Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Pedro M.
Data de Publicação: 2003
Outros Autores: Moura, Isabel, Macedo, Anjos L., Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/1662
Resumo: Inorganica Chimica Acta 356 (2003) 215-221
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spelling Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774Iron /sulfur cluster2 /[4Fe /4S] clustersFerredoxinMetalloproteinParamagnetic proteinNuclear magnetic resonanceDesulfovibrio desulfuricans ATCC 27774Inorganica Chimica Acta 356 (2003) 215-221A novel iron /sulfur containing protein, a ferredoxin (Fd), was purified to homogeneity from the extract of Desulfovibrio desulfuricans American type culture collection (ATCC) 27774. The purified protein is a 13.4 kDa homodimer with a polypeptide chain of 60 amino acids residues, containing eight cysteines that coordinate two [4Fe /4S] clusters. The protein is shown to be air sensitive and cluster conversions take place. We structurally characterize a redox state that contains two [4Fe /4S] cores. 1D and 2D 1H NMR studies are reported on form containing the clusters in the oxidized state. Based on the nuclear Overhauser effect (NOE), relaxation measurements and comparison of the present data with the available spectra of the analogous 8Fe Fds, the cluster ligands were specifically assigned to the eight-cysteinyl residuesElsevier B. V.RUNRodrigues, Pedro M.Moura, IsabelMacedo, Anjos L.Moura, José J. G.2008-09-25T09:41:51Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1662eng0020-1693info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:06:18Zoai:run.unl.pt:10362/1662Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:06:18Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
title Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
spellingShingle Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
Rodrigues, Pedro M.
Iron /sulfur cluster
2 /[4Fe /4S] clusters
Ferredoxin
Metalloprotein
Paramagnetic protein
Nuclear magnetic resonance
Desulfovibrio desulfuricans ATCC 27774
title_short Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
title_full Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
title_fullStr Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
title_full_unstemmed Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
title_sort Spectroscopic characterization of a novel 2 /[4Fe /4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774
author Rodrigues, Pedro M.
author_facet Rodrigues, Pedro M.
Moura, Isabel
Macedo, Anjos L.
Moura, José J. G.
author_role author
author2 Moura, Isabel
Macedo, Anjos L.
Moura, José J. G.
author2_role author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Rodrigues, Pedro M.
Moura, Isabel
Macedo, Anjos L.
Moura, José J. G.
dc.subject.por.fl_str_mv Iron /sulfur cluster
2 /[4Fe /4S] clusters
Ferredoxin
Metalloprotein
Paramagnetic protein
Nuclear magnetic resonance
Desulfovibrio desulfuricans ATCC 27774
topic Iron /sulfur cluster
2 /[4Fe /4S] clusters
Ferredoxin
Metalloprotein
Paramagnetic protein
Nuclear magnetic resonance
Desulfovibrio desulfuricans ATCC 27774
description Inorganica Chimica Acta 356 (2003) 215-221
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2008-09-25T09:41:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1662
url http://hdl.handle.net/10362/1662
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0020-1693
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B. V.
publisher.none.fl_str_mv Elsevier B. V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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