Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/23061 |
Resumo: | Olive anthracnose, caused by Colletotrichum spp., is one of the most damaging fruit diseases in olive crops worldwide. Their control is very difficult and relies mostly on the use of copper-based fungicides. The plant-associate fungal community has been increasingly recognized for playing an important role in plant health. Thus, in this work, the leaf-associated fungi of olive cultivars with different levels of resistance to anthracnose were characterized to identify potential fungi implicated in host resistance. A culture-dependent approach was used to assess both epiphytic and endophytic fungal communities of leaves of olive cultivars Madural (susceptible to anthracnose) and Cobrançosa (moderately tolerant), and the isolates obtained were identified by rRNA sequencing. Overall, Ascomycota phylum and Aureobasidium genus were the most dominant, being epiphytes significantly more diverse and abundant than endophytes. Among the genera identified in the most-resistant cultivar, Aspergillus and Pseudocercospora were the most frequently isolated within epiphytic and endophytic communities, respectively; whereas in the susceptible cultivar, Aureobasidium and Didymocyrtis, were the most frequently isolated within epiphytic and endophytic communities, respectively. The host plant (at cultivar level) had a structuring effect on the composition of fungal communities of leaves, being this effect greater on epiphytes than on endophytes. Thus, it is likely that each cultivar selects specific fungal taxa, which may lead to specific feedbacks on fitness of plant genotypes. In total, 20 fungal taxa (15 endophytes and 5 epiphytes) were responsible for more than 50% of the dissimilarity found on fungal community between cultivars. Among these, three taxa from the Phaeomoniellaceae family and one unidentified fungus, were the most discriminant. Their functional role needs to be studied in the future, because they might be important in conferring host plant resistance towards anthracnose. |
id |
RCAP_46c7d3d1eefd8fc9c2ae3659c261fccb |
---|---|
oai_identifier_str |
oai:bibliotecadigital.ipb.pt:10198/23061 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnoseOlea europaeaColletotrichumEndophytesEpiphytesHost specificityDomínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e AlimentarOlive anthracnose, caused by Colletotrichum spp., is one of the most damaging fruit diseases in olive crops worldwide. Their control is very difficult and relies mostly on the use of copper-based fungicides. The plant-associate fungal community has been increasingly recognized for playing an important role in plant health. Thus, in this work, the leaf-associated fungi of olive cultivars with different levels of resistance to anthracnose were characterized to identify potential fungi implicated in host resistance. A culture-dependent approach was used to assess both epiphytic and endophytic fungal communities of leaves of olive cultivars Madural (susceptible to anthracnose) and Cobrançosa (moderately tolerant), and the isolates obtained were identified by rRNA sequencing. Overall, Ascomycota phylum and Aureobasidium genus were the most dominant, being epiphytes significantly more diverse and abundant than endophytes. Among the genera identified in the most-resistant cultivar, Aspergillus and Pseudocercospora were the most frequently isolated within epiphytic and endophytic communities, respectively; whereas in the susceptible cultivar, Aureobasidium and Didymocyrtis, were the most frequently isolated within epiphytic and endophytic communities, respectively. The host plant (at cultivar level) had a structuring effect on the composition of fungal communities of leaves, being this effect greater on epiphytes than on endophytes. Thus, it is likely that each cultivar selects specific fungal taxa, which may lead to specific feedbacks on fitness of plant genotypes. In total, 20 fungal taxa (15 endophytes and 5 epiphytes) were responsible for more than 50% of the dissimilarity found on fungal community between cultivars. Among these, three taxa from the Phaeomoniellaceae family and one unidentified fungus, were the most discriminant. Their functional role needs to be studied in the future, because they might be important in conferring host plant resistance towards anthracnose.A gafa, causada por diversos fungos do género Colletotrichum, é das doenças que mais prejuízos causa nos olivais em todo o mundo. A sua luta é muito difícil e é feita com recurso a fungicidas à base de cobre. A comunidade fúngica associada às plantas tem sido cada vez mais reconhecida por desempenhar um papel importante na sanidade das plantas. Assim, neste trabalho, foram caracterizados fungos associados a folhas de cultivares de oliveira, com diferentes níveis de suscetibilidade à gafa, para identificar fungos potencialmente responsáveis por estas diferenças de resistência. Para tal, recorreu-se ao método dependente de cultivo para avaliar as comunidades fúngicas epífitas e endófitas em folhas das cultivares Madural (suscetível à gafa) e Cobrançosa (moderadamente tolerante), tendo os isolados obtidos sido identificados pela sequenciação da região ITS do rRNA nuclear. Globalmente, o filo Ascomycota e o género Aureobasidium foram os mais dominantes, enquanto que os epífitos revelaram ser significativamente mais diversos e abundantes