Batch and continuous extraction of bromelain enzyme by reversed micelles

Detalhes bibliográficos
Autor(a) principal: Fileti,Ana Maria Frattini
Data de Publicação: 2009
Outros Autores: Fischer,Gilvan Anderson, Santana,José Carlos Curvelo, Tambourgi,Elias Basile
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021
Resumo: The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.
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spelling Batch and continuous extraction of bromelain enzyme by reversed micellesreversed micellesliquid-liquid extractionbromelainpineappleoptimizationsurface responseThe main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.Instituto de Tecnologia do Paraná - Tecpar2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021Brazilian Archives of Biology and Technology v.52 n.5 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000500021info:eu-repo/semantics/openAccessFileti,Ana Maria FrattiniFischer,Gilvan AndersonSantana,José Carlos CurveloTambourgi,Elias Basileeng2010-01-12T00:00:00Zoai:scielo:S1516-89132009000500021Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-01-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Batch and continuous extraction of bromelain enzyme by reversed micelles
title Batch and continuous extraction of bromelain enzyme by reversed micelles
spellingShingle Batch and continuous extraction of bromelain enzyme by reversed micelles
Fileti,Ana Maria Frattini
reversed micelles
liquid-liquid extraction
bromelain
pineapple
optimization
surface response
title_short Batch and continuous extraction of bromelain enzyme by reversed micelles
title_full Batch and continuous extraction of bromelain enzyme by reversed micelles
title_fullStr Batch and continuous extraction of bromelain enzyme by reversed micelles
title_full_unstemmed Batch and continuous extraction of bromelain enzyme by reversed micelles
title_sort Batch and continuous extraction of bromelain enzyme by reversed micelles
author Fileti,Ana Maria Frattini
author_facet Fileti,Ana Maria Frattini
Fischer,Gilvan Anderson
Santana,José Carlos Curvelo
Tambourgi,Elias Basile
author_role author
author2 Fischer,Gilvan Anderson
Santana,José Carlos Curvelo
Tambourgi,Elias Basile
author2_role author
author
author
dc.contributor.author.fl_str_mv Fileti,Ana Maria Frattini
Fischer,Gilvan Anderson
Santana,José Carlos Curvelo
Tambourgi,Elias Basile
dc.subject.por.fl_str_mv reversed micelles
liquid-liquid extraction
bromelain
pineapple
optimization
surface response
topic reversed micelles
liquid-liquid extraction
bromelain
pineapple
optimization
surface response
description The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132009000500021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.52 n.5 2009
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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