Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/158393 |
Resumo: | Publisher Copyright: © 2023 by the authors. |
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Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf ExtractsAloe-emodinantimicrobialantioxidantAsphodeluschemical profileherbal medicinespreclinical safetyStaphylococcus epidermidisAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistryPublisher Copyright: © 2023 by the authors.The leaves of Asphodelus bento-rainhae subsp. bento-rainhae, an endemic Portuguese species, and Asphodelus macrocarpus subsp. macrocarpus have been used as food, and traditionally as medicine, for treating ulcers, urinary tract, and inflammatory disorders. The present study aims to establish the phytochemical profile of the main secondary metabolites, together with the antimicrobial, antioxidant and toxicity assessments of both Asphodelus leaf 70% ethanol extracts. Phytochemical screenings were conducted by the TLC and LC-UV/DAD-ESI/MS chromatographic technique, and quantification of the leading chemical classes was performed by spectrophotometric methods. Liquid-liquid partitions of crude extracts were obtained using ethyl ether, ethyl acetate, and water. For in vitro evaluations of antimicrobial activity, the broth microdilution method, and for the antioxidant activity, the FRAP and DPPH methods were used. Genotoxicity and cytotoxicity were assessed by Ames and MTT tests, respectively. Twelve known compounds including neochlorogenic acid, chlorogenic acid, caffeic acid, isoorientin, p-coumaric acid, isovitexin, ferulic acid, luteolin, aloe-emodin, diosmetin, chrysophanol, and β-sitosterol were identified as the main marker compounds, and terpenoids and condensed tannins were found to be the major class of secondary metabolites of both medicinal plants. The ethyl ether fractions demonstrated the highest antibacterial activity against all the Gram-positive microorganisms, (MIC value of 62 to 1000 µg/mL), with aloe-emodin as one of the main marker compounds highly active against Staphylococcus epidermidis (MIC value of 0.8 to 1.6 µg/mL). Ethyl acetate fractions exhibited the highest antioxidant activity (IC50 of 800 to 1200 µg/mL, respectively). No cytotoxicity (up to 1000 µg/mL) or genotoxicity/mutagenicity (up to 5 mg/plate, with/without metabolic activation) were detected. The obtained results contribute to the knowledge of the value and safety of the studied species as herbal medicines.DQ - Departamento de QuímicaMEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de RecursosRUNMalmir, MaryamLima, KatelenePóvoas Camões, SérgioManageiro, VeraDuarte, Maria PaulaPaiva Miranda, JoanaSerrano, RitaMoreira da Silva, IsabelSilva Lima, BeatrizCaniça, ManuelaSilva, Olga2023-09-27T22:26:44Z2023-03-042023-03-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/158393engMalmir, M., Lima, K., Póvoas Camões, S., Manageiro, V., Duarte, M. P., Paiva Miranda, J., Serrano, R., Moreira da Silva, I., Silva Lima, B., Caniça, M., & Silva, O. (2023). Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts. Molecules, 28(5), [2372]. https://doi.org/10.3390/molecules280523721420-3049PURE: 72622156https://doi.org/10.3390/molecules28052372info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:45Zoai:run.unl.pt:10362/158393Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:07.070195Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| spellingShingle |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts Malmir, Maryam Aloe-emodin antimicrobial antioxidant Asphodelus chemical profile herbal medicines preclinical safety Staphylococcus epidermidis Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry |
| title_short |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_full |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_fullStr |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_full_unstemmed |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| title_sort |
Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts |
| author |
Malmir, Maryam |
| author_facet |
Malmir, Maryam Lima, Katelene Póvoas Camões, Sérgio Manageiro, Vera Duarte, Maria Paula Paiva Miranda, Joana Serrano, Rita Moreira da Silva, Isabel Silva Lima, Beatriz Caniça, Manuela Silva, Olga |
| author_role |
author |
| author2 |
Lima, Katelene Póvoas Camões, Sérgio Manageiro, Vera Duarte, Maria Paula Paiva Miranda, Joana Serrano, Rita Moreira da Silva, Isabel Silva Lima, Beatriz Caniça, Manuela Silva, Olga |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DQ - Departamento de Química MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos RUN |
| dc.contributor.author.fl_str_mv |
Malmir, Maryam Lima, Katelene Póvoas Camões, Sérgio Manageiro, Vera Duarte, Maria Paula Paiva Miranda, Joana Serrano, Rita Moreira da Silva, Isabel Silva Lima, Beatriz Caniça, Manuela Silva, Olga |
| dc.subject.por.fl_str_mv |
Aloe-emodin antimicrobial antioxidant Asphodelus chemical profile herbal medicines preclinical safety Staphylococcus epidermidis Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry |
| topic |
Aloe-emodin antimicrobial antioxidant Asphodelus chemical profile herbal medicines preclinical safety Staphylococcus epidermidis Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry |
| description |
Publisher Copyright: © 2023 by the authors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-09-27T22:26:44Z 2023-03-04 2023-03-04T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/158393 |
| url |
http://hdl.handle.net/10362/158393 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Malmir, M., Lima, K., Póvoas Camões, S., Manageiro, V., Duarte, M. P., Paiva Miranda, J., Serrano, R., Moreira da Silva, I., Silva Lima, B., Caniça, M., & Silva, O. (2023). Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts. Molecules, 28(5), [2372]. https://doi.org/10.3390/molecules28052372 1420-3049 PURE: 72622156 https://doi.org/10.3390/molecules28052372 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
18 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138154488266752 |