Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma

Detalhes bibliográficos
Autor(a) principal: Zhang, Chen
Data de Publicação: 2020
Outros Autores: Cao, Jingjie, Zhao, Shaozhen, Luo, Honglin, Yang, Zhiwei, Gama, F. M., Zhang, Quanchao, Su, Dan, Wan, Yizao
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/64408
Resumo: In this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100\\%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma.
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spelling Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stromaBacterial celluloseCorneal stromaTissue engineeringBiocompatibilityScience & TechnologyIn this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100\\%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma.This work is supported by the National Natural Science Foundation of China (Grant Nos. 81200663, 51572187, 51973058, and 31870963), Tianjin Clinical Key Discipline Project (Grant No. TJLCZDXKM014), and Key Research and Development Program of Jiangxi Province (No. 20192ACB80008, 20171BBG70112).info:eu-repo/semantics/publishedVersionSpringerUniversidade do MinhoZhang, ChenCao, JingjieZhao, ShaozhenLuo, HonglinYang, ZhiweiGama, F. M.Zhang, QuanchaoSu, DanWan, Yizao2020-01-172020-01-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64408engZhang, Chen; Cao, Jingjie; Zhao, Shaozhen; Luo, Honglin; Yang, Zhiwei; Gama, F. M.; Zhang, Quanchao; Su, Dan; Wan, Yizao, Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma. Cellulose, 27, 2775-2784, 20200969-023910.1007/s10570-020-02979-0http://www.springer.com/chemistry/organic+chemistry/journal/10570info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:50:05Zoai:repositorium.sdum.uminho.pt:1822/64408Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:48:43.599178Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
title Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
spellingShingle Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
Zhang, Chen
Bacterial cellulose
Corneal stroma
Tissue engineering
Biocompatibility
Science & Technology
title_short Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
title_full Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
title_fullStr Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
title_full_unstemmed Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
title_sort Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma
author Zhang, Chen
author_facet Zhang, Chen
Cao, Jingjie
Zhao, Shaozhen
Luo, Honglin
Yang, Zhiwei
Gama, F. M.
Zhang, Quanchao
Su, Dan
Wan, Yizao
author_role author
author2 Cao, Jingjie
Zhao, Shaozhen
Luo, Honglin
Yang, Zhiwei
Gama, F. M.
Zhang, Quanchao
Su, Dan
Wan, Yizao
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Zhang, Chen
Cao, Jingjie
Zhao, Shaozhen
Luo, Honglin
Yang, Zhiwei
Gama, F. M.
Zhang, Quanchao
Su, Dan
Wan, Yizao
dc.subject.por.fl_str_mv Bacterial cellulose
Corneal stroma
Tissue engineering
Biocompatibility
Science & Technology
topic Bacterial cellulose
Corneal stroma
Tissue engineering
Biocompatibility
Science & Technology
description In this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100\\%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-17
2020-01-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/64408
url http://hdl.handle.net/1822/64408
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Zhang, Chen; Cao, Jingjie; Zhao, Shaozhen; Luo, Honglin; Yang, Zhiwei; Gama, F. M.; Zhang, Quanchao; Su, Dan; Wan, Yizao, Biocompatibility evaluation of bacterial cellulose as a scaffold material for tissue-engineered corneal stroma. Cellulose, 27, 2775-2784, 2020
0969-0239
10.1007/s10570-020-02979-0
http://www.springer.com/chemistry/organic+chemistry/journal/10570
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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