Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts

Detalhes bibliográficos
Autor(a) principal: Costa, Bruna C. [UNESP]
Data de Publicação: 2019
Outros Autores: Alves, Alexandra C., Toptan, Fatih, Pinto, Ana M., Grenho, Liliana, Fernandes, Maria H., Petrovykh, Dmitri Y., Rocha, Luís A. [UNESP], Lisboa-Filho, Paulo N. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jmbbm.2019.04.003
http://hdl.handle.net/11449/187549
Resumo: Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic-based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.
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spelling Exposure effects of endotoxin-free titanium-based wear particles to human osteoblastsBiological effectsDegradationEndotoxinsTitanium implantsWear particlesTitanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic-based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program in Materials Science and Technology POSMAT UNESP São Paulo State UniversityCMEMS-UMinho Center for Micro Electro Mechanical Systems University of Minho, AzurémDepartment of Mechanical Engineering University of Minho, AzurémLaboratory for Bone Metabolism and Regeneration Faculty of Dental Medicine U. Porto FMDUPLAQV/REQUIMTE U. PortoInternational Iberian Nanotechnology LaboratoryDepartment of Physics UNESP São Paulo State UniversityGraduate Program in Materials Science and Technology POSMAT UNESP São Paulo State UniversityDepartment of Physics UNESP São Paulo State UniversityCAPES: 0001Universidade Estadual Paulista (Unesp)University of MinhoFMDUPU. PortoInternational Iberian Nanotechnology LaboratoryCosta, Bruna C. [UNESP]Alves, Alexandra C.Toptan, FatihPinto, Ana M.Grenho, LilianaFernandes, Maria H.Petrovykh, Dmitri Y.Rocha, Luís A. [UNESP]Lisboa-Filho, Paulo N. [UNESP]2019-10-06T15:39:45Z2019-10-06T15:39:45Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article143-152http://dx.doi.org/10.1016/j.jmbbm.2019.04.003Journal of the Mechanical Behavior of Biomedical Materials, v. 95, p. 143-152.1878-01801751-6161http://hdl.handle.net/11449/18754910.1016/j.jmbbm.2019.04.0032-s2.0-8506419816013538624145320050000-0002-7734-4069Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Mechanical Behavior of Biomedical Materialsinfo:eu-repo/semantics/openAccess2021-10-23T00:57:21Zoai:repositorio.unesp.br:11449/187549Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:47:10.780763Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
spellingShingle Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
Costa, Bruna C. [UNESP]
Biological effects
Degradation
Endotoxins
Titanium implants
Wear particles
title_short Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_full Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_fullStr Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_full_unstemmed Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
title_sort Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
author Costa, Bruna C. [UNESP]
author_facet Costa, Bruna C. [UNESP]
Alves, Alexandra C.
Toptan, Fatih
Pinto, Ana M.
Grenho, Liliana
Fernandes, Maria H.
Petrovykh, Dmitri Y.
Rocha, Luís A. [UNESP]
Lisboa-Filho, Paulo N. [UNESP]
author_role author
author2 Alves, Alexandra C.
Toptan, Fatih
Pinto, Ana M.
Grenho, Liliana
Fernandes, Maria H.
Petrovykh, Dmitri Y.
Rocha, Luís A. [UNESP]
Lisboa-Filho, Paulo N. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Minho
FMDUP
U. Porto
International Iberian Nanotechnology Laboratory
dc.contributor.author.fl_str_mv Costa, Bruna C. [UNESP]
Alves, Alexandra C.
Toptan, Fatih
Pinto, Ana M.
Grenho, Liliana
Fernandes, Maria H.
Petrovykh, Dmitri Y.
Rocha, Luís A. [UNESP]
Lisboa-Filho, Paulo N. [UNESP]
dc.subject.por.fl_str_mv Biological effects
Degradation
Endotoxins
Titanium implants
Wear particles
topic Biological effects
Degradation
Endotoxins
Titanium implants
Wear particles
description Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic-based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:39:45Z
2019-10-06T15:39:45Z
2019-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jmbbm.2019.04.003
Journal of the Mechanical Behavior of Biomedical Materials, v. 95, p. 143-152.
1878-0180
1751-6161
http://hdl.handle.net/11449/187549
10.1016/j.jmbbm.2019.04.003
2-s2.0-85064198160
1353862414532005
0000-0002-7734-4069
url http://dx.doi.org/10.1016/j.jmbbm.2019.04.003
http://hdl.handle.net/11449/187549
identifier_str_mv Journal of the Mechanical Behavior of Biomedical Materials, v. 95, p. 143-152.
1878-0180
1751-6161
10.1016/j.jmbbm.2019.04.003
2-s2.0-85064198160
1353862414532005
0000-0002-7734-4069
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Mechanical Behavior of Biomedical Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 143-152
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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