Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis

Detalhes bibliográficos
Autor(a) principal: Rádis-Baptista, Gandhi
Data de Publicação: 2017
Outros Autores: Campelo, Iana S., Morlighem, Jean-Étienne R. L., Melo, Luciana M., Freitas, Vicente J. F. Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/26504
Resumo: Cell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight proteins, Fig. 3 into the cell cytoplasm and organelles. The development of CPPs as transduction agents includes the modi fi cation of gene and protein expression, the reprogramming and di ff erentiation of induced pluripotent stem cells and the preparation of cellular vaccines. A relatively recent fi eld of CPP application is the transduction of plasmid DNA vectors and CPP-fusion proteins to modify genomes and introduce new traits in cells and organisms. CPP-mediated transduction of components for genome editing is an advantageous alternative to viral DNA vectors. Engineered site-speci fi c nucleases, such as Cre recombinase, ZFN, TALENs and CRISPR associated protein (Cas), have been coupled to CPPs, and the fused proteins have been used to permeate targeted cells and tissues. The functionally active fusion CPP-nucleases subsequently home to the nucleus, incise genomic DNA at speci fi c sites and induce repair and recombination. This review has the objective of discussing CPPs and elucidating the prospective use of CPP-mediated transduction technology, particularly in genome modification and transgenesis
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spelling Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesisÁcidos NucleicosNucleic AcidsCell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight proteins, Fig. 3 into the cell cytoplasm and organelles. The development of CPPs as transduction agents includes the modi fi cation of gene and protein expression, the reprogramming and di ff erentiation of induced pluripotent stem cells and the preparation of cellular vaccines. A relatively recent fi eld of CPP application is the transduction of plasmid DNA vectors and CPP-fusion proteins to modify genomes and introduce new traits in cells and organisms. CPP-mediated transduction of components for genome editing is an advantageous alternative to viral DNA vectors. Engineered site-speci fi c nucleases, such as Cre recombinase, ZFN, TALENs and CRISPR associated protein (Cas), have been coupled to CPPs, and the fused proteins have been used to permeate targeted cells and tissues. The functionally active fusion CPP-nucleases subsequently home to the nucleus, incise genomic DNA at speci fi c sites and induce repair and recombination. This review has the objective of discussing CPPs and elucidating the prospective use of CPP-mediated transduction technology, particularly in genome modification and transgenesisJournal of Biotechnology2017-10-09T18:36:53Z2017-10-09T18:36:53Z2017-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRÁDIS-BAPTISTA, G. R. et al. Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. Journal of Biotechnology, Amsterdam, v. 252, p. 15-26, jun. 2017.0168-1656http://www.repositorio.ufc.br/handle/riufc/26504Rádis-Baptista, GandhiCampelo, Iana S.Morlighem, Jean-Étienne R. L.Melo, Luciana M.Freitas, Vicente J. F. Freitasengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-25T15:05:50Zoai:repositorio.ufc.br:riufc/26504Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:18:56.862928Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
title Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
spellingShingle Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
Rádis-Baptista, Gandhi
Ácidos Nucleicos
Nucleic Acids
title_short Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
title_full Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
title_fullStr Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
title_full_unstemmed Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
title_sort Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis
author Rádis-Baptista, Gandhi
author_facet Rádis-Baptista, Gandhi
Campelo, Iana S.
Morlighem, Jean-Étienne R. L.
Melo, Luciana M.
Freitas, Vicente J. F. Freitas
author_role author
author2 Campelo, Iana S.
Morlighem, Jean-Étienne R. L.
Melo, Luciana M.
Freitas, Vicente J. F. Freitas
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rádis-Baptista, Gandhi
Campelo, Iana S.
Morlighem, Jean-Étienne R. L.
Melo, Luciana M.
Freitas, Vicente J. F. Freitas
dc.subject.por.fl_str_mv Ácidos Nucleicos
Nucleic Acids
topic Ácidos Nucleicos
Nucleic Acids
description Cell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight proteins, Fig. 3 into the cell cytoplasm and organelles. The development of CPPs as transduction agents includes the modi fi cation of gene and protein expression, the reprogramming and di ff erentiation of induced pluripotent stem cells and the preparation of cellular vaccines. A relatively recent fi eld of CPP application is the transduction of plasmid DNA vectors and CPP-fusion proteins to modify genomes and introduce new traits in cells and organisms. CPP-mediated transduction of components for genome editing is an advantageous alternative to viral DNA vectors. Engineered site-speci fi c nucleases, such as Cre recombinase, ZFN, TALENs and CRISPR associated protein (Cas), have been coupled to CPPs, and the fused proteins have been used to permeate targeted cells and tissues. The functionally active fusion CPP-nucleases subsequently home to the nucleus, incise genomic DNA at speci fi c sites and induce repair and recombination. This review has the objective of discussing CPPs and elucidating the prospective use of CPP-mediated transduction technology, particularly in genome modification and transgenesis
publishDate 2017
dc.date.none.fl_str_mv 2017-10-09T18:36:53Z
2017-10-09T18:36:53Z
2017-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RÁDIS-BAPTISTA, G. R. et al. Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. Journal of Biotechnology, Amsterdam, v. 252, p. 15-26, jun. 2017.
0168-1656
http://www.repositorio.ufc.br/handle/riufc/26504
identifier_str_mv RÁDIS-BAPTISTA, G. R. et al. Cell-penetrating peptides (CPPs): from delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis. Journal of Biotechnology, Amsterdam, v. 252, p. 15-26, jun. 2017.
0168-1656
url http://www.repositorio.ufc.br/handle/riufc/26504
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Journal of Biotechnology
publisher.none.fl_str_mv Journal of Biotechnology
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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