Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Faustino, Margarida
Data de Publicação: 2022
Outros Autores: Durão, Joana, Pereira, Carla F., Oliveira, Ana Sofia, Pereira, Joana Odila, Pereira, Ana M., Ferreira, Carlos, Pintado, Manuela E., Carvalho, Ana P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/39485
Resumo: Mannans are outstanding polysaccharides that have gained exponential interest over the years. These polysaccharides may be extracted from the cell wall of Saccharomyces cerevisiae, and recovered from the brewing or synthetic biology industries, among others. In this work, several extraction processes—physical, chemical and enzymatic—were studied, all aiming to obtain mannans from spent yeast S. cerevisiae. Their performance was evaluated in terms of yield, mannose content and cost. The resultant extracts were characterized in terms of their structure (FT-IR, PXRD and SEM), physicochemical properties (color, molecular weight distribution, sugars, protein, ash and water content) and thermal stability (DSC). The biological properties were assessed through the screening of prebiotic activity in Lactobacillus plantarum and Bifidobacterium animalis. The highest yield (58.82%) was achieved by using an alkaline thermal process, though the correspondent mannose content was low. The extract obtained by autolysis followed by a hydrothermal step resulted in the highest mannose content (59.19%). On the other hand, the extract obtained through the enzymatic hydrolysis displayed the highest prebiotic activity. This comparative study is expected to lay the scientific foundation for the obtention of well-characterized mannans from yeast, which will pave the way for their application in various fields.
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spelling Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiaeMannansThermal hydrolysisAutolysisEnzymatic hydrolysisStructural characterizationPhysicochemical characterizationCost assessmentMannans are outstanding polysaccharides that have gained exponential interest over the years. These polysaccharides may be extracted from the cell wall of Saccharomyces cerevisiae, and recovered from the brewing or synthetic biology industries, among others. In this work, several extraction processes—physical, chemical and enzymatic—were studied, all aiming to obtain mannans from spent yeast S. cerevisiae. Their performance was evaluated in terms of yield, mannose content and cost. The resultant extracts were characterized in terms of their structure (FT-IR, PXRD and SEM), physicochemical properties (color, molecular weight distribution, sugars, protein, ash and water content) and thermal stability (DSC). The biological properties were assessed through the screening of prebiotic activity in Lactobacillus plantarum and Bifidobacterium animalis. The highest yield (58.82%) was achieved by using an alkaline thermal process, though the correspondent mannose content was low. The extract obtained by autolysis followed by a hydrothermal step resulted in the highest mannose content (59.19%). On the other hand, the extract obtained through the enzymatic hydrolysis displayed the highest prebiotic activity. This comparative study is expected to lay the scientific foundation for the obtention of well-characterized mannans from yeast, which will pave the way for their application in various fields.Veritati - Repositório Institucional da Universidade Católica PortuguesaFaustino, MargaridaDurão, JoanaPereira, Carla F.Oliveira, Ana SofiaPereira, Joana OdilaPereira, Ana M.Ferreira, CarlosPintado, Manuela E.Carvalho, Ana P.2022-12-06T15:28:31Z2022-112022-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/39485eng2304-815810.3390/foods1123375385143605579PMC973938936496561000897429400001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-09T01:36:35Zoai:repositorio.ucp.pt:10400.14/39485Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:32:19.273904Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
title Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
spellingShingle Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
Faustino, Margarida
Mannans
Thermal hydrolysis
Autolysis
Enzymatic hydrolysis
Structural characterization
Physicochemical characterization
Cost assessment
title_short Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
title_full Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
title_fullStr Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
title_full_unstemmed Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
title_sort Comparative analysis of mannans extraction processes from spent yeast saccharomyces cerevisiae
author Faustino, Margarida
author_facet Faustino, Margarida
Durão, Joana
Pereira, Carla F.
Oliveira, Ana Sofia
Pereira, Joana Odila
Pereira, Ana M.
Ferreira, Carlos
Pintado, Manuela E.
Carvalho, Ana P.
author_role author
author2 Durão, Joana
Pereira, Carla F.
Oliveira, Ana Sofia
Pereira, Joana Odila
Pereira, Ana M.
Ferreira, Carlos
Pintado, Manuela E.
Carvalho, Ana P.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Faustino, Margarida
Durão, Joana
Pereira, Carla F.
Oliveira, Ana Sofia
Pereira, Joana Odila
Pereira, Ana M.
Ferreira, Carlos
Pintado, Manuela E.
Carvalho, Ana P.
dc.subject.por.fl_str_mv Mannans
Thermal hydrolysis
Autolysis
Enzymatic hydrolysis
Structural characterization
Physicochemical characterization
Cost assessment
topic Mannans
Thermal hydrolysis
Autolysis
Enzymatic hydrolysis
Structural characterization
Physicochemical characterization
Cost assessment
description Mannans are outstanding polysaccharides that have gained exponential interest over the years. These polysaccharides may be extracted from the cell wall of Saccharomyces cerevisiae, and recovered from the brewing or synthetic biology industries, among others. In this work, several extraction processes—physical, chemical and enzymatic—were studied, all aiming to obtain mannans from spent yeast S. cerevisiae. Their performance was evaluated in terms of yield, mannose content and cost. The resultant extracts were characterized in terms of their structure (FT-IR, PXRD and SEM), physicochemical properties (color, molecular weight distribution, sugars, protein, ash and water content) and thermal stability (DSC). The biological properties were assessed through the screening of prebiotic activity in Lactobacillus plantarum and Bifidobacterium animalis. The highest yield (58.82%) was achieved by using an alkaline thermal process, though the correspondent mannose content was low. The extract obtained by autolysis followed by a hydrothermal step resulted in the highest mannose content (59.19%). On the other hand, the extract obtained through the enzymatic hydrolysis displayed the highest prebiotic activity. This comparative study is expected to lay the scientific foundation for the obtention of well-characterized mannans from yeast, which will pave the way for their application in various fields.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-06T15:28:31Z
2022-11
2022-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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url http://hdl.handle.net/10400.14/39485
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2304-8158
10.3390/foods11233753
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PMC9739389
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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