Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8574 |
Resumo: | This article belongs to the Special Issue Discovery of Bioactive Ingredients from Natural Products III. |
| id |
RCAP_475db098970dd0369fed7dcfb8c88ce3 |
|---|---|
| oai_identifier_str |
oai:repositorio.insa.pt:10400.18/8574 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf ExtractsAloe-emodinAntimicrobialAntioxidantAsphodelusChemical ProfileHerbal MedicinesPreclinical SafetyStaphylococcus EpidermidisResistência aos AntimicrobianosThis article belongs to the Special Issue Discovery of Bioactive Ingredients from Natural Products III.The leaves of Asphodelus bento-rainhae subsp. bento-rainhae, an endemic Portuguese species, and Asphodelus macrocarpus subsp. macrocarpus have been used as food, and traditionally as medicine, for treating ulcers, urinary tract, and inflammatory disorders. The present study aims to establish the phytochemical profile of the main secondary metabolites, together with the antimicrobial, antioxidant and toxicity assessments of both Asphodelus leaf 70% ethanol extracts. Phytochemical screenings were conducted by the TLC and LC-UV/DAD-ESI/MS chromatographic technique, and quantification of the leading chemical classes was performed by spectrophotometric methods. Liquid-liquid partitions of crude extracts were obtained using ethyl ether, ethyl acetate, and water. For in vitro evaluations of antimicrobial activity, the broth microdilution method, and for the antioxidant activity, the FRAP and DPPH methods were used. Genotoxicity and cytotoxicity were assessed by Ames and MTT tests, respectively. Twelve known compounds including neochlorogenic acid, chlorogenic acid, caffeic acid, isoorientin, p-coumaric acid, isovitexin, ferulic acid, luteolin, aloe-emodin, diosmetin, chrysophanol, and β-sitosterol were identified as the main marker compounds, and terpenoids and condensed tannins were found to be the major class of secondary metabolites of both medicinal plants. The ethyl ether fractions demonstrated the highest antibacterial activity against all the Gram-positive microorganisms, (MIC value of 62 to 1000 µg/mL), with aloe-emodin as one of the main marker compounds highly active against Staphylococcus epidermidis (MIC value of 0.8 to 1.6 µg/mL). Ethyl acetate fractions exhibited the highest antioxidant activity (IC50 of 800 to 1200 µg/mL, respectively). No cytotoxicity (up to 1000 µg/mL) or genotoxicity/mutagenicity (up to 5 mg/plate, with/without metabolic activation) were detected. The obtained results contribute to the knowledge of the value and safety of the studied species as herbal medicines.This research was funded by the Foundation for Science and Technology (FCT, Portugal) through national funds FCT/MCTES to iMed.ULisboa (UIDP/04138/2020, UIDB/04138/2020) and MEtRICs (UIDP/04077/2020, UIDB/04077/2020) research projects, as well as doctoral scholarship (SFRH/BD/125310/2016) granted to the first author.MDPIRepositório Científico do Instituto Nacional de SaúdeMalmir, MaryamLima, KatelenePóvoas Camões, SérgioManageiro, VeraDuarte, Maria PaulaPaiva Miranda, JoanaSerrano, RitaMoreira da Silva, IsabelSilva Lima, BeatrizCaniça, ManuelaSilva, Olga2023-03-20T14:31:25Z2023-03-042023-03-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8574engMolecules. 2023 Mar 4;28(5):2372. doi: 10.3390/molecules28052372.1420-304910.3390/molecules28052372info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:38Zoai:repositorio.insa.pt:10400.18/8574Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:13.069910Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| spellingShingle |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts Malmir, Maryam Aloe-emodin Antimicrobial Antioxidant Asphodelus Chemical Profile Herbal Medicines Preclinical Safety Staphylococcus Epidermidis Resistência aos Antimicrobianos |
| title_short |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_full |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_fullStr |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_full_unstemmed |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_sort |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| author |
Malmir, Maryam |
| author_facet |
Malmir, Maryam Lima, Katelene Póvoas Camões, Sérgio Manageiro, Vera Duarte, Maria Paula Paiva Miranda, Joana Serrano, Rita Moreira da Silva, Isabel Silva Lima, Beatriz Caniça, Manuela Silva, Olga |
| author_role |
author |
| author2 |
Lima, Katelene Póvoas Camões, Sérgio Manageiro, Vera Duarte, Maria Paula Paiva Miranda, Joana Serrano, Rita Moreira da Silva, Isabel Silva Lima, Beatriz Caniça, Manuela Silva, Olga |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Malmir, Maryam Lima, Katelene Póvoas Camões, Sérgio Manageiro, Vera Duarte, Maria Paula Paiva Miranda, Joana Serrano, Rita Moreira da Silva, Isabel Silva Lima, Beatriz Caniça, Manuela Silva, Olga |
| dc.subject.por.fl_str_mv |
Aloe-emodin Antimicrobial Antioxidant Asphodelus Chemical Profile Herbal Medicines Preclinical Safety Staphylococcus Epidermidis Resistência aos Antimicrobianos |
| topic |
Aloe-emodin Antimicrobial Antioxidant Asphodelus Chemical Profile Herbal Medicines Preclinical Safety Staphylococcus Epidermidis Resistência aos Antimicrobianos |
| description |
This article belongs to the Special Issue Discovery of Bioactive Ingredients from Natural Products III. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-03-20T14:31:25Z 2023-03-04 2023-03-04T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/8574 |
| url |
http://hdl.handle.net/10400.18/8574 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Molecules. 2023 Mar 4;28(5):2372. doi: 10.3390/molecules28052372. 1420-3049 10.3390/molecules28052372 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799132177622892544 |