Modification of microfiltration membranes by hydrogel impregnation for pDNA purification
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4665 |
Resumo: | The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane‐based technologies are the best match to assure simplicity, efficiency and easy scale‐up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR‐FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses. |
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Modification of microfiltration membranes by hydrogel impregnation for pDNA purificationMembranesNucleic acidsPolysaccharidesThe huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane‐based technologies are the best match to assure simplicity, efficiency and easy scale‐up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR‐FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses.WileyuBibliorumCastilho, Pedro Henrique BarataCorreia, Tiago R.Amorim, Maria T. Pessoa deEscobar, IsabelQueiroz, JoãoCorreia, Ilídio Joaquim SobreiraMorão, António2018-03-20T15:33:51Z2015-03-022015-03-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4665engCastilho, P.H., Correia, T.R., Amorim, M.T.P., Escobar, I.C., Queiroz, J.A., Correia, I.J. e Morão A.M. (2015) “Modification of microfiltration membranes by hydrogel impregnation for pDNA purification", Journal of Applied Polymer Science, Vol. 132(21): pp. 4161010.1002/app.41610metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:41:52Zoai:ubibliorum.ubi.pt:10400.6/4665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:43.364616Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
spellingShingle |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification Castilho, Pedro Henrique Barata Membranes Nucleic acids Polysaccharides |
title_short |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_full |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_fullStr |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_full_unstemmed |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_sort |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
author |
Castilho, Pedro Henrique Barata |
author_facet |
Castilho, Pedro Henrique Barata Correia, Tiago R. Amorim, Maria T. Pessoa de Escobar, Isabel Queiroz, João Correia, Ilídio Joaquim Sobreira Morão, António |
author_role |
author |
author2 |
Correia, Tiago R. Amorim, Maria T. Pessoa de Escobar, Isabel Queiroz, João Correia, Ilídio Joaquim Sobreira Morão, António |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Castilho, Pedro Henrique Barata Correia, Tiago R. Amorim, Maria T. Pessoa de Escobar, Isabel Queiroz, João Correia, Ilídio Joaquim Sobreira Morão, António |
dc.subject.por.fl_str_mv |
Membranes Nucleic acids Polysaccharides |
topic |
Membranes Nucleic acids Polysaccharides |
description |
The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane‐based technologies are the best match to assure simplicity, efficiency and easy scale‐up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR‐FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-02 2015-03-02T00:00:00Z 2018-03-20T15:33:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4665 |
url |
http://hdl.handle.net/10400.6/4665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Castilho, P.H., Correia, T.R., Amorim, M.T.P., Escobar, I.C., Queiroz, J.A., Correia, I.J. e Morão A.M. (2015) “Modification of microfiltration membranes by hydrogel impregnation for pDNA purification", Journal of Applied Polymer Science, Vol. 132(21): pp. 41610 10.1002/app.41610 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136354480685056 |