Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4701 |
Resumo: | Electricity production in microbial fuel cells (MFCs) is an emerging green alternative to the use of fossil fuels. Shewanella oneidensis MR‐1 (SOMR‐1) is a Gram‐negative bacterium, adapted to MFCs due to its ability to link its bioenergetic metabolism through the periplasm to reduce extracellular electron acceptors. OmcA is a highly abundant outer‐membrane cytochrome of SOMR‐1 cells and is involved in the extracellular electron transfer to solid acceptors and electron shuttles. To investigate electron transfer performed by OmcA towards final acceptors, site directed mutagenesis was used to disturb the axial coordination of hemes. Interactions between OmcA and redox partners such as iron and graphene oxides, and electron shuttles were characterized using nuclear magnetic resonance and stopped‐flow experiments. Results showed that solid electron acceptors do not come into close proximity to the hemes, in agreement with experimentally observed slow electron transfer. In contrast, mutation of the distal axial ligand of heme VII changes the driving force of OmcA towards electron shuttles and reduces the affinity of the FMN:OmcA complex. Overall, these results reveal a functional specificity of particular hemes of OmcA and provide guidance for the rational design of mutated SOMR‐1 strains optimized for operating in different microbial electrochemical devices. |
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Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode InterfaceExtracellular Electron TransferFlavinsGrapheneOuter membrane CytochromesShewanella oneidensis MR-1Electricity production in microbial fuel cells (MFCs) is an emerging green alternative to the use of fossil fuels. Shewanella oneidensis MR‐1 (SOMR‐1) is a Gram‐negative bacterium, adapted to MFCs due to its ability to link its bioenergetic metabolism through the periplasm to reduce extracellular electron acceptors. OmcA is a highly abundant outer‐membrane cytochrome of SOMR‐1 cells and is involved in the extracellular electron transfer to solid acceptors and electron shuttles. To investigate electron transfer performed by OmcA towards final acceptors, site directed mutagenesis was used to disturb the axial coordination of hemes. Interactions between OmcA and redox partners such as iron and graphene oxides, and electron shuttles were characterized using nuclear magnetic resonance and stopped‐flow experiments. Results showed that solid electron acceptors do not come into close proximity to the hemes, in agreement with experimentally observed slow electron transfer. In contrast, mutation of the distal axial ligand of heme VII changes the driving force of OmcA towards electron shuttles and reduces the affinity of the FMN:OmcA complex. Overall, these results reveal a functional specificity of particular hemes of OmcA and provide guidance for the rational design of mutated SOMR‐1 strains optimized for operating in different microbial electrochemical devices.WileyuBibliorumNeto, Sónia de Fátima EstevãoDiogo, Duarte Miguel de MeloCorreia, I.J.Paquete, CatarinaLouro, Ricardo2018-03-22T09:21:54Z2017-07-252017-07-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4701engNeto, S. E., Melo‐Diogo, D., Correia, I. J., Paquete, C. M., & Louro, R. O. (2017). “Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface.” Fuel Cells, Vol.17 (5), pp.601-61110.1002/fuce.201700023metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:42:00Zoai:ubibliorum.ubi.pt:10400.6/4701Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:45.154956Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
title |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
spellingShingle |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface Neto, Sónia de Fátima Estevão Extracellular Electron Transfer Flavins Graphene Outer membrane Cytochromes Shewanella oneidensis MR-1 |
title_short |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
title_full |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
title_fullStr |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
title_full_unstemmed |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
title_sort |
Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface |
author |
Neto, Sónia de Fátima Estevão |
author_facet |
Neto, Sónia de Fátima Estevão Diogo, Duarte Miguel de Melo Correia, I.J. Paquete, Catarina Louro, Ricardo |
author_role |
author |
author2 |
Diogo, Duarte Miguel de Melo Correia, I.J. Paquete, Catarina Louro, Ricardo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Neto, Sónia de Fátima Estevão Diogo, Duarte Miguel de Melo Correia, I.J. Paquete, Catarina Louro, Ricardo |
dc.subject.por.fl_str_mv |
Extracellular Electron Transfer Flavins Graphene Outer membrane Cytochromes Shewanella oneidensis MR-1 |
topic |
Extracellular Electron Transfer Flavins Graphene Outer membrane Cytochromes Shewanella oneidensis MR-1 |
description |
Electricity production in microbial fuel cells (MFCs) is an emerging green alternative to the use of fossil fuels. Shewanella oneidensis MR‐1 (SOMR‐1) is a Gram‐negative bacterium, adapted to MFCs due to its ability to link its bioenergetic metabolism through the periplasm to reduce extracellular electron acceptors. OmcA is a highly abundant outer‐membrane cytochrome of SOMR‐1 cells and is involved in the extracellular electron transfer to solid acceptors and electron shuttles. To investigate electron transfer performed by OmcA towards final acceptors, site directed mutagenesis was used to disturb the axial coordination of hemes. Interactions between OmcA and redox partners such as iron and graphene oxides, and electron shuttles were characterized using nuclear magnetic resonance and stopped‐flow experiments. Results showed that solid electron acceptors do not come into close proximity to the hemes, in agreement with experimentally observed slow electron transfer. In contrast, mutation of the distal axial ligand of heme VII changes the driving force of OmcA towards electron shuttles and reduces the affinity of the FMN:OmcA complex. Overall, these results reveal a functional specificity of particular hemes of OmcA and provide guidance for the rational design of mutated SOMR‐1 strains optimized for operating in different microbial electrochemical devices. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-25 2017-07-25T00:00:00Z 2018-03-22T09:21:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4701 |
url |
http://hdl.handle.net/10400.6/4701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neto, S. E., Melo‐Diogo, D., Correia, I. J., Paquete, C. M., & Louro, R. O. (2017). “Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface.” Fuel Cells, Vol.17 (5), pp.601-611 10.1002/fuce.201700023 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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