Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/64408 |
Resumo: | In this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100\\%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma. |
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Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stromaBacterial celluloseCorneal stromaTissue engineeringBiocompatibilityScience & TechnologyIn this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100\\%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma.This work is supported by the National Natural Science Foundation of China (Grant Nos. 81200663, 51572187, 51973058, and 31870963), Tianjin Clinical Key Discipline Project (Grant No. TJLCZDXKM014), and Key Research and Development Program of Jiangxi Province (No. 20192ACB80008, 20171BBG70112).info:eu-repo/semantics/publishedVersionSpringerUniversidade do MinhoZhang, ChenCao, JingjieZhao, ShaozhenLuo, HonglinYang, ZhiweiGama, F. M.Zhang, QuanchaoSu, DanWan, Yizao2020-01-172020-01-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64408engZhang, Chen; Cao, Jingjie; Zhao, Shaozhen; Luo, Honglin; Yang, Zhiwei; Gama, F. M.; Zhang, Quanchao; Su, Dan; Wan, Yizao, Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma. Cellulose, 27, 2775-2784, 20200969-023910.1007/s10570-020-02979-0http://www.springer.com/chemistry/organic+chemistry/journal/10570info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:50:05Zoai:repositorium.sdum.uminho.pt:1822/64408Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:48:43.599178Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
title |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
spellingShingle |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma Zhang, Chen Bacterial cellulose Corneal stroma Tissue engineering Biocompatibility Science & Technology |
title_short |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
title_full |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
title_fullStr |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
title_full_unstemmed |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
title_sort |
Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma |
author |
Zhang, Chen |
author_facet |
Zhang, Chen Cao, Jingjie Zhao, Shaozhen Luo, Honglin Yang, Zhiwei Gama, F. M. Zhang, Quanchao Su, Dan Wan, Yizao |
author_role |
author |
author2 |
Cao, Jingjie Zhao, Shaozhen Luo, Honglin Yang, Zhiwei Gama, F. M. Zhang, Quanchao Su, Dan Wan, Yizao |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Zhang, Chen Cao, Jingjie Zhao, Shaozhen Luo, Honglin Yang, Zhiwei Gama, F. M. Zhang, Quanchao Su, Dan Wan, Yizao |
dc.subject.por.fl_str_mv |
Bacterial cellulose Corneal stroma Tissue engineering Biocompatibility Science & Technology |
topic |
Bacterial cellulose Corneal stroma Tissue engineering Biocompatibility Science & Technology |
description |
In this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100\\%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-17 2020-01-17T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64408 |
url |
http://hdl.handle.net/1822/64408 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zhang, Chen; Cao, Jingjie; Zhao, Shaozhen; Luo, Honglin; Yang, Zhiwei; Gama, F. M.; Zhang, Quanchao; Su, Dan; Wan, Yizao, Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma. Cellulose, 27, 2775-2784, 2020 0969-0239 10.1007/s10570-020-02979-0 http://www.springer.com/chemistry/organic+chemistry/journal/10570 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133066303635456 |