Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/139239 |
Resumo: | Funding Information: We acknowledge to Unidade Investigação Patobiologia Molecular (UIPM) facilities and to Pathology Department of Instituto Português de Oncologia Francisco Gentil de Lisboa (IPOLFG). We acknowledge funding from the iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES), through national funds”. Funding Information: The authors declare that the article was partially funded by iNOVA4Health—UIDB/04462/2020 and iNOVA4Health—UIDP/04462/2020. |
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Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO433D modelsCell linesChemoresistanceOvarian cancerGeneticsOncologyCancer ResearchSDG 3 - Good Health and Well-beingFunding Information: We acknowledge to Unidade Investigação Patobiologia Molecular (UIPM) facilities and to Pathology Department of Instituto Português de Oncologia Francisco Gentil de Lisboa (IPOLFG). We acknowledge funding from the iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES), through national funds”. Funding Information: The authors declare that the article was partially funded by iNOVA4Health—UIDB/04462/2020 and iNOVA4Health—UIDP/04462/2020.BACKGROUND: Epithelial ovarian cancer (EOC) is an aggressive and lethal malignancy and novel EOC cell lines with detailed characterization are needed, to provide researchers with diverse helpful resources to study EOC biological processes and cancer experimental therapies. METHODS: The IPO43 cell line was established from the ascitic fluid of a patient with a diagnosis of high-grade serous carcinoma (HGSC) of the ovary, previously treated with chemotherapy. Cell immortalization was achieved in 2D cell culture and growth obtained in 2D and 3D cell cultures. The characterization of immortalized cells was done by immunocytochemistry, flow cytometry, cell proliferation, chromosomal Comparative Genomic Hybridization (cCGH), STR profile and Next Generation Sequencing (NGS). RESULTS: Characterization studies confirmed that IPO43 cell line is of EOC origin and maintains morphological and molecular features of the primary tumor. cCGH analysis showed a complex profile with gains and losses of specific DNA regions in both primary ascitic fluid and cell line IPO43. The cell line was successfully grown in a 3D system which allows its future application in more complex assays than those performed in 2D models. IPO43 cell line is resistant to standard drug treatment in vitro. CONCLUSIONS: IPO43 is available for public research and we hope it can contribute to enrich the in vitro models addressing EOC heterogeneity, being useful to investigate EOC and to develop new therapeutic modalities.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNSilva, FernandaCoelho, FilipaPeixoto, AnaPinto, PedroMartins, CarmoFrombach, Ann SophieSanto, Vítor E.Brito, CatarinaGuimarães, AntónioFélix, Ana2022-06-01T22:27:01Z2022-04-302022-04-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/139239eng1475-2867PURE: 43788069https://doi.org/10.1186/s12935-022-02600-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:16:34Zoai:run.unl.pt:10362/139239Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:49:22.355900Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| title |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| spellingShingle |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 Silva, Fernanda 3D models Cell lines Chemoresistance Ovarian cancer Genetics Oncology Cancer Research SDG 3 - Good Health and Well-being |
| title_short |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| title_full |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| title_fullStr |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| title_full_unstemmed |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| title_sort |
Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43 |
| author |
Silva, Fernanda |
| author_facet |
Silva, Fernanda Coelho, Filipa Peixoto, Ana Pinto, Pedro Martins, Carmo Frombach, Ann Sophie Santo, Vítor E. Brito, Catarina Guimarães, António Félix, Ana |
| author_role |
author |
| author2 |
Coelho, Filipa Peixoto, Ana Pinto, Pedro Martins, Carmo Frombach, Ann Sophie Santo, Vítor E. Brito, Catarina Guimarães, António Félix, Ana |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
| dc.contributor.author.fl_str_mv |
Silva, Fernanda Coelho, Filipa Peixoto, Ana Pinto, Pedro Martins, Carmo Frombach, Ann Sophie Santo, Vítor E. Brito, Catarina Guimarães, António Félix, Ana |
| dc.subject.por.fl_str_mv |
3D models Cell lines Chemoresistance Ovarian cancer Genetics Oncology Cancer Research SDG 3 - Good Health and Well-being |
| topic |
3D models Cell lines Chemoresistance Ovarian cancer Genetics Oncology Cancer Research SDG 3 - Good Health and Well-being |
| description |
Funding Information: We acknowledge to Unidade Investigação Patobiologia Molecular (UIPM) facilities and to Pathology Department of Instituto Português de Oncologia Francisco Gentil de Lisboa (IPOLFG). We acknowledge funding from the iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES), through national funds”. Funding Information: The authors declare that the article was partially funded by iNOVA4Health—UIDB/04462/2020 and iNOVA4Health—UIDP/04462/2020. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-06-01T22:27:01Z 2022-04-30 2022-04-30T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/139239 |
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http://hdl.handle.net/10362/139239 |
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eng |
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eng |
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1475-2867 PURE: 43788069 https://doi.org/10.1186/s12935-022-02600-3 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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