do que os endófitos. Entre os géneros identificados na cultivar mais resistente, Aspergillus e Pseudocercospora foram os mais abundantes dentro das comunidades fúngicas epífitas e endofíticas, respetivamente; já na cultivar suscetível, Aureobasidium e Didymocyrtis, foram os géneros mais frequentemente isolados dentro das comunidades fúngicas epífitas e endofíticas, respetivamente. O genótipo da planta (ao nível da cultivar) teve um efeito significativo na composição da comunidade fúngica das folhas, sendo este efeito superior nos epífitos face aos endófitos. Parece, assim, que cada cultivar possui a capacidade de selecione uma espécie específica, que poderá ter repercussões positivas na planta hospedeira. No total, 20 espécies fúngicas (15 endófitos e 5 epífitos) foram responsáveis por mais de 50% da dissimilaridade encontrada na comunidade fúngica entre as cultivares. De entre estas, três espécies da família Phaeomoniellaceae e um fungo não identificado, foram os mais discriminantes. A função destas espécies na promoção de resistência na oliveira deverá ser estudada no futuro.This work is supported by FEDER funds through the COMPETE (Operational Program for Competitiveness Factors) and by National funds through the FCT (Foundation for Science and Technology) in the scope of the project POCI-01-0145-FEDER-031133 “MicOlives - Exploiting plant induced resistance by beneficial fungi as a new sustainable approach to olive crop protection”.Baptista, PaulaRamos, Vítor Manuel CapelaBiblioteca Digital do IPBBahri, Hamdi2021-01-07T11:48:05Z202020192020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10198/23061TID:202573834enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:51:36Zoai:bibliotecadigital.ipb.pt:10198/23061Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:14:07.834565Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
title |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
spellingShingle |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose Bahri, Hamdi Olea europaea Colletotrichum Endophytes Epiphytes Host specificity Domínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e Alimentar |
title_short |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
title_full |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
title_fullStr |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
title_full_unstemmed |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
title_sort |
Characterization of leaf-associated fungi from olive cultivars with different levels of resistance to anthracnose |
author |
Bahri, Hamdi |
author_facet |
Bahri, Hamdi |
author_role |
author |
dc.contributor.none.fl_str_mv |
Baptista, Paula Ramos, Vítor Manuel Capela Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Bahri, Hamdi |
dc.subject.por.fl_str_mv |
Olea europaea Colletotrichum Endophytes Epiphytes Host specificity Domínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e Alimentar |
topic |
Olea europaea Colletotrichum Endophytes Epiphytes Host specificity Domínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e Alimentar |
description |
Olive anthracnose, caused by Colletotrichum spp., is one of the most damaging fruit diseases in olive crops worldwide. Their control is very difficult and relies mostly on the use of copper-based fungicides. The plant-associate fungal community has been increasingly recognized for playing an important role in plant health. Thus, in this work, the leaf-associated fungi of olive cultivars with different levels of resistance to anthracnose were characterized to identify potential fungi implicated in host resistance. A culture-dependent approach was used to assess both epiphytic and endophytic fungal communities of leaves of olive cultivars Madural (susceptible to anthracnose) and Cobrançosa (moderately tolerant), and the isolates obtained were identified by rRNA sequencing. Overall, Ascomycota phylum and Aureobasidium genus were the most dominant, being epiphytes significantly more diverse and abundant than endophytes. Among the genera identified in the most-resistant cultivar, Aspergillus and Pseudocercospora were the most frequently isolated within epiphytic and endophytic communities, respectively; whereas in the susceptible cultivar, Aureobasidium and Didymocyrtis, were the most frequently isolated within epiphytic and endophytic communities, respectively. The host plant (at cultivar level) had a structuring effect on the composition of fungal communities of leaves, being this effect greater on epiphytes than on endophytes. Thus, it is likely that each cultivar selects specific fungal taxa, which may lead to specific feedbacks on fitness of plant genotypes. In total, 20 fungal taxa (15 endophytes and 5 epiphytes) were responsible for more than 50% of the dissimilarity found on fungal community between cultivars. Among these, three taxa from the Phaeomoniellaceae family and one unidentified fungus, were the most discriminant. Their functional role needs to be studied in the future, because they might be important in conferring host plant resistance towards anthracnose. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2020 2020-01-01T00:00:00Z 2021-01-07T11:48:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/23061 TID:202573834 |
url |
http://hdl.handle.net/10198/23061 |
identifier_str_mv |
TID:202573834 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799135420784574464 |