Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctane reversed micelles

• Autor(a) principal: Nascimento, Cynthia O.
• Data de Publicação: 2008
• Outros Autores: Costa, Romero M. P. B., Araújo, Regina M. S., Chaves, Maria E. C., Coelho, L. C. B. B., Paiva, Patrícia M. G., Teixeira, J. A., Correia, M. T. S., Carneiro-da-Cunha, Maria G.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://hdl.handle.net/1822/7978
• topic: Liquid–liquid extraction; Reversed micelles; Sodium di(2-ethylhexyl)sulfosuccinate; (AOT); Purification; Lectin; Crataeva tapia; Sodium di(2-ethylhexyl)sulfosuccinate (AOT); Science & Technology

Crataeva tapia bark lectin was extracted from a crude extract into a reversed micelle phase of the anionic surfactant AOT in isooctane and back-extracted, to a final aqueous phase by addition of butanol. The effects of pH, ionic strength and surfactant concentration on the protein transfer process from the aqueous to the organic phase were characterized, being the best results obtained after 5 min of contact, under agitation, between the two phases, at pH 5.5 (10 mM citrate-phosphate buffer), 30 mM NaCl, and 5 mM AOT. Recovery to a new aqueous phase was performed with 5 min of contact, under agitation, 10 mM citrate-phosphate buffer at pH 5.5, 500 mM KCl and 5% of butanol. The overall yield obtained for the process was 80% for lectin activity and 56% for protein recovery. The efficiency of the process was confirmed by SDS-PAGE analysis.