Diversity of Pneumocystis jirovecii Across Europe:
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1016/j.ebiom.2017.06.027 |
Resumo: | Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting > 500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11 − 20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥ 2 alleles for ≥ 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p < 0.001). The distribution of the alleles was significantly different (p < 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p < 0.001) and Gt16 with Belgium (5/5, p < 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients. |
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Diversity of Pneumocystis jirovecii Across Europe:A Multicentre Observational StudyEuropeGenotypingMicrosatellitesMixed infectionMLS typingPneumocystis jiroveciiTransmissionBiochemistry, Genetics and Molecular Biology(all)Infectious DiseasesEpidemiologySDG 3 - Good Health and Well-beingPneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting > 500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11 − 20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥ 2 alleles for ≥ 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p < 0.001). The distribution of the alleles was significantly different (p < 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p < 0.001) and Gt16 with Belgium (5/5, p < 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)TB, HIV and opportunistic diseases and pathogens (THOP)RUNAlanio, AlexandreGits-Muselli, MaudGuigue, NicolasDesnos-Ollivier, MarieCalderon, Enrique J.Di Cave, DavidDupont, DamienHamprecht, AxelHauser, Philippe M.Helweg-Larsen, JannikKicia, MartaLagrou, KatrienLengerova, MartinaMatos, OlgaMelchers, Willem J.G.Morio, FlorentNevez, GillesTotet, AnneWhite, Lewis P.Bretagne, Stéphane2018-05-15T22:06:14Z2017-08-012017-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttps://doi.org/10.1016/j.ebiom.2017.06.027eng2352-3964PURE: 3182179http://www.scopus.com/inward/record.url?scp=85021876565&partnerID=8YFLogxKhttps://doi.org/10.1016/j.ebiom.2017.06.027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:32:56Zoai:run.unl.pt:10362/37098Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:32:56Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study |
title |
Diversity of Pneumocystis jirovecii Across Europe: |
spellingShingle |
Diversity of Pneumocystis jirovecii Across Europe: Alanio, Alexandre Europe Genotyping Microsatellites Mixed infection MLS typing Pneumocystis jirovecii Transmission Biochemistry, Genetics and Molecular Biology(all) Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
title_short |
Diversity of Pneumocystis jirovecii Across Europe: |
title_full |
Diversity of Pneumocystis jirovecii Across Europe: |
title_fullStr |
Diversity of Pneumocystis jirovecii Across Europe: |
title_full_unstemmed |
Diversity of Pneumocystis jirovecii Across Europe: |
title_sort |
Diversity of Pneumocystis jirovecii Across Europe: |
author |
Alanio, Alexandre |
author_facet |
Alanio, Alexandre Gits-Muselli, Maud Guigue, Nicolas Desnos-Ollivier, Marie Calderon, Enrique J. Di Cave, David Dupont, Damien Hamprecht, Axel Hauser, Philippe M. Helweg-Larsen, Jannik Kicia, Marta Lagrou, Katrien Lengerova, Martina Matos, Olga Melchers, Willem J.G. Morio, Florent Nevez, Gilles Totet, Anne White, Lewis P. Bretagne, Stéphane |
author_role |
author |
author2 |
Gits-Muselli, Maud Guigue, Nicolas Desnos-Ollivier, Marie Calderon, Enrique J. Di Cave, David Dupont, Damien Hamprecht, Axel Hauser, Philippe M. Helweg-Larsen, Jannik Kicia, Marta Lagrou, Katrien Lengerova, Martina Matos, Olga Melchers, Willem J.G. Morio, Florent Nevez, Gilles Totet, Anne White, Lewis P. Bretagne, Stéphane |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) TB, HIV and opportunistic diseases and pathogens (THOP) RUN |
dc.contributor.author.fl_str_mv |
Alanio, Alexandre Gits-Muselli, Maud Guigue, Nicolas Desnos-Ollivier, Marie Calderon, Enrique J. Di Cave, David Dupont, Damien Hamprecht, Axel Hauser, Philippe M. Helweg-Larsen, Jannik Kicia, Marta Lagrou, Katrien Lengerova, Martina Matos, Olga Melchers, Willem J.G. Morio, Florent Nevez, Gilles Totet, Anne White, Lewis P. Bretagne, Stéphane |
dc.subject.por.fl_str_mv |
Europe Genotyping Microsatellites Mixed infection MLS typing Pneumocystis jirovecii Transmission Biochemistry, Genetics and Molecular Biology(all) Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
topic |
Europe Genotyping Microsatellites Mixed infection MLS typing Pneumocystis jirovecii Transmission Biochemistry, Genetics and Molecular Biology(all) Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
description |
Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting > 500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11 − 20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥ 2 alleles for ≥ 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p < 0.001). The distribution of the alleles was significantly different (p < 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p < 0.001) and Gt16 with Belgium (5/5, p < 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 2017-08-01T00:00:00Z 2018-05-15T22:06:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.ebiom.2017.06.027 |
url |
https://doi.org/10.1016/j.ebiom.2017.06.027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2352-3964 PURE: 3182179 http://www.scopus.com/inward/record.url?scp=85021876565&partnerID=8YFLogxK https://doi.org/10.1016/j.ebiom.2017.06.027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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9 application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817545640266170368 |