Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton

Detalhes bibliográficos
Autor(a) principal: Lhocine, Nouara
Data de Publicação: 2015
Outros Autores: Arena, Ellen T., Bomme, Perrine, Ubelmann, Florent, Prévost, Marie Christine, Robine, Sylvie, Sansonetti, Philippe J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/149784
Resumo: Funding Information: We thank R. Friedman, C. Mulet and T. Pedron for technical help. We thank T. Marlovits for antibodies, H.D. Hardt and J. Galan for Salmonella strains, and D. Zhou and V. Koronakis for plasmids. We acknowledge France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INSB-04-01, «Investments for the future»). This work was supported by the ERC (P.S. Advanced Grant HOMEOEPITH, number 232798). P.J.S. is an HHMI senior foreign scholar. The authors declare no conflict of interest. Publisher Copyright: © 2015 Elsevier Inc.
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spelling Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeletonParasitologyMicrobiologyVirologyFunding Information: We thank R. Friedman, C. Mulet and T. Pedron for technical help. We thank T. Marlovits for antibodies, H.D. Hardt and J. Galan for Salmonella strains, and D. Zhou and V. Koronakis for plasmids. We acknowledge France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INSB-04-01, «Investments for the future»). This work was supported by the ERC (P.S. Advanced Grant HOMEOEPITH, number 232798). P.J.S. is an HHMI senior foreign scholar. The authors declare no conflict of interest. Publisher Copyright: © 2015 Elsevier Inc.Salmonella invasion of intestinal epithelial cells requires extensive, though transient, actin modifications at the site of bacterial entry. The actin-modifying protein villin is present in the brush border where it participates in the constitution of microvilli and in epithelial restitution after damage through its actin-severing activity. We investigated a possible role for villin in Salmonella invasion. The absence of villin, which is normally located at the bacterial entry site, leads to a decrease in Salmonella invasion. Villin is necessary for early membrane-associated processes and for optimal ruffle assembly by balancing the steady-state level of actin. The severing activity of villin is important for Salmonella invasion in vivo. The bacterial phosphatase SptP tightly regulates villin phosphorylation, while the actin-binding effector SipA protects F-actin and counterbalances villin-severing activity. Thus, villin plays an important role in establishing the balance between actin polymerization and actin severing to facilitate the initial steps of Salmonella entry.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNLhocine, NouaraArena, Ellen T.Bomme, PerrineUbelmann, FlorentPrévost, Marie ChristineRobine, SylvieSansonetti, Philippe J.2023-02-27T22:21:26Z2015-02-112015-02-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/149784eng1931-3128PURE: 47545843https://doi.org/10.1016/j.chom.2014.12.003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:31:37Zoai:run.unl.pt:10362/149784Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:51.177931Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
title Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
spellingShingle Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
Lhocine, Nouara
Parasitology
Microbiology
Virology
title_short Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
title_full Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
title_fullStr Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
title_full_unstemmed Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
title_sort Apical invasion of intestinal epithelial cells by salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton
author Lhocine, Nouara
author_facet Lhocine, Nouara
Arena, Ellen T.
Bomme, Perrine
Ubelmann, Florent
Prévost, Marie Christine
Robine, Sylvie
Sansonetti, Philippe J.
author_role author
author2 Arena, Ellen T.
Bomme, Perrine
Ubelmann, Florent
Prévost, Marie Christine
Robine, Sylvie
Sansonetti, Philippe J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Lhocine, Nouara
Arena, Ellen T.
Bomme, Perrine
Ubelmann, Florent
Prévost, Marie Christine
Robine, Sylvie
Sansonetti, Philippe J.
dc.subject.por.fl_str_mv Parasitology
Microbiology
Virology
topic Parasitology
Microbiology
Virology
description Funding Information: We thank R. Friedman, C. Mulet and T. Pedron for technical help. We thank T. Marlovits for antibodies, H.D. Hardt and J. Galan for Salmonella strains, and D. Zhou and V. Koronakis for plasmids. We acknowledge France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INSB-04-01, «Investments for the future»). This work was supported by the ERC (P.S. Advanced Grant HOMEOEPITH, number 232798). P.J.S. is an HHMI senior foreign scholar. The authors declare no conflict of interest. Publisher Copyright: © 2015 Elsevier Inc.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-11
2015-02-11T00:00:00Z
2023-02-27T22:21:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/149784
url http://hdl.handle.net/10362/149784
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1931-3128
PURE: 47545843
https://doi.org/10.1016/j.chom.2014.12.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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