Validation of fast-read microscopic slide on urine sediment examination of dogs and cats – a preliminary study

• Autor(a) principal: Parra, Wendy Silva
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10348/10252
• topic: urinalysis; sediment

Urinalysis is a diagnostic screening test that can diagnose and monitor genitourinary, kidney and metabolic diseases even before the onset of clinical signs. Although it is an extremely useful, non-invasive, readily available and inexpensive tool it is a much-neglected test in veterinary medicine because it’s ponderous, time-consuming, imprecise and has wide variability. This study evaluated the diagnostic accuracy and interchangeable capacity of the counting cell chamber Pentasquare slide in comparison with the traditional method of urine sediment using slide and coverslip. This study included 34 patients, 17 dogs and 17 cats, that were admitted in the veterinary hospital of the University of Trás-os-Montes e Alto Douro (HV-UTAD) between May 2nd and July 31st of 2019. The population under study had urinalysis performed whether because a genitourinary or kidney disease was suspected or to evaluate the evolution of a previous diagnosed pathology. To every patient a type II urinalysis was performed (dipsticks and urine sediment). In all cases sediment evaluation was made with 3 preparations: one traditional method for urine sediment preparation and two preparations on the counting cell chambers in study. The statistical analysis of method comparison by using Passing-Bablock regression and Bland Altman plot demonstrated that the counting cell chambers can be used interchangeably with the slide and coverslip method. For qualitative parameters the sensitivity and specificity revealed high values, between 43% to 84% and 89% to 100% respectively. The agreement rate between methods was moderate to substantial (values from 0,47 to 0,77), assessed by the calculated Cohen’s Kappa value. Besides these values that assess the capacity of the counting cell chamber to replace the traditional method, reproducibility and repeatability were also calculated. Repeatability revealed high values of correlation between observers, being the lowest value obtained of 68% and the highest of 100%. As for reproducibility the counting cell chambers revealed correlation values of 61% to 100% for different elements, except for calcium oxalate crystals. Even though more studies are needed to evaluate the use of the Pentasquare slide as reference method for urine sediment in veterinary medicine this preliminary study verifies that counting cell chambers can be used for urine sediment evaluation in dogs and cats.