Cell encapsulation in liquified compartments: Protocol optimization and challenges
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/28508 |
Resumo: | Cell encapsulation is a widely used technique in the field of Tissue Engineering and Regenerative Medicine (TERM). However, for the particular case of liquefied compartmentalised systems, only a limited number of studies have been reported in the literature. We have been exploring a unique cell encapsulation system composed by liquefied and multilayered capsules. This system transfigured the concept of 3D scaffolds for TERM, and was already successfully applied for bone and cartilage regeneration. Due to a number of appealing features, we envisage that it can be applied in many other fields, including in advanced therapies or as disease models for drug discovery. In this review, we intend to highlight the advantages of this new system, while discussing the methodology, and sharing the protocol optimization and results. The different liquefied systems for cell encapsulation reported in the literature will be also discussed, considering the different encapsulation matrixes as core templates, the types of membranes, and the core liquefaction treatments. |
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Cell encapsulation in liquified compartments: Protocol optimization and challengesAlginateLiquefied CapsulesCell EncapsulationTissue Engineering and Regenerative MedicineElectrosprayingMicroparticles3D Cell Culture SystemsHydrogelsLayer-by-LayerCell encapsulation is a widely used technique in the field of Tissue Engineering and Regenerative Medicine (TERM). However, for the particular case of liquefied compartmentalised systems, only a limited number of studies have been reported in the literature. We have been exploring a unique cell encapsulation system composed by liquefied and multilayered capsules. This system transfigured the concept of 3D scaffolds for TERM, and was already successfully applied for bone and cartilage regeneration. Due to a number of appealing features, we envisage that it can be applied in many other fields, including in advanced therapies or as disease models for drug discovery. In this review, we intend to highlight the advantages of this new system, while discussing the methodology, and sharing the protocol optimization and results. The different liquefied systems for cell encapsulation reported in the literature will be also discussed, considering the different encapsulation matrixes as core templates, the types of membranes, and the core liquefaction treatments.Public Library of Science2020-05-15T09:51:47Z2019-06-21T00:00:00Z2019-06-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/28508eng10.1371/journal.pone.0218045Correia, Clara R.Ghasemzadeh-Hasankolaei, MaryamMano, João F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:55:08Zoai:ria.ua.pt:10773/28508Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:01:01.985138Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
title |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
spellingShingle |
Cell encapsulation in liquified compartments: Protocol optimization and challenges Correia, Clara R. Alginate Liquefied Capsules Cell Encapsulation Tissue Engineering and Regenerative Medicine Electrospraying Microparticles 3D Cell Culture Systems Hydrogels Layer-by-Layer |
title_short |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
title_full |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
title_fullStr |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
title_full_unstemmed |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
title_sort |
Cell encapsulation in liquified compartments: Protocol optimization and challenges |
author |
Correia, Clara R. |
author_facet |
Correia, Clara R. Ghasemzadeh-Hasankolaei, Maryam Mano, João F. |
author_role |
author |
author2 |
Ghasemzadeh-Hasankolaei, Maryam Mano, João F. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Correia, Clara R. Ghasemzadeh-Hasankolaei, Maryam Mano, João F. |
dc.subject.por.fl_str_mv |
Alginate Liquefied Capsules Cell Encapsulation Tissue Engineering and Regenerative Medicine Electrospraying Microparticles 3D Cell Culture Systems Hydrogels Layer-by-Layer |
topic |
Alginate Liquefied Capsules Cell Encapsulation Tissue Engineering and Regenerative Medicine Electrospraying Microparticles 3D Cell Culture Systems Hydrogels Layer-by-Layer |
description |
Cell encapsulation is a widely used technique in the field of Tissue Engineering and Regenerative Medicine (TERM). However, for the particular case of liquefied compartmentalised systems, only a limited number of studies have been reported in the literature. We have been exploring a unique cell encapsulation system composed by liquefied and multilayered capsules. This system transfigured the concept of 3D scaffolds for TERM, and was already successfully applied for bone and cartilage regeneration. Due to a number of appealing features, we envisage that it can be applied in many other fields, including in advanced therapies or as disease models for drug discovery. In this review, we intend to highlight the advantages of this new system, while discussing the methodology, and sharing the protocol optimization and results. The different liquefied systems for cell encapsulation reported in the literature will be also discussed, considering the different encapsulation matrixes as core templates, the types of membranes, and the core liquefaction treatments. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-21T00:00:00Z 2019-06-21 2020-05-15T09:51:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/28508 |
url |
http://hdl.handle.net/10773/28508 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1371/journal.pone.0218045 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137666176909312 |