Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses

Detalhes bibliográficos
Autor(a) principal: Monteiro, Hugo R.
Data de Publicação: 2019
Outros Autores: Pestana, João L. T., Novais, Sara C., Leston, Sara, Ramos, Fernando, Soares, Amadeu M. V. M., Devreese, Bart, Lemos, Marco F. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/4213
Resumo: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UID/MAR/04292/2019; CESAM: UID/ AMB/50017/2019, FCT/MCTES through national funds, the cofunding by the FEDER within the PT2020 Partnership Agreement and Compete 2020; Project PROTEOME (PTDC/AAG-MAA/1302/2014) cofinanced by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) co-funded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro, Sara C. Novais, and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, SFRH/BPD/94500/2013, IF/01420/2015, respectively).
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spelling Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responsesAquatic insectsBiomarkersEnvironmental proteomicsPhenylpyrazole insecticidesSub-lethal toxicityThis study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UID/MAR/04292/2019; CESAM: UID/ AMB/50017/2019, FCT/MCTES through national funds, the cofunding by the FEDER within the PT2020 Partnership Agreement and Compete 2020; Project PROTEOME (PTDC/AAG-MAA/1302/2014) cofinanced by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) co-funded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro, Sara C. Novais, and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, SFRH/BPD/94500/2013, IF/01420/2015, respectively).Fipronil is a phenylpyrazole insecticide that entered the market to replace organochlorides and organophosphates. Fipronil impairs the regular inhibition of nerve impulses that ultimately result in paralysis and death of insects. Because of its use as a pest control, and due to runoff events, fipronil has been detected in freshwater systems near agricultural areas, and therefore might represent a threat to non-target aquatic organisms. In this study, the toxicity of fipronil to the freshwater midge Chironomus riparius was investigated at biochemical, molecular, and whole organism (e.g. growth, emergence, and behavior) levels. At the individual level, chronic (28 days) exposure to fipronil resulted in reduced larval growth and emergence with a lowest observed effect concentration (LOEC) of 0.081 μg L−1. Adult weight, which is directly linked to the flying performance and fecundity of midges, was also affected (LOEC = 0.040 μg L−1). Additionally, behavioral changes such as irregular burrowing behavior of C. riparius larvae (EC50 = 0.084 μg L−1) and impairment of adult flying performance were observed. At a biochemical level, acute (48 h) exposure to fipronil increased cellular oxygen consumption (as indicated by the increase of electron transport system (ETS) activity) and decreased antioxidant and detoxification defenses (as suggested by the decrease in catalase (CAT) and glutathione S-transferase (GST) activities). Exposure to fipronil also caused alterations in the fatty acid profile of C. riparius, since high levels of stearidonic acid (SDA) were observed. A comparison between exposed and nonexposed larvae also revealed alterations in the expression of globins, cytoskeleton and motor proteins, and proteins involved in protein biosynthesis. These alterations may aid in the interpretation of potential mechanisms of action that lead to the effects observed at the organism level. Present results show that environmentally relevant concentrations of fipronil are toxic to chironomid populations which call for monitoring of phenylpyrazole insecticides and of their ecological effects in freshwaters. Present results also emphasize the importance of complementing ecotoxicological data with molecular approaches such as proteomics, for a better interpretation of the mode of action of insecticides in aquatic invertebrates.IC-OnlineMonteiro, Hugo R.Pestana, João L. T.Novais, Sara C.Leston, SaraRamos, FernandoSoares, Amadeu M. V. M.Devreese, BartLemos, Marco F. L.2019-10-16T10:53:53Z2019-112019-10-07T10:35:53Z2019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4213engMonteiro, H. R., Pestana, J. L. T., Soares, A. M. V. M., Novais, S. C., Lemos, M. F. L., Devreese, B., … Ramos, F. (2019). Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses. Aquatic Toxicology, 216. https://doi.org/10.1016/j.aquatox.2019.1052920166-445Xcv-prod-76097910.1016/j.aquatox.2019.105292metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:48:47Zoai:iconline.ipleiria.pt:10400.8/4213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:07.229001Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
title Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
spellingShingle Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
Monteiro, Hugo R.
Aquatic insects
Biomarkers
Environmental proteomics
Phenylpyrazole insecticides
Sub-lethal toxicity
title_short Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
title_full Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
title_fullStr Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
title_full_unstemmed Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
title_sort Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses
author Monteiro, Hugo R.
author_facet Monteiro, Hugo R.
Pestana, João L. T.
Novais, Sara C.
Leston, Sara
Ramos, Fernando
Soares, Amadeu M. V. M.
Devreese, Bart
Lemos, Marco F. L.
author_role author
author2 Pestana, João L. T.
Novais, Sara C.
Leston, Sara
Ramos, Fernando
Soares, Amadeu M. V. M.
Devreese, Bart
Lemos, Marco F. L.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Monteiro, Hugo R.
Pestana, João L. T.
Novais, Sara C.
Leston, Sara
Ramos, Fernando
Soares, Amadeu M. V. M.
Devreese, Bart
Lemos, Marco F. L.
dc.subject.por.fl_str_mv Aquatic insects
Biomarkers
Environmental proteomics
Phenylpyrazole insecticides
Sub-lethal toxicity
topic Aquatic insects
Biomarkers
Environmental proteomics
Phenylpyrazole insecticides
Sub-lethal toxicity
description This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UID/MAR/04292/2019; CESAM: UID/ AMB/50017/2019, FCT/MCTES through national funds, the cofunding by the FEDER within the PT2020 Partnership Agreement and Compete 2020; Project PROTEOME (PTDC/AAG-MAA/1302/2014) cofinanced by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) co-funded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro, Sara C. Novais, and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, SFRH/BPD/94500/2013, IF/01420/2015, respectively).
publishDate 2019
dc.date.none.fl_str_mv 2019-10-16T10:53:53Z
2019-11
2019-10-07T10:35:53Z
2019-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/4213
url http://hdl.handle.net/10400.8/4213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro, H. R., Pestana, J. L. T., Soares, A. M. V. M., Novais, S. C., Lemos, M. F. L., Devreese, B., … Ramos, F. (2019). Assessment of fipronil toxicity to the freshwater midge Chironomus riparius: Molecular, biochemical, and organismal responses. Aquatic Toxicology, 216. https://doi.org/10.1016/j.aquatox.2019.105292
0166-445X
cv-prod-760979
10.1016/j.aquatox.2019.105292
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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