Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration

Detalhes bibliográficos
Autor(a) principal: Casalou, Cristina
Data de Publicação: 2014
Outros Autores: Seixas,  Cecília, Portelinha, Ana, Pintado, Petra, Barros, Mafalda, Ramalho, José da Silva, Lopes, Susana Santos, Barral, Duarte
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/21937
Resumo: This work was supported by a Marie Curie International Reintegration Grant [grant number Marie Curie_PIRG05-GA-2009-247726 to D.C.B.]; by postdoctoral fellowships from Fundacao para a Ciencia e Tecnologia (FCT) [grant numbers SFRH/BPD/78561/2011 to C.C., SFRH/BPD/32323/2006 to C.S.]; a PhD fellowship from FCT [grant number SFRH/BD/68502/2010 to A.P.]; and a FCT grant [grant number PTDC/SAU-OBD/103981/2008 to S.L.].
id RCAP_4b8f7b9729f41b470c9e0dd2abf972ba
oai_identifier_str oai:run.unl.pt:10362/21937
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migrationActin cytoskeletonLOCALIZATIONArl13bPROTEINSWound healingMACROPINOCYTOSISCircular dorsal ruffleSmall G proteinZEBRAFISHPlatelet-derived growth factor signalingPRIMARY CILIAMyosin heavy chain IIAACTINMyh9LEADJOUBERT-SYNDROMERECEPTORROLESArl13bMyosin heavy chain IIAMyh9Circular dorsal ruffleSmall G proteinActin cytoskeletonPlatelet-derived growth factor signalingWound healingThis work was supported by a Marie Curie International Reintegration Grant [grant number Marie Curie_PIRG05-GA-2009-247726 to D.C.B.]; by postdoctoral fellowships from Fundacao para a Ciencia e Tecnologia (FCT) [grant numbers SFRH/BPD/78561/2011 to C.C., SFRH/BPD/32323/2006 to C.S.]; a PhD fellowship from FCT [grant number SFRH/BD/68502/2010 to A.P.]; and a FCT grant [grant number PTDC/SAU-OBD/103981/2008 to S.L.].The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic and interacts with actin. Herein, we report that the non-muscle myosin heavy chain IIA, also known as Myh9, is an Arl13b effector. Moreover, we found that both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation and are required for their formation. CDRs are ring-shaped actin-dependent structures formed on the dorsal cell surface and are involved in diverse processes, such as macropinocytosis, integrin recycling, internalization of receptor tyrosine kinases and cell migration. We found that Arl13b or Myh9 silencing impaired cell migration, suggesting that Arl13b is required for this function through the interaction with Myh9. Moreover, Arl13b silencing impaired neural crest cell migration in zebrafish embryos. Furthermore, we showed that Arl13b is required for the formation of CDRs in migrating cells. Thus, our results indicate a new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9, by driving the formation of CDRs necessary for cell migration.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCasalou, CristinaSeixas,  CecíliaPortelinha, AnaPintado, PetraBarros, MafaldaRamalho, José da SilvaLopes, Susana SantosBarral, Duarte2017-07-13T22:00:47Z2014-06-152014-06-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13application/pdfhttp://hdl.handle.net/10362/21937eng0021-9533PURE: 390083https://doi.org/10.1242/jcs.143446info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:09:05Zoai:run.unl.pt:10362/21937Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:03.005637Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
title Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
spellingShingle Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
Casalou, Cristina
Actin cytoskeleton
LOCALIZATION
Arl13b
PROTEINS
Wound healing
MACROPINOCYTOSIS
Circular dorsal ruffle
Small G protein
ZEBRAFISH
Platelet-derived growth factor signaling
PRIMARY CILIA
Myosin heavy chain IIA
ACTIN
Myh9
LEAD
JOUBERT-SYNDROME
RECEPTOR
ROLES
Arl13b
Myosin heavy chain IIA
Myh9
Circular dorsal ruffle
Small G protein
Actin cytoskeleton
Platelet-derived growth factor signaling
Wound healing
title_short Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
title_full Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
title_fullStr Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
title_full_unstemmed Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
title_sort Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration
author Casalou, Cristina
author_facet Casalou, Cristina
Seixas,  Cecília
Portelinha, Ana
Pintado, Petra
Barros, Mafalda
Ramalho, José da Silva
Lopes, Susana Santos
Barral, Duarte
author_role author
author2 Seixas,  Cecília
Portelinha, Ana
Pintado, Petra
Barros, Mafalda
Ramalho, José da Silva
Lopes, Susana Santos
Barral, Duarte
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Casalou, Cristina
Seixas,  Cecília
Portelinha, Ana
Pintado, Petra
Barros, Mafalda
Ramalho, José da Silva
Lopes, Susana Santos
Barral, Duarte
dc.subject.por.fl_str_mv Actin cytoskeleton
LOCALIZATION
Arl13b
PROTEINS
Wound healing
MACROPINOCYTOSIS
Circular dorsal ruffle
Small G protein
ZEBRAFISH
Platelet-derived growth factor signaling
PRIMARY CILIA
Myosin heavy chain IIA
ACTIN
Myh9
LEAD
JOUBERT-SYNDROME
RECEPTOR
ROLES
Arl13b
Myosin heavy chain IIA
Myh9
Circular dorsal ruffle
Small G protein
Actin cytoskeleton
Platelet-derived growth factor signaling
Wound healing
topic Actin cytoskeleton
LOCALIZATION
Arl13b
PROTEINS
Wound healing
MACROPINOCYTOSIS
Circular dorsal ruffle
Small G protein
ZEBRAFISH
Platelet-derived growth factor signaling
PRIMARY CILIA
Myosin heavy chain IIA
ACTIN
Myh9
LEAD
JOUBERT-SYNDROME
RECEPTOR
ROLES
Arl13b
Myosin heavy chain IIA
Myh9
Circular dorsal ruffle
Small G protein
Actin cytoskeleton
Platelet-derived growth factor signaling
Wound healing
description This work was supported by a Marie Curie International Reintegration Grant [grant number Marie Curie_PIRG05-GA-2009-247726 to D.C.B.]; by postdoctoral fellowships from Fundacao para a Ciencia e Tecnologia (FCT) [grant numbers SFRH/BPD/78561/2011 to C.C., SFRH/BPD/32323/2006 to C.S.]; a PhD fellowship from FCT [grant number SFRH/BD/68502/2010 to A.P.]; and a FCT grant [grant number PTDC/SAU-OBD/103981/2008 to S.L.].
publishDate 2014
dc.date.none.fl_str_mv 2014-06-15
2014-06-15T00:00:00Z
2017-07-13T22:00:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/21937
url http://hdl.handle.net/10362/21937
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9533
PURE: 390083
https://doi.org/10.1242/jcs.143446
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137899837390848

Registros relacionados