The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6562 |
Resumo: | In the absence of viremia, the diagnostics of Zika virus (ZIKV) infections must rely on serologicaltechniques. InordertoimprovetheserologicaldiagnosisofZIKV,ZIKV-IgAandZIKV-IgG avidity assays were evaluated. Forty patients returning from ZIKV endemic areas, with confirmed or suspected ZIKV infections were studied. Samples were classified as early acute, acute and late acute according to the number of days post illness onset. Low avidity IgG was only detected at acute and late acute stages and IgA mostly at the early acute and acute stages. The date of sampling provides useful information and can help to choose the best technique to use at a determined moment in time and to interpret low avidity IgG and IgA results, improving the serological diagnosis of ZIKV. |
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The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus InfectionsZika VirusDengue VirusSecondary InfectionsCross-reactionsIgAIgG Avidity TestsInfecções Sistémicas e ZoonosesIn the absence of viremia, the diagnostics of Zika virus (ZIKV) infections must rely on serologicaltechniques. InordertoimprovetheserologicaldiagnosisofZIKV,ZIKV-IgAandZIKV-IgG avidity assays were evaluated. Forty patients returning from ZIKV endemic areas, with confirmed or suspected ZIKV infections were studied. Samples were classified as early acute, acute and late acute according to the number of days post illness onset. Low avidity IgG was only detected at acute and late acute stages and IgA mostly at the early acute and acute stages. The date of sampling provides useful information and can help to choose the best technique to use at a determined moment in time and to interpret low avidity IgG and IgA results, improving the serological diagnosis of ZIKV.This work has been partially funded by the ISCIII Project “PI16CIII/00037”.MDPIRepositório Científico do Instituto Nacional de SaúdeAmaro, F.Sánchez-Seco, M.P.Vásquez, A.Alves, M.J.Zé-Zé, L.Luz, M.T.Minguito, T.De La Fuente, JDe Ory, F.2020-04-30T17:59:47Z2019-02-202019-02-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6562engViruses. 2019 Feb 20;11(2):179. doi: 10.3390/v110201791999-491510.3390/v11020179info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:38Zoai:repositorio.insa.pt:10400.18/6562Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:26.920354Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
title |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
spellingShingle |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections Amaro, F. Zika Virus Dengue Virus Secondary Infections Cross-reactions IgA IgG Avidity Tests Infecções Sistémicas e Zoonoses |
title_short |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
title_full |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
title_fullStr |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
title_full_unstemmed |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
title_sort |
The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections |
author |
Amaro, F. |
author_facet |
Amaro, F. Sánchez-Seco, M.P. Vásquez, A. Alves, M.J. Zé-Zé, L. Luz, M.T. Minguito, T. De La Fuente, J De Ory, F. |
author_role |
author |
author2 |
Sánchez-Seco, M.P. Vásquez, A. Alves, M.J. Zé-Zé, L. Luz, M.T. Minguito, T. De La Fuente, J De Ory, F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Amaro, F. Sánchez-Seco, M.P. Vásquez, A. Alves, M.J. Zé-Zé, L. Luz, M.T. Minguito, T. De La Fuente, J De Ory, F. |
dc.subject.por.fl_str_mv |
Zika Virus Dengue Virus Secondary Infections Cross-reactions IgA IgG Avidity Tests Infecções Sistémicas e Zoonoses |
topic |
Zika Virus Dengue Virus Secondary Infections Cross-reactions IgA IgG Avidity Tests Infecções Sistémicas e Zoonoses |
description |
In the absence of viremia, the diagnostics of Zika virus (ZIKV) infections must rely on serologicaltechniques. InordertoimprovetheserologicaldiagnosisofZIKV,ZIKV-IgAandZIKV-IgG avidity assays were evaluated. Forty patients returning from ZIKV endemic areas, with confirmed or suspected ZIKV infections were studied. Samples were classified as early acute, acute and late acute according to the number of days post illness onset. Low avidity IgG was only detected at acute and late acute stages and IgA mostly at the early acute and acute stages. The date of sampling provides useful information and can help to choose the best technique to use at a determined moment in time and to interpret low avidity IgG and IgA results, improving the serological diagnosis of ZIKV. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02-20 2019-02-20T00:00:00Z 2020-04-30T17:59:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6562 |
url |
http://hdl.handle.net/10400.18/6562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Viruses. 2019 Feb 20;11(2):179. doi: 10.3390/v11020179 1999-4915 10.3390/v11020179 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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