Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Luna, S. M.
Data de Publicação: 2010
Outros Autores: Gomes, Manuela E., Mano, J. F., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/20561
Resumo: Cells microencapsulated in biocompatible semi-permeable polymeric membranes are effective as cell delivery systems while protecting the host against immune responses. In this study, cell encapsulation membranes were prepared based on carrageenan and alginate, two natural cationic polymers. Different formulations/conditions were explored to optimize the microcapsules which were characterized with respect to their morphology, mechanical stability, and cytotoxicity. Spherical-shaped microcapsules were obtained from all the polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited the best stability and permeability, and therefore, these were selected for the cell encapsulation. These capsules provided an environment that supported cell proliferation and have the potential for tissue engineering as well as other cell-based therapy applications.
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spelling Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applicationsCapsulesCarrageenanAlginateFibroblastsCell viabilityScience & TechnologyCells microencapsulated in biocompatible semi-permeable polymeric membranes are effective as cell delivery systems while protecting the host against immune responses. In this study, cell encapsulation membranes were prepared based on carrageenan and alginate, two natural cationic polymers. Different formulations/conditions were explored to optimize the microcapsules which were characterized with respect to their morphology, mechanical stability, and cytotoxicity. Spherical-shaped microcapsules were obtained from all the polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited the best stability and permeability, and therefore, these were selected for the cell encapsulation. These capsules provided an environment that supported cell proliferation and have the potential for tissue engineering as well as other cell-based therapy applications.One of the authors (SML) acknowledges the support of the Programme Alssan-the European Union Programme of High Level Scholarships for Latin America (scholarship no. E04M041362CO). This work was partially supported by the European STREP HIPPOCRATES (NMP3-CT-2003-505758) and by the Fundacao para a Ciencia e Tecnologia (project PTDC/QUI/68804/2006) and carried out under the scope of European NoE EXPERTISSUES (NMP3-CT-2004-500283).SAGEUniversidade do MinhoLuna, S. M.Gomes, Manuela E.Mano, J. F.Reis, R. L.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20561eng0883-911510.1177/0883911510372173http://jbc.sagepub.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:31Zoai:repositorium.sdum.uminho.pt:1822/20561Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:39.411160Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
title Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
spellingShingle Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
Luna, S. M.
Capsules
Carrageenan
Alginate
Fibroblasts
Cell viability
Science & Technology
title_short Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
title_full Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
title_fullStr Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
title_full_unstemmed Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
title_sort Development of a novel cell encapsulation system based on natural origin polymers for tissue engineering applications
author Luna, S. M.
author_facet Luna, S. M.
Gomes, Manuela E.
Mano, J. F.
Reis, R. L.
author_role author
author2 Gomes, Manuela E.
Mano, J. F.
Reis, R. L.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Luna, S. M.
Gomes, Manuela E.
Mano, J. F.
Reis, R. L.
dc.subject.por.fl_str_mv Capsules
Carrageenan
Alginate
Fibroblasts
Cell viability
Science & Technology
topic Capsules
Carrageenan
Alginate
Fibroblasts
Cell viability
Science & Technology
description Cells microencapsulated in biocompatible semi-permeable polymeric membranes are effective as cell delivery systems while protecting the host against immune responses. In this study, cell encapsulation membranes were prepared based on carrageenan and alginate, two natural cationic polymers. Different formulations/conditions were explored to optimize the microcapsules which were characterized with respect to their morphology, mechanical stability, and cytotoxicity. Spherical-shaped microcapsules were obtained from all the polymeric systems. The iota-carrageenan/sodium alginate microcapsules exhibited the best stability and permeability, and therefore, these were selected for the cell encapsulation. These capsules provided an environment that supported cell proliferation and have the potential for tissue engineering as well as other cell-based therapy applications.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/20561
url http://hdl.handle.net/1822/20561
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0883-9115
10.1177/0883911510372173
http://jbc.sagepub.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SAGE
publisher.none.fl_str_mv SAGE
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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