Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/114326 |
Resumo: | UIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymerChitin-glucan complex (CGC)Chitosan-glucan complex (ChGC)DissolutionNaOH/urea solvent systemsStructural analysisThermal propertiesBioengineeringUIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016Chitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first time for solubilization of CGC extracted from the yeast Komagataella pastoris. Different NaOH/urea ratios (6:8, 8:4 and 11:4 (w/w), respectively) were used to obtain aqueous solutions using a freeze/thaw procedure. There was an overall solubilization of 63–68%, with the highest solubilization rate obtained for the highest tested urea concentration (8 wt%). The regenerated polymer, obtained by dialysis of the alkali solutions followed by lyophilization, formed porous macrostructures characterized by a chemical composition similar to that of the starting co-polymer, although the acetylation degree decreased from 61.3% to 33.9–50.6%, indicating that chitin was converted into chitosan, yielding chitosan-glucan complex (ChGC). Consistent with this, there was a reduction of the crystallinity index and thermal degradation temperature. Given these results, this study reports a simple and green procedure to solubilize CGC and obtain aqueous ChGC solutions that can be processed as novel biomaterials.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisRUNAraújo, DianaAlves, Vítor D.Marques, Ana C.Fortunato, ElviraReis, Maria A. M.Freitas, Filomena2021-03-23T23:24:25Z2020-03-142020-03-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/114326eng2306-5354PURE: 18017744https://doi.org/10.3390/bioengineering7010028info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:51:20Zoai:run.unl.pt:10362/114326Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:51:20Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
title |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
spellingShingle |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer Araújo, Diana Chitin-glucan complex (CGC) Chitosan-glucan complex (ChGC) Dissolution NaOH/urea solvent systems Structural analysis Thermal properties Bioengineering |
title_short |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
title_full |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
title_fullStr |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
title_full_unstemmed |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
title_sort |
Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer |
author |
Araújo, Diana |
author_facet |
Araújo, Diana Alves, Vítor D. Marques, Ana C. Fortunato, Elvira Reis, Maria A. M. Freitas, Filomena |
author_role |
author |
author2 |
Alves, Vítor D. Marques, Ana C. Fortunato, Elvira Reis, Maria A. M. Freitas, Filomena |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais RUN |
dc.contributor.author.fl_str_mv |
Araújo, Diana Alves, Vítor D. Marques, Ana C. Fortunato, Elvira Reis, Maria A. M. Freitas, Filomena |
dc.subject.por.fl_str_mv |
Chitin-glucan complex (CGC) Chitosan-glucan complex (ChGC) Dissolution NaOH/urea solvent systems Structural analysis Thermal properties Bioengineering |
topic |
Chitin-glucan complex (CGC) Chitosan-glucan complex (ChGC) Dissolution NaOH/urea solvent systems Structural analysis Thermal properties Bioengineering |
description |
UIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016 |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-14 2020-03-14T00:00:00Z 2021-03-23T23:24:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/114326 |
url |
http://hdl.handle.net/10362/114326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2306-5354 PURE: 18017744 https://doi.org/10.3390/bioengineering7010028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817545786194395136 |