Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4673 |
Resumo: | Recently, bone tissue engineering emerged as a viable therapeutic alternative, comprising bone implants and new personalized scaffolds to be used in bone replacement and regeneration. In this study, biocompatible scaffolds were produced by freeze-drying, using different formulations (chitosan, chitosan/gelatin, chitosan/β-TCP and chitosan/gelatin/β-TCP) to be used as temporary templates during bone tissue regeneration. Sample characterization was performed through attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray diffraction and energy dispersive spectroscopy analysis. Mechanical characterization and porosity analysis were performed through uniaxial compression test and liquid displacement method, respectively. In vitro studies were also done to evaluate the biomineralization activity and the cytotoxic profile of the scaffolds. Scanning electron and confocal microscopy analysis were used to study cell adhesion and proliferation at the scaffold surface and within their structure. Moreover, the antibacterial activity of the scaffolds was also evaluated through the agar diffusion method. Overall, the results obtained revealed that the produced scaffolds are bioactive and biocompatible, allow cell internalization and show antimicrobial activity against Staphylococcus aureus. Such, make these 3D structures as potential candidates for being used on the bone tissue regeneration, since they promote cell adhesion and proliferation and also prevent biofilm development at their surfaces, which is usually the main cause of implant failure. |
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Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regenerationAntibacterialScaffoldsFreeze-dryingBiomineralization bone regenerationBiocompatibilityRecently, bone tissue engineering emerged as a viable therapeutic alternative, comprising bone implants and new personalized scaffolds to be used in bone replacement and regeneration. In this study, biocompatible scaffolds were produced by freeze-drying, using different formulations (chitosan, chitosan/gelatin, chitosan/β-TCP and chitosan/gelatin/β-TCP) to be used as temporary templates during bone tissue regeneration. Sample characterization was performed through attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray diffraction and energy dispersive spectroscopy analysis. Mechanical characterization and porosity analysis were performed through uniaxial compression test and liquid displacement method, respectively. In vitro studies were also done to evaluate the biomineralization activity and the cytotoxic profile of the scaffolds. Scanning electron and confocal microscopy analysis were used to study cell adhesion and proliferation at the scaffold surface and within their structure. Moreover, the antibacterial activity of the scaffolds was also evaluated through the agar diffusion method. Overall, the results obtained revealed that the produced scaffolds are bioactive and biocompatible, allow cell internalization and show antimicrobial activity against Staphylococcus aureus. Such, make these 3D structures as potential candidates for being used on the bone tissue regeneration, since they promote cell adhesion and proliferation and also prevent biofilm development at their surfaces, which is usually the main cause of implant failure.ElsevieruBibliorumSerra, Inês Raquel TavaresFradique, Ricardo GilVallejo, Mariana C. da S.Correia, Tiago R.Miguel, Sónia P.Correia, Ilídio Joaquim Sobreira2018-03-21T09:20:26Z2015-05-302015-05-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4673engSerra, I.R., Fradique, R.G., Vallejo, M.C., Correia, T.R., Miguel, S.P. e Correia I.J. (2015) "Production and characterization of Chitosan/Gelatin/β-TCP scaffolds for improved bone tissue regeneration", Materials Science & Engineering C - Materials for Biological Applications, Vol. 55, pp.592-60410.1016/j.msec.2015.05.072metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T02:30:32Zoai:ubibliorum.ubi.pt:10400.6/4673Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:43.766575Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
title |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
spellingShingle |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration Serra, Inês Raquel Tavares Antibacterial Scaffolds Freeze-drying Biomineralization bone regeneration Biocompatibility |
title_short |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
title_full |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
title_fullStr |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
title_full_unstemmed |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
title_sort |
Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration |
author |
Serra, Inês Raquel Tavares |
author_facet |
Serra, Inês Raquel Tavares Fradique, Ricardo Gil Vallejo, Mariana C. da S. Correia, Tiago R. Miguel, Sónia P. Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Fradique, Ricardo Gil Vallejo, Mariana C. da S. Correia, Tiago R. Miguel, Sónia P. Correia, Ilídio Joaquim Sobreira |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Serra, Inês Raquel Tavares Fradique, Ricardo Gil Vallejo, Mariana C. da S. Correia, Tiago R. Miguel, Sónia P. Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
Antibacterial Scaffolds Freeze-drying Biomineralization bone regeneration Biocompatibility |
topic |
Antibacterial Scaffolds Freeze-drying Biomineralization bone regeneration Biocompatibility |
description |
Recently, bone tissue engineering emerged as a viable therapeutic alternative, comprising bone implants and new personalized scaffolds to be used in bone replacement and regeneration. In this study, biocompatible scaffolds were produced by freeze-drying, using different formulations (chitosan, chitosan/gelatin, chitosan/β-TCP and chitosan/gelatin/β-TCP) to be used as temporary templates during bone tissue regeneration. Sample characterization was performed through attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray diffraction and energy dispersive spectroscopy analysis. Mechanical characterization and porosity analysis were performed through uniaxial compression test and liquid displacement method, respectively. In vitro studies were also done to evaluate the biomineralization activity and the cytotoxic profile of the scaffolds. Scanning electron and confocal microscopy analysis were used to study cell adhesion and proliferation at the scaffold surface and within their structure. Moreover, the antibacterial activity of the scaffolds was also evaluated through the agar diffusion method. Overall, the results obtained revealed that the produced scaffolds are bioactive and biocompatible, allow cell internalization and show antimicrobial activity against Staphylococcus aureus. Such, make these 3D structures as potential candidates for being used on the bone tissue regeneration, since they promote cell adhesion and proliferation and also prevent biofilm development at their surfaces, which is usually the main cause of implant failure. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-30 2015-05-30T00:00:00Z 2018-03-21T09:20:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4673 |
url |
http://hdl.handle.net/10400.6/4673 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Serra, I.R., Fradique, R.G., Vallejo, M.C., Correia, T.R., Miguel, S.P. e Correia I.J. (2015) "Production and characterization of Chitosan/Gelatin/β-TCP scaffolds for improved bone tissue regeneration", Materials Science & Engineering C - Materials for Biological Applications, Vol. 55, pp.592-604 10.1016/j.msec.2015.05.072 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136354496413696 |