Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride

Detalhes bibliográficos
Autor(a) principal: Machado, Idalina
Data de Publicação: 2013
Outros Autores: Coquet, Laurent, Jouenne, Thierry, Pereira, Maria Olívia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25524
Resumo: This study aimed to assess the membrane modifications in Pseudomonas aeruginosa after continuous exposure to increasing doses of benzalkonium chloride (BC). Two different concentrations were used, 0.9 and 12.0 mM. Proteomic investigations revealed that the range of the outermembrane proteome alterations following continuous exposure is very low, i.e. about 10% and BC concentration dependent. Adapted cells revealed different expressions of key proteins frequently reported as involved in acquired resistance mechanisms. Porins (OprF and OprG) and lipoproteins (OprL and OprI) were underexpressed when the higher adaptation concentration (12 mM) was used. Some of thesemembrane alterations have been described as involved in the acquired resistance to antibiotics, suggesting possible commonmechanisms between these two types of resistance.
id RCAP_4973438cfbd330cf5c4fa19a2a9de825
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/25524
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chlorideP. aeruginosaProteomeChemical adaptationScience & TechnologyThis study aimed to assess the membrane modifications in Pseudomonas aeruginosa after continuous exposure to increasing doses of benzalkonium chloride (BC). Two different concentrations were used, 0.9 and 12.0 mM. Proteomic investigations revealed that the range of the outermembrane proteome alterations following continuous exposure is very low, i.e. about 10% and BC concentration dependent. Adapted cells revealed different expressions of key proteins frequently reported as involved in acquired resistance mechanisms. Porins (OprF and OprG) and lipoproteins (OprL and OprI) were underexpressed when the higher adaptation concentration (12 mM) was used. Some of thesemembrane alterations have been described as involved in the acquired resistance to antibiotics, suggesting possible commonmechanisms between these two types of resistance.The authors would like to acknowledge the financial support from IBB-CEB and Fundacao para a Ciencia e Tecnologia (FCT) and European Community fund FEDER, through Program COMPETE, in the ambit of the Project PTDC/SAUESA/64609/2006/FCOMP-01-0124-FEDER-00702 and Idalina Machado PhD Grant (SFRH/BD/31065/2006).ElsevierElsevier BVUniversidade do MinhoMachado, IdalinaCoquet, LaurentJouenne, ThierryPereira, Maria Olívia20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25524eng1874-39191874-391910.1016/j.jprot.2013.04.03023651563www.elsevier.com/locate/jprotinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:18Zoai:repositorium.sdum.uminho.pt:1822/25524Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:47.114895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
title Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
spellingShingle Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
Machado, Idalina
P. aeruginosa
Proteome
Chemical adaptation
Science & Technology
title_short Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
title_full Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
title_fullStr Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
title_full_unstemmed Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
title_sort Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkonium chloride
author Machado, Idalina
author_facet Machado, Idalina
Coquet, Laurent
Jouenne, Thierry
Pereira, Maria Olívia
author_role author
author2 Coquet, Laurent
Jouenne, Thierry
Pereira, Maria Olívia
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Machado, Idalina
Coquet, Laurent
Jouenne, Thierry
Pereira, Maria Olívia
dc.subject.por.fl_str_mv P. aeruginosa
Proteome
Chemical adaptation
Science & Technology
topic P. aeruginosa
Proteome
Chemical adaptation
Science & Technology
description This study aimed to assess the membrane modifications in Pseudomonas aeruginosa after continuous exposure to increasing doses of benzalkonium chloride (BC). Two different concentrations were used, 0.9 and 12.0 mM. Proteomic investigations revealed that the range of the outermembrane proteome alterations following continuous exposure is very low, i.e. about 10% and BC concentration dependent. Adapted cells revealed different expressions of key proteins frequently reported as involved in acquired resistance mechanisms. Porins (OprF and OprG) and lipoproteins (OprL and OprI) were underexpressed when the higher adaptation concentration (12 mM) was used. Some of thesemembrane alterations have been described as involved in the acquired resistance to antibiotics, suggesting possible commonmechanisms between these two types of resistance.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/25524
url http://hdl.handle.net/1822/25524
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1874-3919
1874-3919
10.1016/j.jprot.2013.04.030
23651563
www.elsevier.com/locate/jprot
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
Elsevier BV
publisher.none.fl_str_mv Elsevier
Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132184888475648

Registros relacionados