First isolates of Leptospira spp., from rodents captured in Angola

Detalhes bibliográficos
Autor(a) principal: Fortes-Gabriel, Elsa
Data de Publicação: 2016
Outros Autores: Carreira, Teresa, Vieira, Maria Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.4269/ajtmh.15-0027
Resumo: Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola.
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spelling First isolates of Leptospira spp., from rodents captured in AngolaParasitologyInfectious DiseasesVirologySDG 3 - Good Health and Well-beingRodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola.Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)RUNFortes-Gabriel, ElsaCarreira, TeresaVieira, Maria Luísa2018-05-16T22:04:52Z2016-05-012016-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttps://doi.org/10.4269/ajtmh.15-0027eng0002-9637PURE: 2450693http://www.scopus.com/inward/record.url?scp=84966661204&partnerID=8YFLogxKhttps://doi.org/10.4269/ajtmh.15-0027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:45Zoai:run.unl.pt:10362/37188Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:50.113464Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv First isolates of Leptospira spp., from rodents captured in Angola
title First isolates of Leptospira spp., from rodents captured in Angola
spellingShingle First isolates of Leptospira spp., from rodents captured in Angola
Fortes-Gabriel, Elsa
Parasitology
Infectious Diseases
Virology
SDG 3 - Good Health and Well-being
title_short First isolates of Leptospira spp., from rodents captured in Angola
title_full First isolates of Leptospira spp., from rodents captured in Angola
title_fullStr First isolates of Leptospira spp., from rodents captured in Angola
title_full_unstemmed First isolates of Leptospira spp., from rodents captured in Angola
title_sort First isolates of Leptospira spp., from rodents captured in Angola
author Fortes-Gabriel, Elsa
author_facet Fortes-Gabriel, Elsa
Carreira, Teresa
Vieira, Maria Luísa
author_role author
author2 Carreira, Teresa
Vieira, Maria Luísa
author2_role author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
Global Health and Tropical Medicine (GHTM)
RUN
dc.contributor.author.fl_str_mv Fortes-Gabriel, Elsa
Carreira, Teresa
Vieira, Maria Luísa
dc.subject.por.fl_str_mv Parasitology
Infectious Diseases
Virology
SDG 3 - Good Health and Well-being
topic Parasitology
Infectious Diseases
Virology
SDG 3 - Good Health and Well-being
description Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
2016-05-01T00:00:00Z
2018-05-16T22:04:52Z
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dc.language.iso.fl_str_mv eng
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PURE: 2450693
http://www.scopus.com/inward/record.url?scp=84966661204&partnerID=8YFLogxK
https://doi.org/10.4269/ajtmh.15-0027
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