Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome

Detalhes bibliográficos
Autor(a) principal: Díaz-Abad, Lucía
Data de Publicação: 2022
Outros Autores: Bacco-Mannina, Natassia, Madeira, Fernando Miguel, EA, Serrao, Regalla, Aissa, Patrício, Ana R., Frade, Pedro R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.12/8870
Resumo: The fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts.
id RCAP_435df6250dc0ee50a84baad6e0714868
oai_identifier_str oai:repositorio.ispa.pt:10400.12/8870
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome16S rRNAMicrobiotaeDNAChelonia mydasMetabarcodingMacrophytesSea turtlesThe fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts.MDPI Multidisciplinary Digital Publishing InstituteRepositório do ISPADíaz-Abad, LucíaBacco-Mannina, NatassiaMadeira, Fernando MiguelEA, SerraoRegalla, AissaPatrício, Ana R.Frade, Pedro R.2022-12-20T16:40:35Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.12/8870eng10.3390/microorganisms101019882076260710.3390/microorganisms10101988info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-25T02:15:26Zoai:repositorio.ispa.pt:10400.12/8870Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:28:52.737137Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
title Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
spellingShingle Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
Díaz-Abad, Lucía
16S rRNA
Microbiota
eDNA
Chelonia mydas
Metabarcoding
Macrophytes
Sea turtles
title_short Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
title_full Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
title_fullStr Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
title_full_unstemmed Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
title_sort Red, gold and green: Microbial contribution of Rhodophyta and other algae to green turtle (Chelonia mydas) Gut Microbiome
author Díaz-Abad, Lucía
author_facet Díaz-Abad, Lucía
Bacco-Mannina, Natassia
Madeira, Fernando Miguel
EA, Serrao
Regalla, Aissa
Patrício, Ana R.
Frade, Pedro R.
author_role author
author2 Bacco-Mannina, Natassia
Madeira, Fernando Miguel
EA, Serrao
Regalla, Aissa
Patrício, Ana R.
Frade, Pedro R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório do ISPA
dc.contributor.author.fl_str_mv Díaz-Abad, Lucía
Bacco-Mannina, Natassia
Madeira, Fernando Miguel
EA, Serrao
Regalla, Aissa
Patrício, Ana R.
Frade, Pedro R.
dc.subject.por.fl_str_mv 16S rRNA
Microbiota
eDNA
Chelonia mydas
Metabarcoding
Macrophytes
Sea turtles
topic 16S rRNA
Microbiota
eDNA
Chelonia mydas
Metabarcoding
Macrophytes
Sea turtles
description The fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-20T16:40:35Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.12/8870
url http://hdl.handle.net/10400.12/8870
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3390/microorganisms10101988
20762607
10.3390/microorganisms10101988
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv MDPI Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799130746603962368