhes bibliográficos
Autor(a) principal: Ribeiro Da Cunha, Bernardo
Data de Publicação: 2021
Outros Autores: Zoio, Paulo, Fonseca, Luís P. P., Calado, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/13416
Resumo: Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
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spelling Technologies for high-throughput identification of antibiotic mechanism of actionAntibiotic discoveryBacterial cytological profilingChemical geneticsHigh-throughput screeningMechanism-of-Action (MOA)MetabolomicsPhenotypic screeningProteomicsTranscriptomicsVibrational spectroscopyEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISELEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2020 do Instituto Politécnico de Lisboa. Código de referência IPL/2020/NephroMD/ISELThere are two main strategies for antibiotic discovery: target-based and phenotypic screening. The latter has been much more successful in delivering first-in-class antibiotics, despite the major bottleneck of delayed Mechanism-of-Action (MOA) identification. Although finding new antimicrobial compounds is a very challenging task, identifying their MOA has proven equally challenging. MOA identification is important because it is a great facilitator of lead optimization and improves the chances of commercialization. Moreover, the ability to rapidly detect MOA could enable a shift from an activity-based discovery paradigm towards a mechanism-based approach. This would allow to probe the grey chemical matter, an underexplored source of structural novelty. In this study we review techniques with throughput suitable to screen large libraries and sufficient sensitivity to distinguish MOA. In particular, the techniques used in chemical genetics (e.g., based on overexpression and knockout/knockdown collections), promoter-reporter libraries, transcriptomics (e.g., using microarrays and RNA sequencing), proteomics (e.g., either gel-based or gel-free techniques), metabolomics (e.g., resourcing to nuclear magnetic resonance or mass spectrometry techniques), bacterial cytological profiling, and vibrational spectroscopy (e.g., Fourier-transform infrared or Raman scattering spectroscopy) were discussed. Ultimately, new and reinvigorated phenotypic assays bring renewed hope in the discovery of a new generation of antibiotics.MDPIRCIPLRibeiro Da Cunha, BernardoZoio, PauloFonseca, Luís P. P.Calado, Cecília2021-06-04T10:12:52Z2021-05-122021-05-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/13416engCUNHA, Bernardo Ribeiro da; [et al] – Technologies for high-throughput identification of antibiotic mechanism of action. Antibiotics. ISSN 2079-6382. Vol. 10, N.º 5 (2021), pp. 1-202079-638210.3390/antibiotics10050565info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:08:05Zoai:repositorio.ipl.pt:10400.21/13416Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:21:22.168932Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Technologies for high-throughput identification of antibiotic mechanism of action
title Technologies for high-throughput identification of antibiotic mechanism of action
spellingShingle Technologies for high-throughput identification of antibiotic mechanism of action
Ribeiro Da Cunha, Bernardo
Antibiotic discovery
Bacterial cytological profiling
Chemical genetics
High-throughput screening
Mechanism-of-Action (MOA)
Metabolomics
Phenotypic screening
Proteomics
Transcriptomics
Vibrational spectroscopy
title_short Technologies for high-throughput identification of antibiotic mechanism of action
title_full Technologies for high-throughput identification of antibiotic mechanism of action
title_fullStr Technologies for high-throughput identification of antibiotic mechanism of action
title_full_unstemmed Technologies for high-throughput identification of antibiotic mechanism of action
title_sort Technologies for high-throughput identification of antibiotic mechanism of action
author Ribeiro Da Cunha, Bernardo
author_facet Ribeiro Da Cunha, Bernardo
Zoio, Paulo
Fonseca, Luís P. P.
Calado, Cecília
author_role author
author2 Zoio, Paulo
Fonseca, Luís P. P.
Calado, Cecília
author2_role author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Ribeiro Da Cunha, Bernardo
Zoio, Paulo
Fonseca, Luís P. P.
Calado, Cecília
dc.subject.por.fl_str_mv Antibiotic discovery
Bacterial cytological profiling
Chemical genetics
High-throughput screening
Mechanism-of-Action (MOA)
Metabolomics
Phenotypic screening
Proteomics
Transcriptomics
Vibrational spectroscopy
topic Antibiotic discovery
Bacterial cytological profiling
Chemical genetics
High-throughput screening
Mechanism-of-Action (MOA)
Metabolomics
Phenotypic screening
Proteomics
Transcriptomics
Vibrational spectroscopy
description Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
publishDate 2021
dc.date.none.fl_str_mv 2021-06-04T10:12:52Z
2021-05-12
2021-05-12T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/13416
url http://hdl.handle.net/10400.21/13416
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv CUNHA, Bernardo Ribeiro da; [et al] – Technologies for high-throughput identification of antibiotic mechanism of action. Antibiotics. ISSN 2079-6382. Vol. 10, N.º 5 (2021), pp. 1-20
2079-6382
10.3390/antibiotics10050565
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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