Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jmbbm.2019.04.003 http://hdl.handle.net/11449/187549 |
Resumo: | Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic-based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated. |
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Exposure effects of endotoxin-free titanium-based wear particles to human osteoblastsBiological effectsDegradationEndotoxinsTitanium implantsWear particlesTitanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic-based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program in Materials Science and Technology POSMAT UNESP São Paulo State UniversityCMEMS-UMinho Center for Micro Electro Mechanical Systems University of Minho, AzurémDepartment of Mechanical Engineering University of Minho, AzurémLaboratory for Bone Metabolism and Regeneration Faculty of Dental Medicine U. Porto FMDUPLAQV/REQUIMTE U. PortoInternational Iberian Nanotechnology LaboratoryDepartment of Physics UNESP São Paulo State UniversityGraduate Program in Materials Science and Technology POSMAT UNESP São Paulo State UniversityDepartment of Physics UNESP São Paulo State UniversityCAPES: 0001Universidade Estadual Paulista (Unesp)University of MinhoFMDUPU. PortoInternational Iberian Nanotechnology LaboratoryCosta, Bruna C. [UNESP]Alves, Alexandra C.Toptan, FatihPinto, Ana M.Grenho, LilianaFernandes, Maria H.Petrovykh, Dmitri Y.Rocha, Luís A. [UNESP]Lisboa-Filho, Paulo N. [UNESP]2019-10-06T15:39:45Z2019-10-06T15:39:45Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article143-152http://dx.doi.org/10.1016/j.jmbbm.2019.04.003Journal of the Mechanical Behavior of Biomedical Materials, v. 95, p. 143-152.1878-01801751-6161http://hdl.handle.net/11449/18754910.1016/j.jmbbm.2019.04.0032-s2.0-8506419816013538624145320050000-0002-7734-4069Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Mechanical Behavior of Biomedical Materialsinfo:eu-repo/semantics/openAccess2021-10-23T00:57:21Zoai:repositorio.unesp.br:11449/187549Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:47:10.780763Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
title |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
spellingShingle |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts Costa, Bruna C. [UNESP] Biological effects Degradation Endotoxins Titanium implants Wear particles |
title_short |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
title_full |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
title_fullStr |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
title_full_unstemmed |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
title_sort |
Exposure effects of endotoxin-free titanium-based wear particles to human osteoblasts |
author |
Costa, Bruna C. [UNESP] |
author_facet |
Costa, Bruna C. [UNESP] Alves, Alexandra C. Toptan, Fatih Pinto, Ana M. Grenho, Liliana Fernandes, Maria H. Petrovykh, Dmitri Y. Rocha, Luís A. [UNESP] Lisboa-Filho, Paulo N. [UNESP] |
author_role |
author |
author2 |
Alves, Alexandra C. Toptan, Fatih Pinto, Ana M. Grenho, Liliana Fernandes, Maria H. Petrovykh, Dmitri Y. Rocha, Luís A. [UNESP] Lisboa-Filho, Paulo N. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Minho FMDUP U. Porto International Iberian Nanotechnology Laboratory |
dc.contributor.author.fl_str_mv |
Costa, Bruna C. [UNESP] Alves, Alexandra C. Toptan, Fatih Pinto, Ana M. Grenho, Liliana Fernandes, Maria H. Petrovykh, Dmitri Y. Rocha, Luís A. [UNESP] Lisboa-Filho, Paulo N. [UNESP] |
dc.subject.por.fl_str_mv |
Biological effects Degradation Endotoxins Titanium implants Wear particles |
topic |
Biological effects Degradation Endotoxins Titanium implants Wear particles |
description |
Titanium-based materials are widely employed by the biomedical industry in orthopedic and dental implants. However, when placed into the human body, these materials are highly susceptible to degradation processes, such as corrosion, wear, and tribocorrosion. As a consequence, metallic ions or particles (debris) may be released, and although several studies have been conducted in recent years to better understand the effects of their exposure to living cells, a consensual opinion has not yet been obtained. In this work, we produced metallic-based wear particles by tribological tests carried out on Ti-6Al-4V and Ti-15Zr-15Mo alloys. They were posteriorly physicochemically characterized according to their crystal structure, size, morphology, and chemical composition and compared to Ti-6Al-4V commercially available particles. Finally, adsorbed endotoxins were removed (by applying a specific thermal treatment) and endotoxin-free particles were used in cell experiments to evaluate effects of their exposure to human osteoblasts (MG-63 and HOb), namely cell viability/metabolism, proinflammatory cytokine production (IL-6 and PGE2), and susceptibility to internalization processes. Our results indicate that tribologically-obtained wear particles exhibit fundamental differences in terms of size (smaller) and morphology (irregular shapes and rough surfaces) when compared to the commercial ones. Consequently, both Ti-6Al-4V and Ti-15Zr-15Mo particles were able to induce more pronounced effects on cell viability (decrease) and cytokine production (increase) than did Ti-6Al-4V commercial particles. Furthermore, both types of wear particles penetrated osteoblast membranes and were internalized by the cells. Influences on cytokine production by endotoxins were also demonstrated. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:39:45Z 2019-10-06T15:39:45Z 2019-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jmbbm.2019.04.003 Journal of the Mechanical Behavior of Biomedical Materials, v. 95, p. 143-152. 1878-0180 1751-6161 http://hdl.handle.net/11449/187549 10.1016/j.jmbbm.2019.04.003 2-s2.0-85064198160 1353862414532005 0000-0002-7734-4069 |
url |
http://dx.doi.org/10.1016/j.jmbbm.2019.04.003 http://hdl.handle.net/11449/187549 |
identifier_str_mv |
Journal of the Mechanical Behavior of Biomedical Materials, v. 95, p. 143-152. 1878-0180 1751-6161 10.1016/j.jmbbm.2019.04.003 2-s2.0-85064198160 1353862414532005 0000-0002-7734-4069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the Mechanical Behavior of Biomedical Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
143-152 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128562899714048 |