Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex

Detalhes bibliográficos
Autor(a) principal: de Beer, J.L.
Data de Publicação: 2014
Outros Autores: Ködmön, C., van Ingen, J., Supply, P., van Soolingen, D., Global Network for the Molecular Surveillance of Tuberculosis 2010
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/4043
Resumo: Global Network for the Molecular Surveillance of Tuberculosis 2010: A. Miranda (Tuberculosis Laboratory of the National Institute of Health, Porto, Portugal)
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spelling Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complexVNTRMLVATuberculosisReproducibilityProficiencyQuality ControlTandem RepeatsInfecções RespiratóriasGlobal Network for the Molecular Surveillance of Tuberculosis 2010: A. Miranda (Tuberculosis Laboratory of the National Institute of Health, Porto, Portugal)BACKGROUND: The quality of variable number of tandem repeats (VNTR) typing of Mycobacterium tuberculosis was first investigated in 2009 in 37 laboratories worldwide. The results revealed an inter- and intra-laboratory reproducibility of respectively 60% and 72%. These data spurred an improvement in laboratory-specific assays and global standardisation of VNTR typing. OBJECTIVE: To measure the effects of the technical improvements and increased standardisation, a test panel consisting of 30 M. tuberculosis complex DNA samples was distributed for VNTR typing in 41 participating laboratories from 36 countries. RESULTS: The inter- and intra-laboratory reproducibil- ity increased overall to respectively 78% and 88%. The 33 laboratories that participated in both the first and second proficiency studies improved their inter- and intra-laboratory reproducibility from 62% and 72% to respectively 79% and 88%. The largest improvement in reproducibility was detected in 10 laboratories that use an in-house polymerase chain reaction technique and perform amplicon sizing using gel electrophoresis. Detailed error analysis revealed a reduction in the number of systematic errors, sample exchange events and non-amplifiable loci. CONCLUSION: This second worldwide proficiency study indicates a substantial increase in the reproduc- ibility of VNTR typing of M. tuberculosis. This will contribute to a more meaningful interpretation of molecular epidemiological and phylogenetic studies on the M. tuberculosis complex.International Union Against Tuberculosis and Lung DiseaseRepositório Científico do Instituto Nacional de Saúdede Beer, J.L.Ködmön, C.van Ingen, J.Supply, P.van Soolingen, D.Global Network for the Molecular Surveillance of Tuberculosis 20102016-10-06T15:35:59Z2014-052014-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4043engInt J Tuberc Lung Dis. 2014 May;18(5):594-600. doi: 10.5588/ijtld.13.05311027-371910.5588/ijtld.13.0531info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:51Zoai:repositorio.insa.pt:10400.18/4043Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:24.251687Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
title Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
spellingShingle Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
de Beer, J.L.
VNTR
MLVA
Tuberculosis
Reproducibility
Proficiency
Quality Control
Tandem Repeats
Infecções Respiratórias
title_short Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
title_full Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
title_fullStr Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
title_full_unstemmed Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
title_sort Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
author de Beer, J.L.
author_facet de Beer, J.L.
Ködmön, C.
van Ingen, J.
Supply, P.
van Soolingen, D.
Global Network for the Molecular Surveillance of Tuberculosis 2010
author_role author
author2 Ködmön, C.
van Ingen, J.
Supply, P.
van Soolingen, D.
Global Network for the Molecular Surveillance of Tuberculosis 2010
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv de Beer, J.L.
Ködmön, C.
van Ingen, J.
Supply, P.
van Soolingen, D.
Global Network for the Molecular Surveillance of Tuberculosis 2010
dc.subject.por.fl_str_mv VNTR
MLVA
Tuberculosis
Reproducibility
Proficiency
Quality Control
Tandem Repeats
Infecções Respiratórias
topic VNTR
MLVA
Tuberculosis
Reproducibility
Proficiency
Quality Control
Tandem Repeats
Infecções Respiratórias
description Global Network for the Molecular Surveillance of Tuberculosis 2010: A. Miranda (Tuberculosis Laboratory of the National Institute of Health, Porto, Portugal)
publishDate 2014
dc.date.none.fl_str_mv 2014-05
2014-05-01T00:00:00Z
2016-10-06T15:35:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/4043
url http://hdl.handle.net/10400.18/4043
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Tuberc Lung Dis. 2014 May;18(5):594-600. doi: 10.5588/ijtld.13.0531
1027-3719
10.5588/ijtld.13.0531
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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