First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains

Detalhes bibliográficos
Autor(a) principal: de Beer, Jessica L.
Data de Publicação: 2012
Outros Autores: Kremer, Kristin, Ködmön, Csaba, Supply, Philip, van Soolingen, Dick
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19009
Resumo: National Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands.
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spelling de Beer, Jessica L.Kremer, KristinKödmön, CsabaSupply, Philipvan Soolingen, Dick2017-05-25T16:29:55Z2017-05-25T16:29:55Z2012de Beer, Jessica L. et al. First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains. Journal of Clinical Microbiology, v.50, n.3, p.662-669, Mar. 2012.0095-1137https://www.arca.fiocruz.br/handle/icict/1900910.1128/JCM.00607-111098-660XengAmerican Society for MicrobiologyMycobacterium tuberculosisEstudo mundial de ProficiênciaVariáveis tipagensMycobacterium tuberculosisProficiency StudyVariable-Number Tandem-Repeat TypingFirst worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands.World Health Organization. Regional Office for Europe. Health Security & Environment. Division of Communicable Diseases. Tuberculosis and M/XDR-TB Programme. Copenhagen, Denmark..European Centre for Disease Prevention and Control. Tuberculosis Programme. Surveillance Unit. Stockholm, Sweden.Université Lille Nord de France and Institut Pasteur de Lille. Center for Infection and Immunity of Lille, INSERM U1019-CNRS UMR 8204.. Lille, France / GenoScreen. Lille, France.National Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands / Radboud University Nijmegen Medical Centre, Dekkerswald. Medical Microbiology. Nijmegen, Netherlands.Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19009/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALphilip_suffys_etal_IOC_2012.pdfphilip_suffys_etal_IOC_2012.pdfapplication/pdf320574https://www.arca.fiocruz.br/bitstream/icict/19009/2/philip_suffys_etal_IOC_2012.pdf4a7dd2dda5fd74c2c53454f832284a49MD52TEXTphilip_suffys_etal_IOC_2012.pdf.txtphilip_suffys_etal_IOC_2012.pdf.txtExtracted texttext/plain48606https://www.arca.fiocruz.br/bitstream/icict/19009/3/philip_suffys_etal_IOC_2012.pdf.txt294fec9ac76de6b1dceae7628dda24d8MD53icict/190092018-08-15 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dc.title.pt_BR.fl_str_mv First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
title First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
spellingShingle First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
de Beer, Jessica L.
Mycobacterium tuberculosis
Estudo mundial de Proficiência
Variáveis tipagens
Mycobacterium tuberculosis
Proficiency Study
Variable-Number Tandem-Repeat Typing
title_short First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
title_full First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
title_fullStr First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
title_full_unstemmed First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
title_sort First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
author de Beer, Jessica L.
author_facet de Beer, Jessica L.
Kremer, Kristin
Ködmön, Csaba
Supply, Philip
van Soolingen, Dick
author_role author
author2 Kremer, Kristin
Ködmön, Csaba
Supply, Philip
van Soolingen, Dick
author2_role author
author
author
author
dc.contributor.author.fl_str_mv de Beer, Jessica L.
Kremer, Kristin
Ködmön, Csaba
Supply, Philip
van Soolingen, Dick
dc.subject.other.pt_BR.fl_str_mv Mycobacterium tuberculosis
Estudo mundial de Proficiência
Variáveis tipagens
topic Mycobacterium tuberculosis
Estudo mundial de Proficiência
Variáveis tipagens
Mycobacterium tuberculosis
Proficiency Study
Variable-Number Tandem-Repeat Typing
dc.subject.en.pt_BR.fl_str_mv Mycobacterium tuberculosis
Proficiency Study
Variable-Number Tandem-Repeat Typing
description National Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-05-25T16:29:55Z
dc.date.available.fl_str_mv 2017-05-25T16:29:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv de Beer, Jessica L. et al. First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains. Journal of Clinical Microbiology, v.50, n.3, p.662-669, Mar. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19009
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.00607-11
dc.identifier.eissn.none.fl_str_mv 1098-660X
identifier_str_mv de Beer, Jessica L. et al. First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains. Journal of Clinical Microbiology, v.50, n.3, p.662-669, Mar. 2012.
0095-1137
10.1128/JCM.00607-11
1098-660X
url https://www.arca.fiocruz.br/handle/icict/19009
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/19009/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/19009/3/philip_suffys_etal_IOC_2012.pdf.txt
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