Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/6789 |
Resumo: | Random Amplified Polymorphic DNA (RAPD-PCR) technique was used in order to assess the level of genetic variability and genetic relationships among 24 Portuguese isolates of pinewood nematode, Bursaphelenchus xylophilus, representative of the fully affected area in Portugal. Two additional isolates of B. xylophilus, representing North America and East Asia were included, and B. mucronatus was used as out-group. Twenty-eight random primers generated a total of 640 DNA fragments. The Nei & Li similarity index revealed a high genetic similarity among the Portuguese isolates (above 90%). Hierarchical cluster analysis was performed to illustrate the relatedness among the isolates. No indication for separate groups among the Portuguese isolates was obtained, and the low level of genetic diversity strongly suggests that they were dispersed recently from a single introduction. The lack of apparent relationship between the genetic and the geographic matrices of the Portuguese isolates limits the use of this technique for following recent pathways of distribution. Genetic distance of the Portuguese isolates towards an isolate from China was much lower as compared to an isolate from the USA. This confirmed previous results suggesting an East Asian origin of the Portuguese B. xylophilus. |
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Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analysespinewood NematodeDNA fingerprintingRAPDsimilitaryRandom Amplified Polymorphic DNA (RAPD-PCR) technique was used in order to assess the level of genetic variability and genetic relationships among 24 Portuguese isolates of pinewood nematode, Bursaphelenchus xylophilus, representative of the fully affected area in Portugal. Two additional isolates of B. xylophilus, representing North America and East Asia were included, and B. mucronatus was used as out-group. Twenty-eight random primers generated a total of 640 DNA fragments. The Nei & Li similarity index revealed a high genetic similarity among the Portuguese isolates (above 90%). Hierarchical cluster analysis was performed to illustrate the relatedness among the isolates. No indication for separate groups among the Portuguese isolates was obtained, and the low level of genetic diversity strongly suggests that they were dispersed recently from a single introduction. The lack of apparent relationship between the genetic and the geographic matrices of the Portuguese isolates limits the use of this technique for following recent pathways of distribution. Genetic distance of the Portuguese isolates towards an isolate from China was much lower as compared to an isolate from the USA. This confirmed previous results suggesting an East Asian origin of the Portuguese B. xylophilus.2012-12-10T16:14:31Z2012-12-102007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/6789http://hdl.handle.net/10174/6789eng118-12639ICAAMpvieira@uevora.ptndmmota@uevora.pt218Vieira, PauloBurgermeister, WolfgangMota, Manuelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:46:20Zoai:dspace.uevora.pt:10174/6789Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:01:25.701411Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
title |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
spellingShingle |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses Vieira, Paulo pinewood Nematode DNA fingerprinting RAPD similitary |
title_short |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
title_full |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
title_fullStr |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
title_full_unstemmed |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
title_sort |
Lack of genetic variation of Bursaphelencus xylophylus in Portugal revealed by RAPD-PCR analyses |
author |
Vieira, Paulo |
author_facet |
Vieira, Paulo Burgermeister, Wolfgang Mota, Manuel |
author_role |
author |
author2 |
Burgermeister, Wolfgang Mota, Manuel |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Vieira, Paulo Burgermeister, Wolfgang Mota, Manuel |
dc.subject.por.fl_str_mv |
pinewood Nematode DNA fingerprinting RAPD similitary |
topic |
pinewood Nematode DNA fingerprinting RAPD similitary |
description |
Random Amplified Polymorphic DNA (RAPD-PCR) technique was used in order to assess the level of genetic variability and genetic relationships among 24 Portuguese isolates of pinewood nematode, Bursaphelenchus xylophilus, representative of the fully affected area in Portugal. Two additional isolates of B. xylophilus, representing North America and East Asia were included, and B. mucronatus was used as out-group. Twenty-eight random primers generated a total of 640 DNA fragments. The Nei & Li similarity index revealed a high genetic similarity among the Portuguese isolates (above 90%). Hierarchical cluster analysis was performed to illustrate the relatedness among the isolates. No indication for separate groups among the Portuguese isolates was obtained, and the low level of genetic diversity strongly suggests that they were dispersed recently from a single introduction. The lack of apparent relationship between the genetic and the geographic matrices of the Portuguese isolates limits the use of this technique for following recent pathways of distribution. Genetic distance of the Portuguese isolates towards an isolate from China was much lower as compared to an isolate from the USA. This confirmed previous results suggesting an East Asian origin of the Portuguese B. xylophilus. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01T00:00:00Z 2012-12-10T16:14:31Z 2012-12-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/6789 http://hdl.handle.net/10174/6789 |
url |
http://hdl.handle.net/10174/6789 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
118-126 39 ICAAM pvieira@uevora.pt nd mmota@uevora.pt 218 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136497409982464 |