Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/18451 |
Resumo: | The fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts. |
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Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome16S rRNAMicrobiotaeDNAChelonia mydasMetabarcodingMacrophytesSea turtlesThe fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts.MDPISapientiaDíaz-Abad, LucíaBacco-Mannina, NatassiaMiguel Madeira, FernandoSerrao, EsterRegalla, AissaPatrício, Ana R.Rodrigues Frade, Pedro2022-10-28T08:54:16Z2022-10-082022-10-26T11:08:45Z2022-10-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/18451engMicroorganisms 10 (10): 1988 (2022)10.3390/microorganisms101019882076-2607info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-06T02:03:40Zoai:sapientia.ualg.pt:10400.1/18451Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:08:12.506809Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
title |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
spellingShingle |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome Díaz-Abad, Lucía 16S rRNA Microbiota eDNA Chelonia mydas Metabarcoding Macrophytes Sea turtles |
title_short |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
title_full |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
title_fullStr |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
title_full_unstemmed |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
title_sort |
Red, gold and green: microbial contribution of Rhodophyta and other Algae to Green Turtle (Chelonia mydas) Gut Microbiome |
author |
Díaz-Abad, Lucía |
author_facet |
Díaz-Abad, Lucía Bacco-Mannina, Natassia Miguel Madeira, Fernando Serrao, Ester Regalla, Aissa Patrício, Ana R. Rodrigues Frade, Pedro |
author_role |
author |
author2 |
Bacco-Mannina, Natassia Miguel Madeira, Fernando Serrao, Ester Regalla, Aissa Patrício, Ana R. Rodrigues Frade, Pedro |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Díaz-Abad, Lucía Bacco-Mannina, Natassia Miguel Madeira, Fernando Serrao, Ester Regalla, Aissa Patrício, Ana R. Rodrigues Frade, Pedro |
dc.subject.por.fl_str_mv |
16S rRNA Microbiota eDNA Chelonia mydas Metabarcoding Macrophytes Sea turtles |
topic |
16S rRNA Microbiota eDNA Chelonia mydas Metabarcoding Macrophytes Sea turtles |
description |
The fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-28T08:54:16Z 2022-10-08 2022-10-26T11:08:45Z 2022-10-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/18451 |
url |
http://hdl.handle.net/10400.1/18451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microorganisms 10 (10): 1988 (2022) 10.3390/microorganisms10101988 2076-2607 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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