Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts

Detalhes bibliográficos
Autor(a) principal: Costa, Bruna C.
Data de Publicação: 2019
Outros Autores: Alves, Alexandra Manuela Vieira Cruz Pinto, Toptan, Fatih, Pinto, A. M. P., Grenho, Liliana, Fernandes, Maria H., Petrovykh, Dmitri Y., Rocha, L. A., Lisboa-Filho, Paulo N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/66292
Resumo: Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.
id RCAP_49ff9521122751f1e5ef022988f0455d
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/66292
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Exposure effects of endotoxin-free titanium-based wear particles to human osteoblastsTitanium implantsDegradationWear particlesEndotoxinsBiological effectsScience & TechnologyTitanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP (2015/50280-5 and 2017/24300-4), Fundacao para a Ciencia e Tecnologia - FCT (UID/EEA/04436/2013), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES (Finance Code 0001), FCT/CAPES Joint Research Project (99999.008666/2014-08), FCT COMPETE 2020 (POCI-01-0145-FEDER-006941 and POCI-01-0145-FEDER-007265) and M-ERA-NET (0001/2015).ElsevierUniversidade do MinhoCosta, Bruna C.Alves, Alexandra Manuela Vieira Cruz PintoToptan, FatihPinto, A. M. P.Grenho, LilianaFernandes, Maria H.Petrovykh, Dmitri Y.Rocha, L. A.Lisboa-Filho, Paulo N.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66292eng1751-61611878-018010.1016/j.jmbbm.2019.04.00330999211info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:36Zoai:repositorium.sdum.uminho.pt:1822/66292Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:14.217629Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
spellingShingle Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
Costa, Bruna C.
Titanium implants
Degradation
Wear particles
Endotoxins
Biological effects
Science & Technology
title_short Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_full Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_fullStr Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_full_unstemmed Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_sort Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
author Costa, Bruna C.
author_facet Costa, Bruna C.
Alves, Alexandra Manuela Vieira Cruz Pinto
Toptan, Fatih
Pinto, A. M. P.
Grenho, Liliana
Fernandes, Maria H.
Petrovykh, Dmitri Y.
Rocha, L. A.
Lisboa-Filho, Paulo N.
author_role author
author2 Alves, Alexandra Manuela Vieira Cruz Pinto
Toptan, Fatih
Pinto, A. M. P.
Grenho, Liliana
Fernandes, Maria H.
Petrovykh, Dmitri Y.
Rocha, L. A.
Lisboa-Filho, Paulo N.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Costa, Bruna C.
Alves, Alexandra Manuela Vieira Cruz Pinto
Toptan, Fatih
Pinto, A. M. P.
Grenho, Liliana
Fernandes, Maria H.
Petrovykh, Dmitri Y.
Rocha, L. A.
Lisboa-Filho, Paulo N.
dc.subject.por.fl_str_mv Titanium implants
Degradation
Wear particles
Endotoxins
Biological effects
Science & Technology
topic Titanium implants
Degradation
Wear particles
Endotoxins
Biological effects
Science & Technology
description Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/66292
url http://hdl.handle.net/1822/66292
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1751-6161
1878-0180
10.1016/j.jmbbm.2019.04.003
30999211
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132890774110208