Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages

Detalhes bibliográficos
Autor(a) principal: Castro, Vânia Isabel Baptista
Data de Publicação: 2023
Outros Autores: Araújo, Ana Rita Rodrigues, Duarte, Filipa, Sousa-Franco, António, Reis, R. L., Pashkuleva, I., Pires, R. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/85090
Resumo: We applied a bottom-up approach to develop biofunctional supramolecular hydrogels from an aromatic glycodipeptide. The self-assembly of the glycopeptide was induced by either temperature manipulation (heatingâ cooling cycle) or solvent (DMSO to water) switch. The solâ gel transition was salt-triggered in cell culture media and resulted in gels with the same chemical compositions but different mechanical properties. Human adipose derived stem cells (hASCs) cultured on these gels under basal conditions (i.e., without differentiation factors) overexpressed neural markers, such as GFAP, Nestin, MAP2, and βIII-tubulin, confirming the differentiation into neural lineages. The mechanical properties of the gels influenced the number and distribution of the adhered cells. A comparison with gels obtained from the nonglycosylated peptide showed that glycosylation is crucial for the biofunctionality of the hydrogels by capturing and preserving essential growth factors...
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spelling Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineagesGlycopeptidePlycopeptideNeural tissueStem cells differentiationSupramolecularHydrogelsstem cell differentiationWe applied a bottom-up approach to develop biofunctional supramolecular hydrogels from an aromatic glycodipeptide. The self-assembly of the glycopeptide was induced by either temperature manipulation (heatingâ cooling cycle) or solvent (DMSO to water) switch. The solâ gel transition was salt-triggered in cell culture media and resulted in gels with the same chemical compositions but different mechanical properties. Human adipose derived stem cells (hASCs) cultured on these gels under basal conditions (i.e., without differentiation factors) overexpressed neural markers, such as GFAP, Nestin, MAP2, and βIII-tubulin, confirming the differentiation into neural lineages. The mechanical properties of the gels influenced the number and distribution of the adhered cells. A comparison with gels obtained from the nonglycosylated peptide showed that glycosylation is crucial for the biofunctionality of the hydrogels by capturing and preserving essential growth factors...We acknowledge the Portuguese Foundation for Science and Technology (FCT, RAP-CEECIND/05623/2022, VIBC-PD/BD/135256/2017; COVID/BD/152018/2021) for the financial support.ACS PublicationsUniversidade do MinhoCastro, Vânia Isabel BaptistaAraújo, Ana Rita RodriguesDuarte, FilipaSousa-Franco, AntónioReis, R. L.Pashkuleva, I.Pires, R. A.2023-062023-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85090engCastro V. I. B., Araújo A. R., Duarte F., Sousa-Franco A., Reis R. L., Pashkuleva I., Pires R. A. Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages, Acs Applied Materials & Interfaces, doi:10.1021/acsami.3c05309, 20231944-824410.1021/acsami.3c0530937327399https://doi.org/10.1021/acsami.3c05309info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:17:42Zoai:repositorium.sdum.uminho.pt:1822/85090Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:14.135340Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
title Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
spellingShingle Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
Castro, Vânia Isabel Baptista
Glycopeptide
Plycopeptide
Neural tissue
Stem cells differentiation
Supramolecular
Hydrogels
stem cell differentiation
title_short Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
title_full Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
title_fullStr Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
title_full_unstemmed Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
title_sort Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages
author Castro, Vânia Isabel Baptista
author_facet Castro, Vânia Isabel Baptista
Araújo, Ana Rita Rodrigues
Duarte, Filipa
Sousa-Franco, António
Reis, R. L.
Pashkuleva, I.
Pires, R. A.
author_role author
author2 Araújo, Ana Rita Rodrigues
Duarte, Filipa
Sousa-Franco, António
Reis, R. L.
Pashkuleva, I.
Pires, R. A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Castro, Vânia Isabel Baptista
Araújo, Ana Rita Rodrigues
Duarte, Filipa
Sousa-Franco, António
Reis, R. L.
Pashkuleva, I.
Pires, R. A.
dc.subject.por.fl_str_mv Glycopeptide
Plycopeptide
Neural tissue
Stem cells differentiation
Supramolecular
Hydrogels
stem cell differentiation
topic Glycopeptide
Plycopeptide
Neural tissue
Stem cells differentiation
Supramolecular
Hydrogels
stem cell differentiation
description We applied a bottom-up approach to develop biofunctional supramolecular hydrogels from an aromatic glycodipeptide. The self-assembly of the glycopeptide was induced by either temperature manipulation (heatingâ cooling cycle) or solvent (DMSO to water) switch. The solâ gel transition was salt-triggered in cell culture media and resulted in gels with the same chemical compositions but different mechanical properties. Human adipose derived stem cells (hASCs) cultured on these gels under basal conditions (i.e., without differentiation factors) overexpressed neural markers, such as GFAP, Nestin, MAP2, and βIII-tubulin, confirming the differentiation into neural lineages. The mechanical properties of the gels influenced the number and distribution of the adhered cells. A comparison with gels obtained from the nonglycosylated peptide showed that glycosylation is crucial for the biofunctionality of the hydrogels by capturing and preserving essential growth factors...
publishDate 2023
dc.date.none.fl_str_mv 2023-06
2023-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/85090
url https://hdl.handle.net/1822/85090
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castro V. I. B., Araújo A. R., Duarte F., Sousa-Franco A., Reis R. L., Pashkuleva I., Pires R. A. Glycopeptide-based supramolecular hydrogels induce differentiation of adipose stem cells into neural lineages, Acs Applied Materials & Interfaces, doi:10.1021/acsami.3c05309, 2023
1944-8244
10.1021/acsami.3c05309
37327399
https://doi.org/10.1021/acsami.3c05309
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132805006884864

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