Depletion of the heaviest stable N isotope is associated with NH4 plants
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/3960 |
Resumo: | Open Access article - can be viewed online without a subscription at: http://dx.doi.org/doi:10.1186/1471-2229-11-83 |
| id |
RCAP_4f71ee6cd696cd2f60bd481c39be366a |
|---|---|
| oai_identifier_str |
oai:repositorio.ul.pt:10451/3960 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Depletion of the heaviest stable N isotope is associated with NH4 plantsLow affinity ammonium transportersNitrogen isotopic signatureAmmonium/ammoniaAmmonium dissociation isotope factorAmmonia uptakeOpen Access article - can be viewed online without a subscription at: http://dx.doi.org/doi:10.1186/1471-2229-11-83Background: In plants, nitrate (NO3 -) nutrition gives rise to a natural N isotopic signature (δ15N), which correlates with the δ15N of the N source. However, little is known about the relationship between the δ15N of the N source and the 14N/15N fractionation in plants under ammonium (NH4 +) nutrition. When NH4 + is the major N source, the two forms, NH4 + and NH3, are present in the nutrient solution. There is a 1.025 thermodynamic isotope effect between NH3 (g) and NH4 + (aq) which drives to a different δ15N. Nine plant species with different NH4 +-sensitivities were cultured hydroponically with NO3 - or NH4 + as the sole N sources, and plant growth and δ15N were determined. Short-term NH4 +/NH3 uptake experiments at pH 6.0 and 9.0 (which favours NH3 form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH4 + and NH3. Results: Several NO3 --fed plants were consistently enriched in 15N, whereas plants under NH4 + nutrition were depleted of 15N. It was shown that more sensitive plants to NH4 + toxicity were the most depleted in 15N. In parallel, N-deficient pea and spinach plants fed with 15NH4 + showed an increased level of NH3 uptake at alkaline pH that was related to the 15N depletion of the plant. Tolerant to NH4 + pea plants or sensitive spinach plants showed similar trend on 15N depletion while slight differences in the time kinetics were observed during the initial stages. The use of RbNO3 as control discarded that the differences observed arise from pH detrimental effects. Conclusions: This article proposes that the negative values of δ15N in NH4 +-fed plants are originated from NH3 uptake by plants. Moreover, this depletion of the heavier N isotope is proportional to the NH4 +/NH3 toxicity in plants species. Therefore, we hypothesise that the low affinity transport system for NH4 + may have two components: one that transports N in the molecular form and is associated with fractionation and another that transports N in the ionic form and is not associated with fractionationRepositório da Universidade de LisboaAriz, IdoiaCruz, CristinaMoran, Jose F.González-Moro, María B.García-Olaverri, CarmenGonzález-Murua, CarmenMartins-Loução, Maria A.Aparicio-Tejo, Pedro M2011-09-02T17:11:39Z2011-05-162011-05-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/3960engAriz et al. BMC Plant Biology 20111471-2229http://dx.doi.org/doi:10.1186/1471-2229-11-83info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:44:42Zoai:repositorio.ul.pt:10451/3960Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:29:45.364343Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| title |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| spellingShingle |
Depletion of the heaviest stable N isotope is associated with NH4 plants Ariz, Idoia Low affinity ammonium transporters Nitrogen isotopic signature Ammonium/ammonia Ammonium dissociation isotope factor Ammonia uptake |
| title_short |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| title_full |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| title_fullStr |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| title_full_unstemmed |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| title_sort |
Depletion of the heaviest stable N isotope is associated with NH4 plants |
| author |
Ariz, Idoia |
| author_facet |
Ariz, Idoia Cruz, Cristina Moran, Jose F. González-Moro, María B. García-Olaverri, Carmen González-Murua, Carmen Martins-Loução, Maria A. Aparicio-Tejo, Pedro M |
| author_role |
author |
| author2 |
Cruz, Cristina Moran, Jose F. González-Moro, María B. García-Olaverri, Carmen González-Murua, Carmen Martins-Loução, Maria A. Aparicio-Tejo, Pedro M |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Ariz, Idoia Cruz, Cristina Moran, Jose F. González-Moro, María B. García-Olaverri, Carmen González-Murua, Carmen Martins-Loução, Maria A. Aparicio-Tejo, Pedro M |
| dc.subject.por.fl_str_mv |
Low affinity ammonium transporters Nitrogen isotopic signature Ammonium/ammonia Ammonium dissociation isotope factor Ammonia uptake |
| topic |
Low affinity ammonium transporters Nitrogen isotopic signature Ammonium/ammonia Ammonium dissociation isotope factor Ammonia uptake |
| description |
Open Access article - can be viewed online without a subscription at: http://dx.doi.org/doi:10.1186/1471-2229-11-83 |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-09-02T17:11:39Z 2011-05-16 2011-05-16T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/3960 |
| url |
http://hdl.handle.net/10451/3960 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ariz et al. BMC Plant Biology 2011 1471-2229 http://dx.doi.org/doi:10.1186/1471-2229-11-83 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799134182969966592 |