A silk fibroin hydrogel with reversible sol–gel transition

• Autor(a) principal: Yin, Zhuping
• Data de Publicação: 2017
• Outros Autores: Wu, Feng, Xing, Tieling, Yadavalli, Vamsi K., Kundu, Subhas C, Lu, Shenzhou
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/58190
• topic: Science & Technology

In this report, a novel silk fibroin hydrogel with a reversible sol-gel transition capacity is presented, in which the base material of this reversible hydrogel is hydrophilic silk fibroin (HSF) obtained by immersing a dried regenerated Bombyx mori silk fibroin (SF) condensate in deionized water (DI water) and collecting its lixivium. The resulting HSF sol can exhibit an enhanced sol-gel transition within several hours at suitable temperatures (25-50 degrees C) without any exterior additive, even at extremely low concentrations (<0.2%, w/v). The HSF gel can perform thixotropic, reversible gel-sol transitions triggered by a facile cycled shear-thinning and resting procedure. Reversible sol-gel transition kinetics analyses and dynamic measurements of the micro-structure transformation during the transition demonstrate that both the beta-sheet self-assembling process and metastable hydrogen bonding (H-bond) interactions among these large scale beta-sheet aggregates play essential roles in the significant enhancement of the reversible HSF sol-gel transition. Due to the reversible, thixotropic sol-gel transitions and suitable viscoelasticity of its shear-thinning system for matching random sizes/shapes, the HSF system is possibly an alternative injectable hydrogel for applications in 3-dimensional (3D) cell culture and tissue repair in situ.