Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/72563 |
Resumo: | Meiofauna organisms play an important role in ecological and sedimentary processes in estuarine ecosystems. Recently, the application of environmental DNA (eDNA) for investigating meiofauna in different environments, improved the accessibility to its diversity and composition in a scale, frequency and depth previously unattainable. Nevertheless, little attention has been given to the description of baseline patterns of coupled spatial and temporal dynamics of meiobenthic communities. In an earlier study conducted in Lima estuary (NW Portugal), using eDNA metabarcoding of sediment samples, high levels of meiofauna Operational Taxonomic Units (OTUs) turnover were recorded, between sampling points only a few metres apart, and among sampling stations along the estuary. In order to verify the consistency of these patterns, in the current study we re-assessed Lima estuary's meiofauna communities approximately 1 year after, applying the same methodological approach (targeting segments of the COI and 18S rRNA genes), and expanding HTS-data analyses through the use of association networks. A high degree of spatial turnover was found both within and between sampling stations and this was consistent for both markers and years. As a consequence, most of the beta-diversity was accounted by OTU replacement with only a minor contribution from OTU richness. Despite the high levels of OTU replacement, relatively stable network properties were found in meiofaunal communities, irrespective of the sampled year. Network properties appear to shift sharply from the downstream/high salinity area of the estuary to the mesohaline medium-upstream areas, suggesting high resilience and redundancy of meiofaunal communities along the estuarine gradient. The recognition of meiofauna's networks features may improve the understanding of the ecology and dynamics of these communities that apparently hold large portions of variable elements, thereby making difficult their analyses solely based on the OTU/species composition. |
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Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stabilityEstuarine ecosystemsMeiofaunaSpatial and temporal variationeDNA metabarcodingNetwork analysisCiências Naturais::Ciências BiológicasScience & TechnologyMeiofauna organisms play an important role in ecological and sedimentary processes in estuarine ecosystems. Recently, the application of environmental DNA (eDNA) for investigating meiofauna in different environments, improved the accessibility to its diversity and composition in a scale, frequency and depth previously unattainable. Nevertheless, little attention has been given to the description of baseline patterns of coupled spatial and temporal dynamics of meiobenthic communities. In an earlier study conducted in Lima estuary (NW Portugal), using eDNA metabarcoding of sediment samples, high levels of meiofauna Operational Taxonomic Units (OTUs) turnover were recorded, between sampling points only a few metres apart, and among sampling stations along the estuary. In order to verify the consistency of these patterns, in the current study we re-assessed Lima estuary's meiofauna communities approximately 1 year after, applying the same methodological approach (targeting segments of the COI and 18S rRNA genes), and expanding HTS-data analyses through the use of association networks. A high degree of spatial turnover was found both within and between sampling stations and this was consistent for both markers and years. As a consequence, most of the beta-diversity was accounted by OTU replacement with only a minor contribution from OTU richness. Despite the high levels of OTU replacement, relatively stable network properties were found in meiofaunal communities, irrespective of the sampled year. Network properties appear to shift sharply from the downstream/high salinity area of the estuary to the mesohaline medium-upstream areas, suggesting high resilience and redundancy of meiofaunal communities along the estuarine gradient. The recognition of meiofauna's networks features may improve the understanding of the ecology and dynamics of these communities that apparently hold large portions of variable elements, thereby making difficult their analyses solely based on the OTU/species composition.This study was funded by the project "The NextSea: Next generation monitoring of coastal ecosystems in a scenario of global change'' (operacao NORTE-01-0145-FEDER-000032), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Maria Fais and Sofia Duarte were supported, respectively by a Ph.D. (SFRH/BD/113547/2015) and a post-doc fellowship (SFRH/BPD/109842/2015) from FCT. The authors would like to thank Prof. Jesus Troncoso (University of Vigo) and Prof. Pedro Gomes (University of Minho) for their availability and hospitality for the lab processing; Dr. Bruno Sampieri and Barbara Leite (University of Minho) for the great support during the implementation of the practical stage of the research. The authors would like also to thank the anonymous reviewers for critically reading the manuscript and suggesting substantial improvements.ElsevierUniversidade do MinhoFais, M.Bellissario, B.Duarte, S.Vieira, P. E.Sousa, Ronaldo GomesCanchaya, C. A.Costa, Filipe O.20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/72563eng2352-485510.1016/j.rsma.2020.101341https://www.sciencedirect.com/science/article/pii/S2352485520304692info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:47Zoai:repositorium.sdum.uminho.pt:1822/72563Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:34.367595Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
title |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
spellingShingle |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability Fais, M. Estuarine ecosystems Meiofauna Spatial and temporal variation eDNA metabarcoding Network analysis Ciências Naturais::Ciências Biológicas Science & Technology |
title_short |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
title_full |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
title_fullStr |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
title_full_unstemmed |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
title_sort |
Meiofauna metabarcoding in Lima estuary (Portugal) suggests high taxon replacement within a background of network stability |
author |
Fais, M. |
author_facet |
Fais, M. Bellissario, B. Duarte, S. Vieira, P. E. Sousa, Ronaldo Gomes Canchaya, C. A. Costa, Filipe O. |
author_role |
author |
author2 |
Bellissario, B. Duarte, S. Vieira, P. E. Sousa, Ronaldo Gomes Canchaya, C. A. Costa, Filipe O. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fais, M. Bellissario, B. Duarte, S. Vieira, P. E. Sousa, Ronaldo Gomes Canchaya, C. A. Costa, Filipe O. |
dc.subject.por.fl_str_mv |
Estuarine ecosystems Meiofauna Spatial and temporal variation eDNA metabarcoding Network analysis Ciências Naturais::Ciências Biológicas Science & Technology |
topic |
Estuarine ecosystems Meiofauna Spatial and temporal variation eDNA metabarcoding Network analysis Ciências Naturais::Ciências Biológicas Science & Technology |
description |
Meiofauna organisms play an important role in ecological and sedimentary processes in estuarine ecosystems. Recently, the application of environmental DNA (eDNA) for investigating meiofauna in different environments, improved the accessibility to its diversity and composition in a scale, frequency and depth previously unattainable. Nevertheless, little attention has been given to the description of baseline patterns of coupled spatial and temporal dynamics of meiobenthic communities. In an earlier study conducted in Lima estuary (NW Portugal), using eDNA metabarcoding of sediment samples, high levels of meiofauna Operational Taxonomic Units (OTUs) turnover were recorded, between sampling points only a few metres apart, and among sampling stations along the estuary. In order to verify the consistency of these patterns, in the current study we re-assessed Lima estuary's meiofauna communities approximately 1 year after, applying the same methodological approach (targeting segments of the COI and 18S rRNA genes), and expanding HTS-data analyses through the use of association networks. A high degree of spatial turnover was found both within and between sampling stations and this was consistent for both markers and years. As a consequence, most of the beta-diversity was accounted by OTU replacement with only a minor contribution from OTU richness. Despite the high levels of OTU replacement, relatively stable network properties were found in meiofaunal communities, irrespective of the sampled year. Network properties appear to shift sharply from the downstream/high salinity area of the estuary to the mesohaline medium-upstream areas, suggesting high resilience and redundancy of meiofaunal communities along the estuarine gradient. The recognition of meiofauna's networks features may improve the understanding of the ecology and dynamics of these communities that apparently hold large portions of variable elements, thereby making difficult their analyses solely based on the OTU/species composition. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/72563 |
url |
http://hdl.handle.net/1822/72563 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2352-4855 10.1016/j.rsma.2020.101341 https://www.sciencedirect.com/science/article/pii/S2352485520304692 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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