OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough

Detalhes bibliográficos
Autor(a) principal: Fiévet, Anouchka
Data de Publicação: 2021
Outros Autores: Merrouch, Meriem, Brasseur, Gaël, Eve, Danaé, Biondi, Emanuele G., Valette, Odile, Pauleta, Sofia R., Dolla, Alain, Dermoun, Zorah, Burlat, Bénédicte, Aubert, Corinne
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/128455
Resumo: SFCT‐ANR/BBB‐MET/0023/2012 IAP7/44, iPROS project) COST Action CA15133
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spelling OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris HildenboroughanaerobesDesulfovibrioenhancer binding proteiniron-sulfur proteinredox sensortranscriptional regulatorMicrobiologyMolecular BiologySFCT‐ANR/BBB‐MET/0023/2012 IAP7/44, iPROS project) COST Action CA15133Enhancer binding proteins (EBPs) are key players of σ54-regulation that control transcription in response to environmental signals. In the anaerobic microorganism Desulfovibrio vulgaris Hildenborough (DvH), orp operons have been previously shown to be coregulated by σ54-RNA polymerase, the integration host factor IHF and a cognate EBP, OrpR. In this study, ChIP-seq experiments indicated that the OrpR regulon consists of only the two divergent orp operons. In vivo data revealed that (i) OrpR is absolutely required for orp operons transcription, (ii) under anaerobic conditions, OrpR binds on the two dedicated DNA binding sites and leads to high expression levels of the orp operons, (iii) increasing the redox potential of the medium leads to a drastic down-regulation of the orp operons expression. Moreover, combining functional and biophysical studies on the anaerobically purified OrpR leads us to propose that OrpR senses redox potential variations via a redox-sensitive [4Fe–4S]2+ cluster in the sensory PAS domain. Overall, the study herein presents the first characterization of a new Fe–S redox regulator belonging to the σ54-dependent transcriptional regulator family probably advantageously selected by cells adapted to the anaerobic lifestyle to monitor redox stress conditions.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNFiévet, AnouchkaMerrouch, MeriemBrasseur, GaëlEve, DanaéBiondi, Emanuele G.Valette, OdilePauleta, Sofia R.Dolla, AlainDermoun, ZorahBurlat, BénédicteAubert, Corinne2021-11-29T23:39:30Z2021-072021-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/128455eng0950-382XPURE: 34405395https://doi.org/10.1111/mmi.14705info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:01Zoai:run.unl.pt:10362/128455Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:18.327941Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
title OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
spellingShingle OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
Fiévet, Anouchka
anaerobes
Desulfovibrio
enhancer binding protein
iron-sulfur protein
redox sensor
transcriptional regulator
Microbiology
Molecular Biology
title_short OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
title_full OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
title_fullStr OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
title_full_unstemmed OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
title_sort OrpR is a σ54-dependent activator using an iron-sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough
author Fiévet, Anouchka
author_facet Fiévet, Anouchka
Merrouch, Meriem
Brasseur, Gaël
Eve, Danaé
Biondi, Emanuele G.
Valette, Odile
Pauleta, Sofia R.
Dolla, Alain
Dermoun, Zorah
Burlat, Bénédicte
Aubert, Corinne
author_role author
author2 Merrouch, Meriem
Brasseur, Gaël
Eve, Danaé
Biondi, Emanuele G.
Valette, Odile
Pauleta, Sofia R.
Dolla, Alain
Dermoun, Zorah
Burlat, Bénédicte
Aubert, Corinne
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Fiévet, Anouchka
Merrouch, Meriem
Brasseur, Gaël
Eve, Danaé
Biondi, Emanuele G.
Valette, Odile
Pauleta, Sofia R.
Dolla, Alain
Dermoun, Zorah
Burlat, Bénédicte
Aubert, Corinne
dc.subject.por.fl_str_mv anaerobes
Desulfovibrio
enhancer binding protein
iron-sulfur protein
redox sensor
transcriptional regulator
Microbiology
Molecular Biology
topic anaerobes
Desulfovibrio
enhancer binding protein
iron-sulfur protein
redox sensor
transcriptional regulator
Microbiology
Molecular Biology
description SFCT‐ANR/BBB‐MET/0023/2012 IAP7/44, iPROS project) COST Action CA15133
publishDate 2021
dc.date.none.fl_str_mv 2021-11-29T23:39:30Z
2021-07
2021-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/128455
url http://hdl.handle.net/10362/128455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0950-382X
PURE: 34405395
https://doi.org/10.1111/mmi.14705
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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