Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese

Detalhes bibliográficos
Autor(a) principal: Amador, Paula
Data de Publicação: 2009
Outros Autores: Fernandes, Rúben, Prudêncio, Cristina, Brito, Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/1982
Resumo: The purpose of this study was to investigate the presence of beta-lactam-resistant bacteria in six different types of Portuguese cheese. The numbers of ampicillin resistant (AMP(r)) bacteria varied from 4.7 x 10(2) to 1.5 x 10(7) CFU/g. Within 172 randomly selected beta-lactam-resistant bacteria, 44 resistant phenotypes were found and 31.4% were multidrug resistant. The majority (85%) of the isolates identified belonged to the Enterobacteriaceae family. The presence of the bla(TEM) gene was detected in 80.9% of the tested isolates. The results suggest that without thermal processing of the milk and good hygienic practices, cheese may act as a vehicle of transfer of beta-lactam-resistant bacteria to the gastrointestinal tract of consumers.
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spelling Resistance to β-lactams in bacteria isolated from different types of Portuguese cheeseReady-to-eat (RTE) foodCheese, minimal inhibitory concentration (MIC)β-lactam-resistant enterobacteriaceaeMultiplex-PCRBlaTEM geneThe purpose of this study was to investigate the presence of beta-lactam-resistant bacteria in six different types of Portuguese cheese. The numbers of ampicillin resistant (AMP(r)) bacteria varied from 4.7 x 10(2) to 1.5 x 10(7) CFU/g. Within 172 randomly selected beta-lactam-resistant bacteria, 44 resistant phenotypes were found and 31.4% were multidrug resistant. The majority (85%) of the isolates identified belonged to the Enterobacteriaceae family. The presence of the bla(TEM) gene was detected in 80.9% of the tested isolates. The results suggest that without thermal processing of the milk and good hygienic practices, cheese may act as a vehicle of transfer of beta-lactam-resistant bacteria to the gastrointestinal tract of consumers.MDPIRepositório Científico do Instituto Politécnico do PortoAmador, PaulaFernandes, RúbenPrudêncio, CristinaBrito, Luísa2013-09-20T14:19:57Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/1982eng1422-006710.3390/ijms10041538info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:41:09Zoai:recipp.ipp.pt:10400.22/1982Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:22:51.952481Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
title Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
spellingShingle Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
Amador, Paula
Ready-to-eat (RTE) food
Cheese, minimal inhibitory concentration (MIC)
β-lactam-resistant enterobacteriaceae
Multiplex-PCR
BlaTEM gene
title_short Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
title_full Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
title_fullStr Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
title_full_unstemmed Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
title_sort Resistance to β-lactams in bacteria isolated from different types of Portuguese cheese
author Amador, Paula
author_facet Amador, Paula
Fernandes, Rúben
Prudêncio, Cristina
Brito, Luísa
author_role author
author2 Fernandes, Rúben
Prudêncio, Cristina
Brito, Luísa
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Amador, Paula
Fernandes, Rúben
Prudêncio, Cristina
Brito, Luísa
dc.subject.por.fl_str_mv Ready-to-eat (RTE) food
Cheese, minimal inhibitory concentration (MIC)
β-lactam-resistant enterobacteriaceae
Multiplex-PCR
BlaTEM gene
topic Ready-to-eat (RTE) food
Cheese, minimal inhibitory concentration (MIC)
β-lactam-resistant enterobacteriaceae
Multiplex-PCR
BlaTEM gene
description The purpose of this study was to investigate the presence of beta-lactam-resistant bacteria in six different types of Portuguese cheese. The numbers of ampicillin resistant (AMP(r)) bacteria varied from 4.7 x 10(2) to 1.5 x 10(7) CFU/g. Within 172 randomly selected beta-lactam-resistant bacteria, 44 resistant phenotypes were found and 31.4% were multidrug resistant. The majority (85%) of the isolates identified belonged to the Enterobacteriaceae family. The presence of the bla(TEM) gene was detected in 80.9% of the tested isolates. The results suggest that without thermal processing of the milk and good hygienic practices, cheese may act as a vehicle of transfer of beta-lactam-resistant bacteria to the gastrointestinal tract of consumers.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2013-09-20T14:19:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/1982
url http://hdl.handle.net/10400.22/1982
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1422-0067
10.3390/ijms10041538
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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