The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate

Detalhes bibliográficos
Autor(a) principal: Alves, R.
Data de Publicação: 2017
Outros Autores: Mota, Sandra, Silva, Sónia Carina, Rodrigues, Célia F., Brown, Alistair J., Henriques, Mariana, Casal, Margarida, Paiva, Sandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/48442
Resumo: Candida albicans has the ability to adapt to different host niches, often glucose-limited but rich in alternative carbon sources. In these glucose-poor microenvironments, this pathogen expresses JEN1 and JEN2 genes, encoding carboxylate transporters, which are important in the early stages of infection. This work investigated how host microenvironments, in particular acidic containing lactic acid, affect C. albicans biofilm formation and antifungal drug resistance. Multiple components of the extracellular matrix were also analysed, including their impact on antifungal drug resistance, and the involvement of both Jen1 and Jen2 in this process. The results show that growth on lactate affects biofilm formation, morphology and susceptibility to fluconazole and that both Jen1 and Jen2 might play a role in these processes. These results support the view that the adaptation of Candida cells to the carbon source present in the host niches affects their pathogenicity.
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spelling The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactateCandidaCandida albicansFluconazoleCarboxylic acid transportersJen1 Jen2BiofilmMatrixResistancealternative carbon sourcesantifungal drug resistancebiofilm formationlactateScience & TechnologyCandida albicans has the ability to adapt to different host niches, often glucose-limited but rich in alternative carbon sources. In these glucose-poor microenvironments, this pathogen expresses JEN1 and JEN2 genes, encoding carboxylate transporters, which are important in the early stages of infection. This work investigated how host microenvironments, in particular acidic containing lactic acid, affect C. albicans biofilm formation and antifungal drug resistance. Multiple components of the extracellular matrix were also analysed, including their impact on antifungal drug resistance, and the involvement of both Jen1 and Jen2 in this process. The results show that growth on lactate affects biofilm formation, morphology and susceptibility to fluconazole and that both Jen1 and Jen2 might play a role in these processes. These results support the view that the adaptation of Candida cells to the carbon source present in the host niches affects their pathogenicity.This study was funded by the Portuguese Foundation for Science and Technology (FCT) [grant number PTDC/BIAMIC/5184/2014]. SM, CFR and RA received FCT PhD studentships [grant numbers SFRH/BD/74790/2010, SFRH/ BD/93078/2013, PD/BD/113813/2015, respectively]. The work on CBMA was supported by FCT [grant number UID/ BIA/04050/2013] and COMPETE 2020 [grant number POCI01-0145-FEDER-007569]. The work on CEB was supported by FCT [grant number UID/BIO/04469/2013], COMPETE 2020 [grant number POCI-01-0145-FEDER-006684] and BioTecNorte operation [grant number NORTE-01-0145FEDER-000004] funded by the ERDF under the scope of Norte2020 – Programa Operacional Regional do Norte. AJPB was funded by the UK Medical Research Council [grant number MR/M026663/1]; by the UK Biotechnology and Biological Research Council [grant number BB/K017365/1]; and by the MRC Centre for Medical Mycology and the University of Aberdeen [grant number MR/M026663/1]. The funders had no role in study design, data collection and analysis, the decision to publish, or in the preparation of the manuscript.info:eu-repo/semantics/publishedVersionTaylor and FrancisUniversidade do MinhoAlves, R.Mota, SandraSilva, Sónia CarinaRodrigues, Célia F.Brown, Alistair J.Henriques, MarianaCasal, MargaridaPaiva, Sandra2017-11-022017-11-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/48442engAlves, R.; Mota, Sandra; Silva, Sónia Carina; Rodrigues, Célia F.; Brown, Alistair J.; Henriques, Mariana; Casal, Margarida; Paiva, Sandra, The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate. Biofouling - The Journal of Bioadhesion and Biofilm Research, 33(10), 943-954, 2017089270141029-245410.1080/08927014.2017.139251429094611http://www.tandfonline.com/action/journalInformation?journalCode=gbif20info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:46Zoai:repositorium.sdum.uminho.pt:1822/48442Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:38:50.470798Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
title The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
spellingShingle The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
Alves, R.
Candida
Candida albicans
Fluconazole
Carboxylic acid transporters
Jen1 Jen2
Biofilm
Matrix
Resistance
alternative carbon sources
antifungal drug resistance
biofilm formation
lactate
Science & Technology
title_short The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
title_full The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
title_fullStr The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
title_full_unstemmed The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
title_sort The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate
author Alves, R.
author_facet Alves, R.
Mota, Sandra
Silva, Sónia Carina
Rodrigues, Célia F.
Brown, Alistair J.
Henriques, Mariana
Casal, Margarida
Paiva, Sandra
author_role author
author2 Mota, Sandra
Silva, Sónia Carina
Rodrigues, Célia F.
Brown, Alistair J.
Henriques, Mariana
Casal, Margarida
Paiva, Sandra
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Alves, R.
Mota, Sandra
Silva, Sónia Carina
Rodrigues, Célia F.
Brown, Alistair J.
Henriques, Mariana
Casal, Margarida
Paiva, Sandra
dc.subject.por.fl_str_mv Candida
Candida albicans
Fluconazole
Carboxylic acid transporters
Jen1 Jen2
Biofilm
Matrix
Resistance
alternative carbon sources
antifungal drug resistance
biofilm formation
lactate
Science & Technology
topic Candida
Candida albicans
Fluconazole
Carboxylic acid transporters
Jen1 Jen2
Biofilm
Matrix
Resistance
alternative carbon sources
antifungal drug resistance
biofilm formation
lactate
Science & Technology
description Candida albicans has the ability to adapt to different host niches, often glucose-limited but rich in alternative carbon sources. In these glucose-poor microenvironments, this pathogen expresses JEN1 and JEN2 genes, encoding carboxylate transporters, which are important in the early stages of infection. This work investigated how host microenvironments, in particular acidic containing lactic acid, affect C. albicans biofilm formation and antifungal drug resistance. Multiple components of the extracellular matrix were also analysed, including their impact on antifungal drug resistance, and the involvement of both Jen1 and Jen2 in this process. The results show that growth on lactate affects biofilm formation, morphology and susceptibility to fluconazole and that both Jen1 and Jen2 might play a role in these processes. These results support the view that the adaptation of Candida cells to the carbon source present in the host niches affects their pathogenicity.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-02
2017-11-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/48442
url http://hdl.handle.net/1822/48442
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, R.; Mota, Sandra; Silva, Sónia Carina; Rodrigues, Célia F.; Brown, Alistair J.; Henriques, Mariana; Casal, Margarida; Paiva, Sandra, The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate. Biofouling - The Journal of Bioadhesion and Biofilm Research, 33(10), 943-954, 2017
08927014
1029-2454
10.1080/08927014.2017.1392514
29094611
http://www.tandfonline.com/action/journalInformation?journalCode=gbif20
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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