Use of Bacillus sp. in cleaning products

• Autor(a) principal: Trindade, Nuno de Almeida
• Data de Publicação: 2022
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/37887
• topic: Cleaning products; Bacillus; Microbial-based cleaning products; Sustainable cleaning

Cleaning products are important contributors to consumer exposure to chemicals, with the potential to cause respiratory and skin irritation and sensitization, negatively affecting health and the environment. In this context, microbial-based cleaning products emerge as a possible way to mitigate these damages, reducing the chemicals used in cleaning products without reducing their efficiency. The use of microorganisms as active components (designated as probiotics) is due to their ability to degrade a wide spectrum of substances. Several studies have evaluated the ability of microorganisms to degrade various organic compounds, which, combined with other characteristics such as the ability to compete with pathogenic microorganisms, have led to their incorporation into various cleaning products, with benefits both for cleaning efficiency and for the health. of consumers and environment. This work aimed to test the efficiency and stability of a Bacillus strain incorporated into the product Evogen GP 50x manufactured by the Croda Company and intended to be incorporated into cleaning products. For this, the ability of these bacteria to digest organic compounds (proteins, carbohydrates and lipids) often found on dirty surfaces was tested. The survival of these microorganisms in different cleaning matrices and storage conditions at the Mistolin Company was also evaluated, determining the shelf life of the formulated products and the cleaning efficiency after 10 weeks of storage. The strain tested showed medium to high capacity for digesting proteins, carbohydrates and lipids compared to other strains also belonging to the Bacilliaceae family. In the survival test, the bacteria remained alive and maintained their hydrolysis activity. Storage at room temperature and 4 ˚C proved to be suitable for the survival of microorganisms, but storage at 40 ˚C caused a high reduction in the of viable microorganisms. It was possible to observe that the inclusion of probiotics improved the efficiency of cleaning products in general.