BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH

Detalhes bibliográficos
Autor(a) principal: Quintana-Hayashi, Macarena P.
Data de Publicação: 2018
Outros Autores: Rocha, Raquel, Padra, Médea, Thorell, Anders, Jin, Chunsheng, Karlsson, Niclas G., Roxo-Rosa, Mónica, Oleastro, Mónica, Lindén, Sara K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/149770
Resumo: Funding This work was supported by the Familjen Erling-Perssons Stiftelse;Fundação para a Ciência e a Tecnologia [FCTPTDC/BIM-MEC/1051/2012];Swedish research council [521-2011-2370];Swedish research council [621-2014-4361]; Swedish cancer society;Åke Wibergs ;Ragnar Söderbergs stiftelse;Stiftelserna Wilhelm och Martina Lundgrens;Svenska Forskningsrådet Formas [221-2013-590];Svenska Forskningsrådet Formas [221-2011-1036].
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spelling BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pHadhesinbabAbindingchildrenglycosylationHelicobacter pyloriMucinpeptic ulcerpHvirulenceParasitologyMicrobiologyImmunologyMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding This work was supported by the Familjen Erling-Perssons Stiftelse;Fundação para a Ciência e a Tecnologia [FCTPTDC/BIM-MEC/1051/2012];Swedish research council [521-2011-2370];Swedish research council [621-2014-4361]; Swedish cancer society;Åke Wibergs ;Ragnar Söderbergs stiftelse;Stiftelserna Wilhelm och Martina Lundgrens;Svenska Forskningsrådet Formas [221-2013-590];Svenska Forskningsrådet Formas [221-2011-1036].Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins. Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Leb), Sialyl-Lewis x (SLex) or LacdiNAc. In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Leb was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Leb at acidic and neutral pH, and to SLex and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNQuintana-Hayashi, Macarena P.Rocha, RaquelPadra, MédeaThorell, AndersJin, ChunshengKarlsson, Niclas G.Roxo-Rosa, MónicaOleastro, MónicaLindén, Sara K.2023-02-27T22:17:04Z2018-012018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/149770eng2150-5608PURE: 6403998https://doi.org/10.1080/21505594.2018.1532243info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:31:36Zoai:run.unl.pt:10362/149770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:50.936985Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
spellingShingle BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
Quintana-Hayashi, Macarena P.
adhesin
babA
binding
children
glycosylation
Helicobacter pylori
Mucin
peptic ulcer
pH
virulence
Parasitology
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_full BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_fullStr BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_full_unstemmed BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_sort BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
author Quintana-Hayashi, Macarena P.
author_facet Quintana-Hayashi, Macarena P.
Rocha, Raquel
Padra, Médea
Thorell, Anders
Jin, Chunsheng
Karlsson, Niclas G.
Roxo-Rosa, Mónica
Oleastro, Mónica
Lindén, Sara K.
author_role author
author2 Rocha, Raquel
Padra, Médea
Thorell, Anders
Jin, Chunsheng
Karlsson, Niclas G.
Roxo-Rosa, Mónica
Oleastro, Mónica
Lindén, Sara K.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Quintana-Hayashi, Macarena P.
Rocha, Raquel
Padra, Médea
Thorell, Anders
Jin, Chunsheng
Karlsson, Niclas G.
Roxo-Rosa, Mónica
Oleastro, Mónica
Lindén, Sara K.
dc.subject.por.fl_str_mv adhesin
babA
binding
children
glycosylation
Helicobacter pylori
Mucin
peptic ulcer
pH
virulence
Parasitology
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic adhesin
babA
binding
children
glycosylation
Helicobacter pylori
Mucin
peptic ulcer
pH
virulence
Parasitology
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding This work was supported by the Familjen Erling-Perssons Stiftelse;Fundação para a Ciência e a Tecnologia [FCTPTDC/BIM-MEC/1051/2012];Swedish research council [521-2011-2370];Swedish research council [621-2014-4361]; Swedish cancer society;Åke Wibergs ;Ragnar Söderbergs stiftelse;Stiftelserna Wilhelm och Martina Lundgrens;Svenska Forskningsrådet Formas [221-2013-590];Svenska Forskningsrådet Formas [221-2011-1036].
publishDate 2018
dc.date.none.fl_str_mv 2018-01
2018-01-01T00:00:00Z
2023-02-27T22:17:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/149770
url http://hdl.handle.net/10362/149770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2150-5608
PURE: 6403998
https://doi.org/10.1080/21505594.2018.1532243
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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