Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering

Detalhes bibliográficos
Autor(a) principal: Rocha, Pedro M.
Data de Publicação: 2011
Outros Autores: Espírito Santo, Vítor, Gomes, Manuela E., Reis, R. L., Mano, J. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/14277
Resumo: Tissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-b1 (TGF-b1). The ATDC5 cell line was encapsulated to determine the cytotoxicity and the influence of polymer concentration on cell viability and proliferation. Human adipose-derived stem cells (hASCs) were encapsulated with TGF-b1 in the hydrogel networks to enhance the chondrogenic differentiation of hASCs. Specific cartilage extracellular matrix molecules expression by hASCs were observed after 14 days of cultures of the constructs under different conditions. The k-carrageenan was found to be a suitable biomaterial for cell and GF encapsulation. The incorporation of TGF-b1 within the carrageenan-based hydrogel enhanced the cartilage differentiation of hASCs. These findings indicate that this new system for cartilage TE is very promising for injectable thermoresponsive formulation applications.
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spelling Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineeringCarrageenanAdipose derived stem cellsHydrogelCartilage tissue engineeringTransforming growth factorControlled releaseScience & TechnologyTissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-b1 (TGF-b1). The ATDC5 cell line was encapsulated to determine the cytotoxicity and the influence of polymer concentration on cell viability and proliferation. Human adipose-derived stem cells (hASCs) were encapsulated with TGF-b1 in the hydrogel networks to enhance the chondrogenic differentiation of hASCs. Specific cartilage extracellular matrix molecules expression by hASCs were observed after 14 days of cultures of the constructs under different conditions. The k-carrageenan was found to be a suitable biomaterial for cell and GF encapsulation. The incorporation of TGF-b1 within the carrageenan-based hydrogel enhanced the cartilage differentiation of hASCs. These findings indicate that this new system for cartilage TE is very promising for injectable thermoresponsive formulation applications.This study was supported by the European NoE EXPERTISSUES (NMP3-CT-2004-500283), European FP7 Project Find and Bind (NMP4-SL-2009-229292), and by the Portuguese Foundation for Science and Technology (FCT) through the projects PTDC/FIS/68517/2006, PTDC/QUI/69263/2006, and through V. E. Santo's PhD grant (BD/39486/2007). They are also grateful to Hospital da Prelada for the lipoaspirates donations, especially to Doctor Paulo Costa.SAGEUniversidade do MinhoRocha, Pedro M.Espírito Santo, VítorGomes, Manuela E.Reis, R. L.Mano, J. F.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/14277eng0883-911510.1177/0883911511420700info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:24Zoai:repositorium.sdum.uminho.pt:1822/14277Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:18.287046Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
title Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
spellingShingle Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
Rocha, Pedro M.
Carrageenan
Adipose derived stem cells
Hydrogel
Cartilage tissue engineering
Transforming growth factor
Controlled release
Science & Technology
title_short Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
title_full Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
title_fullStr Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
title_full_unstemmed Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
title_sort Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering
author Rocha, Pedro M.
author_facet Rocha, Pedro M.
Espírito Santo, Vítor
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
author_role author
author2 Espírito Santo, Vítor
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rocha, Pedro M.
Espírito Santo, Vítor
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
dc.subject.por.fl_str_mv Carrageenan
Adipose derived stem cells
Hydrogel
Cartilage tissue engineering
Transforming growth factor
Controlled release
Science & Technology
topic Carrageenan
Adipose derived stem cells
Hydrogel
Cartilage tissue engineering
Transforming growth factor
Controlled release
Science & Technology
description Tissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-b1 (TGF-b1). The ATDC5 cell line was encapsulated to determine the cytotoxicity and the influence of polymer concentration on cell viability and proliferation. Human adipose-derived stem cells (hASCs) were encapsulated with TGF-b1 in the hydrogel networks to enhance the chondrogenic differentiation of hASCs. Specific cartilage extracellular matrix molecules expression by hASCs were observed after 14 days of cultures of the constructs under different conditions. The k-carrageenan was found to be a suitable biomaterial for cell and GF encapsulation. The incorporation of TGF-b1 within the carrageenan-based hydrogel enhanced the cartilage differentiation of hASCs. These findings indicate that this new system for cartilage TE is very promising for injectable thermoresponsive formulation applications.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
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url http://hdl.handle.net/1822/14277
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 0883-9115
10.1177/0883911511420700
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dc.publisher.none.fl_str_mv SAGE
publisher.none.fl_str_mv SAGE
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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