Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/79146 |
Resumo: | The potential of nanomaterials in food technology is nowadays well-established. However, their commercial use requires a careful risk assessment, in particular concerning the fate of nanomaterials in the human body. Bacterial nanocellulose (BNC), a nanofibrillar polysaccharide, has been used as a food product for many years in Asia. However, given its nano-character, several toxicological studies must be performed, according to the European Food Safety Agency’s guidance. Those should especially answer the question of whether nanoparticulate cellulose is absorbed in the gastrointestinal tract. This raises the need to develop a screening technique capable of detecting isolated nanosized particles in biological tissues. Herein, the potential of a cellulose-binding module fused to a green fluorescent protein (GFP–CBM) to detect single bacterial cellulose nanocrystals (BCNC) obtained by acid hydrolysis was assessed. Adsorption studies were performed to characterize the interaction of GFP–CBM with BNC and BCNC. Correlative electron light microscopy was used to demonstrate that isolated BCNC may be detected by fluorescence microscopy. The uptake of BCNC by macrophages was also assessed. Finally, an exploratory 21-day repeated-dose study was performed, wherein Wistar rats were fed daily with BNC. The presence of BNC or BCNC throughout the GIT was observed only in the intestinal lumen, suggesting that cellulose particles were not absorbed. While a more comprehensive toxicological study is necessary, these results strengthen the idea that BNC can be considered a safe food additive. |
id |
RCAP_450117f5c469ce428bc75e2290129866 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/79146 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopybacterial nanocellulosebacterial cellulose nanocrystalscellulose binding modulefluorescence microscopygastrointestinal tractabsorptionfood additiveScience & TechnologyThe potential of nanomaterials in food technology is nowadays well-established. However, their commercial use requires a careful risk assessment, in particular concerning the fate of nanomaterials in the human body. Bacterial nanocellulose (BNC), a nanofibrillar polysaccharide, has been used as a food product for many years in Asia. However, given its nano-character, several toxicological studies must be performed, according to the European Food Safety Agency’s guidance. Those should especially answer the question of whether nanoparticulate cellulose is absorbed in the gastrointestinal tract. This raises the need to develop a screening technique capable of detecting isolated nanosized particles in biological tissues. Herein, the potential of a cellulose-binding module fused to a green fluorescent protein (GFP–CBM) to detect single bacterial cellulose nanocrystals (BCNC) obtained by acid hydrolysis was assessed. Adsorption studies were performed to characterize the interaction of GFP–CBM with BNC and BCNC. Correlative electron light microscopy was used to demonstrate that isolated BCNC may be detected by fluorescence microscopy. The uptake of BCNC by macrophages was also assessed. Finally, an exploratory 21-day repeated-dose study was performed, wherein Wistar rats were fed daily with BNC. The presence of BNC or BCNC throughout the GIT was observed only in the intestinal lumen, suggesting that cellulose particles were not absorbed. While a more comprehensive toxicological study is necessary, these results strengthen the idea that BNC can be considered a safe food additive.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI‐01‐0145‐FEDER‐006684) and BioTecNorte operation (NORTE‐01‐0145‐FEDER‐000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoMota, RenatoRodrigues, Ana CristinaSilva-Carvalho, RicardoCosta, LígiaMartins, DanielaSampaio, PaulaDourado, FernandoGama, F. M.2022-07-282022-07-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/79146engMota, Renato; Rodrigues, Ana Cristina; Silva-Carvalho, Ricardo; Costa, Lígia; Martins, Daniela; Sampaio, Paula; Dourado, Fernando; Gama, F. M., Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy. Nanomaterials, 12(15), 2605, 20222079-499110.3390/nano12152605https://www.mdpi.com/2079-4991/12/15/2605info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:10Zoai:repositorium.sdum.uminho.pt:1822/79146Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:34.721011Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
title |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
spellingShingle |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy Mota, Renato bacterial nanocellulose bacterial cellulose nanocrystals cellulose binding module fluorescence microscopy gastrointestinal tract absorption food additive Science & Technology |
title_short |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
title_full |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
title_fullStr |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
title_full_unstemmed |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
title_sort |
Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy |
author |
Mota, Renato |
author_facet |
Mota, Renato Rodrigues, Ana Cristina Silva-Carvalho, Ricardo Costa, Lígia Martins, Daniela Sampaio, Paula Dourado, Fernando Gama, F. M. |
author_role |
author |
author2 |
Rodrigues, Ana Cristina Silva-Carvalho, Ricardo Costa, Lígia Martins, Daniela Sampaio, Paula Dourado, Fernando Gama, F. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Mota, Renato Rodrigues, Ana Cristina Silva-Carvalho, Ricardo Costa, Lígia Martins, Daniela Sampaio, Paula Dourado, Fernando Gama, F. M. |
dc.subject.por.fl_str_mv |
bacterial nanocellulose bacterial cellulose nanocrystals cellulose binding module fluorescence microscopy gastrointestinal tract absorption food additive Science & Technology |
topic |
bacterial nanocellulose bacterial cellulose nanocrystals cellulose binding module fluorescence microscopy gastrointestinal tract absorption food additive Science & Technology |
description |
The potential of nanomaterials in food technology is nowadays well-established. However, their commercial use requires a careful risk assessment, in particular concerning the fate of nanomaterials in the human body. Bacterial nanocellulose (BNC), a nanofibrillar polysaccharide, has been used as a food product for many years in Asia. However, given its nano-character, several toxicological studies must be performed, according to the European Food Safety Agency’s guidance. Those should especially answer the question of whether nanoparticulate cellulose is absorbed in the gastrointestinal tract. This raises the need to develop a screening technique capable of detecting isolated nanosized particles in biological tissues. Herein, the potential of a cellulose-binding module fused to a green fluorescent protein (GFP–CBM) to detect single bacterial cellulose nanocrystals (BCNC) obtained by acid hydrolysis was assessed. Adsorption studies were performed to characterize the interaction of GFP–CBM with BNC and BCNC. Correlative electron light microscopy was used to demonstrate that isolated BCNC may be detected by fluorescence microscopy. The uptake of BCNC by macrophages was also assessed. Finally, an exploratory 21-day repeated-dose study was performed, wherein Wistar rats were fed daily with BNC. The presence of BNC or BCNC throughout the GIT was observed only in the intestinal lumen, suggesting that cellulose particles were not absorbed. While a more comprehensive toxicological study is necessary, these results strengthen the idea that BNC can be considered a safe food additive. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-28 2022-07-28T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/79146 |
url |
https://hdl.handle.net/1822/79146 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mota, Renato; Rodrigues, Ana Cristina; Silva-Carvalho, Ricardo; Costa, Lígia; Martins, Daniela; Sampaio, Paula; Dourado, Fernando; Gama, F. M., Tracking bacterial nanocellulose in animal tissues by fluorescence microscopy. Nanomaterials, 12(15), 2605, 2022 2079-4991 10.3390/nano12152605 https://www.mdpi.com/2079-4991/12/15/2605 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132952032968704 |