Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes

Detalhes bibliográficos
Autor(a) principal: Carvalho, Pablo Herthel
Data de Publicação: 2012
Outros Autores: Daibert, Ana Paula Falci, Monteiro, Betânia Souza, Okano, Bárbara Silva, Carvalho, Juliana Lott, Cunha, Daise Nunes Queiroz da, Favarato, Lukiya Silva Campos, Pereira, Vanessa Guedes, Augusto, Luis Eugênio Franklin, Del Carlo, Ricardo Junqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S0066-782X2012005000114
http://www.locus.ufv.br/handle/123456789/12120
Resumo: Cardiomyocytes have small potential for renovation and proliferation in vivo. Consequently, the heart muscle has limited capacity of self-renewal. Mesenchymal stem cells (MSC) therapy, as well as MSC differentiated into cardiomyocytes, has been used in the attempt to minimize the effects of ischemic-hypoxic lesions and those affecting the electrical conduction system of the heart. The present study compared three distinct protocols for induced differentiation of MSC into cardiomyocytes aimed at finding a viable method for producing a large number of functional cells expressing cardiomyogenic phenotype. Mesenchymal stem cells were obtained from the adipose tissue of young transgenic Lewis rats expressing green fluorescent protein (GFP), and submitted to three distinct differentiation-inducing media: 1) Planat-Bérnard, 2) 5-azacytidine, and 3) Planat-Bérnard + 5-azacytidine; further, these cells were identified based on the expression of cardiac cell markers. All three protocols detected the expression of sarcomeric-alpha-actinin protein in the exoskeleton of cells, expression of connexin-43 in the nuclear and cytoplasmic membrane, and formation of gap junctions, which are necessary for electrical impulse propagation in the myocardium. However, no spontaneous cell contraction was observed with any of the tested protocols. Induction with 5-azacytidine provided an effective cadiomyogenic cellular differentiation similar to that obtained with Planat-Bénard media. Therefore, 5-azacytidine was the method of choice for being the simplest, fastest and lowest-cost protocol for cell differentiation. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0)
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spelling Carvalho, Pablo HerthelDaibert, Ana Paula FalciMonteiro, Betânia SouzaOkano, Bárbara SilvaCarvalho, Juliana LottCunha, Daise Nunes Queiroz daFavarato, Lukiya Silva CamposPereira, Vanessa GuedesAugusto, Luis Eugênio FranklinDel Carlo, Ricardo Junqueira2017-10-18T11:15:19Z2017-10-18T11:15:19Z2012-12-110066-782Xhttp://dx.doi.org/10.1590/S0066-782X2012005000114http://www.locus.ufv.br/handle/123456789/12120Cardiomyocytes have small potential for renovation and proliferation in vivo. Consequently, the heart muscle has limited capacity of self-renewal. Mesenchymal stem cells (MSC) therapy, as well as MSC differentiated into cardiomyocytes, has been used in the attempt to minimize the effects of ischemic-hypoxic lesions and those affecting the electrical conduction system of the heart. The present study compared three distinct protocols for induced differentiation of MSC into cardiomyocytes aimed at finding a viable method for producing a large number of functional cells expressing cardiomyogenic phenotype. Mesenchymal stem cells were obtained from the adipose tissue of young transgenic Lewis rats expressing green fluorescent protein (GFP), and submitted to three distinct differentiation-inducing media: 1) Planat-Bérnard, 2) 5-azacytidine, and 3) Planat-Bérnard + 5-azacytidine; further, these cells were identified based on the expression of cardiac cell markers. All three protocols detected the expression of sarcomeric-alpha-actinin protein in the exoskeleton of cells, expression of connexin-43 in the nuclear and cytoplasmic membrane, and formation of gap junctions, which are necessary for electrical impulse propagation in the myocardium. However, no spontaneous cell contraction was observed with any of the tested protocols. Induction with 5-azacytidine provided an effective cadiomyogenic cellular differentiation similar to that obtained with Planat-Bénard media. Therefore, 5-azacytidine was the method of choice for being the simplest, fastest and lowest-cost protocol for cell differentiation. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0)engArquivos Brasileiros de Cardiologiavol. 100 n. 1 p. 82-89 dez. 2012Somatic stem cells5-azacytidineCellular therapyCardiomyocytesCell differentiationDifferentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALen_aop11412.pdfen_aop11412.pdftexto completoapplication/pdf1809023https://locus.ufv.br//bitstream/123456789/12120/1/en_aop11412.pdf2c768f594f35779e7d579109ee3c0793MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12120/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILen_aop11412.pdf.jpgen_aop11412.pdf.jpgIM Thumbnailimage/jpeg4715https://locus.ufv.br//bitstream/123456789/12120/3/en_aop11412.pdf.jpg83be1b85b0811b378710e69616686424MD53123456789/121202017-10-18 22:00:57.508oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-19T01:00:57LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
title Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
spellingShingle Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
Carvalho, Pablo Herthel
Somatic stem cells
5-azacytidine
Cellular therapy
Cardiomyocytes
Cell differentiation
title_short Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
title_full Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
title_fullStr Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
title_full_unstemmed Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
title_sort Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
author Carvalho, Pablo Herthel
author_facet Carvalho, Pablo Herthel
Daibert, Ana Paula Falci
Monteiro, Betânia Souza
Okano, Bárbara Silva
Carvalho, Juliana Lott
Cunha, Daise Nunes Queiroz da
Favarato, Lukiya Silva Campos
Pereira, Vanessa Guedes
Augusto, Luis Eugênio Franklin
Del Carlo, Ricardo Junqueira
author_role author
author2 Daibert, Ana Paula Falci
Monteiro, Betânia Souza
Okano, Bárbara Silva
Carvalho, Juliana Lott
Cunha, Daise Nunes Queiroz da
Favarato, Lukiya Silva Campos
Pereira, Vanessa Guedes
Augusto, Luis Eugênio Franklin
Del Carlo, Ricardo Junqueira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Pablo Herthel
Daibert, Ana Paula Falci
Monteiro, Betânia Souza
Okano, Bárbara Silva
Carvalho, Juliana Lott
Cunha, Daise Nunes Queiroz da
Favarato, Lukiya Silva Campos
Pereira, Vanessa Guedes
Augusto, Luis Eugênio Franklin
Del Carlo, Ricardo Junqueira
dc.subject.pt-BR.fl_str_mv Somatic stem cells
5-azacytidine
Cellular therapy
Cardiomyocytes
Cell differentiation
topic Somatic stem cells
5-azacytidine
Cellular therapy
Cardiomyocytes
Cell differentiation
description Cardiomyocytes have small potential for renovation and proliferation in vivo. Consequently, the heart muscle has limited capacity of self-renewal. Mesenchymal stem cells (MSC) therapy, as well as MSC differentiated into cardiomyocytes, has been used in the attempt to minimize the effects of ischemic-hypoxic lesions and those affecting the electrical conduction system of the heart. The present study compared three distinct protocols for induced differentiation of MSC into cardiomyocytes aimed at finding a viable method for producing a large number of functional cells expressing cardiomyogenic phenotype. Mesenchymal stem cells were obtained from the adipose tissue of young transgenic Lewis rats expressing green fluorescent protein (GFP), and submitted to three distinct differentiation-inducing media: 1) Planat-Bérnard, 2) 5-azacytidine, and 3) Planat-Bérnard + 5-azacytidine; further, these cells were identified based on the expression of cardiac cell markers. All three protocols detected the expression of sarcomeric-alpha-actinin protein in the exoskeleton of cells, expression of connexin-43 in the nuclear and cytoplasmic membrane, and formation of gap junctions, which are necessary for electrical impulse propagation in the myocardium. However, no spontaneous cell contraction was observed with any of the tested protocols. Induction with 5-azacytidine provided an effective cadiomyogenic cellular differentiation similar to that obtained with Planat-Bénard media. Therefore, 5-azacytidine was the method of choice for being the simplest, fastest and lowest-cost protocol for cell differentiation. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0)
publishDate 2012
dc.date.issued.fl_str_mv 2012-12-11
dc.date.accessioned.fl_str_mv 2017-10-18T11:15:19Z
dc.date.available.fl_str_mv 2017-10-18T11:15:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0066-782X2012005000114
http://www.locus.ufv.br/handle/123456789/12120
dc.identifier.issn.none.fl_str_mv 0066-782X
identifier_str_mv 0066-782X
url http://dx.doi.org/10.1590/S0066-782X2012005000114
http://www.locus.ufv.br/handle/123456789/12120
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv vol. 100 n. 1 p. 82-89 dez. 2012
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dc.publisher.none.fl_str_mv Arquivos Brasileiros de Cardiologia
publisher.none.fl_str_mv Arquivos Brasileiros de Cardiologia
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