Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/26504 |
Resumo: | Cell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight proteins, Fig. 3 into the cell cytoplasm and organelles. The development of CPPs as transduction agents includes the modi fi cation of gene and protein expression, the reprogramming and di ff erentiation of induced pluripotent stem cells and the preparation of cellular vaccines. A relatively recent fi eld of CPP application is the transduction of plasmid DNA vectors and CPP-fusion proteins to modify genomes and introduce new traits in cells and organisms. CPP-mediated transduction of components for genome editing is an advantageous alternative to viral DNA vectors. Engineered site-speci fi c nucleases, such as Cre recombinase, ZFN, TALENs and CRISPR associated protein (Cas), have been coupled to CPPs, and the fused proteins have been used to permeate targeted cells and tissues. The functionally active fusion CPP-nucleases subsequently home to the nucleus, incise genomic DNA at speci fi c sites and induce repair and recombination. This review has the objective of discussing CPPs and elucidating the prospective use of CPP-mediated transduction technology, particularly in genome modification and transgenesis |
id |
UFC-7_bb7ddaf4a36cdc72a9d91eef8f56b824 |
---|---|
oai_identifier_str |
oai:repositorio.ufc.br:riufc/26504 |
network_acronym_str |
UFC-7 |
network_name_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository_id_str |
|
spelling |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesisÁcidos NucleicosNucleic AcidsCell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight proteins, Fig. 3 into the cell cytoplasm and organelles. The development of CPPs as transduction agents includes the modi fi cation of gene and protein expression, the reprogramming and di ff erentiation of induced pluripotent stem cells and the preparation of cellular vaccines. A relatively recent fi eld of CPP application is the transduction of plasmid DNA vectors and CPP-fusion proteins to modify genomes and introduce new traits in cells and organisms. CPP-mediated transduction of components for genome editing is an advantageous alternative to viral DNA vectors. Engineered site-speci fi c nucleases, such as Cre recombinase, ZFN, TALENs and CRISPR associated protein (Cas), have been coupled to CPPs, and the fused proteins have been used to permeate targeted cells and tissues. The functionally active fusion CPP-nucleases subsequently home to the nucleus, incise genomic DNA at speci fi c sites and induce repair and recombination. This review has the objective of discussing CPPs and elucidating the prospective use of CPP-mediated transduction technology, particularly in genome modification and transgenesisJournal of Biotechnology2017-10-09T18:36:53Z2017-10-09T18:36:53Z2017-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRÁDIS-BAPTISTA, G. R. et al. Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. Journal of Biotechnology, Amsterdam, v. 252, p. 15-26, jun. 2017.0168-1656http://www.repositorio.ufc.br/handle/riufc/26504Rádis-Baptista, GandhiCampelo, Iana S.Morlighem, Jean-Étienne R. L.Melo, Luciana M.Freitas, Vicente J. F. Freitasengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-25T15:05:50Zoai:repositorio.ufc.br:riufc/26504Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:18:56.862928Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
title |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
spellingShingle |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis Rádis-Baptista, Gandhi Ácidos Nucleicos Nucleic Acids |
title_short |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
title_full |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
title_fullStr |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
title_full_unstemmed |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
title_sort |
Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis |
author |
Rádis-Baptista, Gandhi |
author_facet |
Rádis-Baptista, Gandhi Campelo, Iana S. Morlighem, Jean-Étienne R. L. Melo, Luciana M. Freitas, Vicente J. F. Freitas |
author_role |
author |
author2 |
Campelo, Iana S. Morlighem, Jean-Étienne R. L. Melo, Luciana M. Freitas, Vicente J. F. Freitas |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rádis-Baptista, Gandhi Campelo, Iana S. Morlighem, Jean-Étienne R. L. Melo, Luciana M. Freitas, Vicente J. F. Freitas |
dc.subject.por.fl_str_mv |
Ácidos Nucleicos Nucleic Acids |
topic |
Ácidos Nucleicos Nucleic Acids |
description |
Cell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight proteins, Fig. 3 into the cell cytoplasm and organelles. The development of CPPs as transduction agents includes the modi fi cation of gene and protein expression, the reprogramming and di ff erentiation of induced pluripotent stem cells and the preparation of cellular vaccines. A relatively recent fi eld of CPP application is the transduction of plasmid DNA vectors and CPP-fusion proteins to modify genomes and introduce new traits in cells and organisms. CPP-mediated transduction of components for genome editing is an advantageous alternative to viral DNA vectors. Engineered site-speci fi c nucleases, such as Cre recombinase, ZFN, TALENs and CRISPR associated protein (Cas), have been coupled to CPPs, and the fused proteins have been used to permeate targeted cells and tissues. The functionally active fusion CPP-nucleases subsequently home to the nucleus, incise genomic DNA at speci fi c sites and induce repair and recombination. This review has the objective of discussing CPPs and elucidating the prospective use of CPP-mediated transduction technology, particularly in genome modification and transgenesis |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-09T18:36:53Z 2017-10-09T18:36:53Z 2017-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
RÁDIS-BAPTISTA, G. R. et al. Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. Journal of Biotechnology, Amsterdam, v. 252, p. 15-26, jun. 2017. 0168-1656 http://www.repositorio.ufc.br/handle/riufc/26504 |
identifier_str_mv |
RÁDIS-BAPTISTA, G. R. et al. Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. Journal of Biotechnology, Amsterdam, v. 252, p. 15-26, jun. 2017. 0168-1656 |
url |
http://www.repositorio.ufc.br/handle/riufc/26504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Biotechnology |
publisher.none.fl_str_mv |
Journal of Biotechnology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1813028751280177152 |