Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/S0066-782X2012005000114 http://www.locus.ufv.br/handle/123456789/12120 |
Resumo: | Cardiomyocytes have small potential for renovation and proliferation in vivo. Consequently, the heart muscle has limited capacity of self-renewal. Mesenchymal stem cells (MSC) therapy, as well as MSC differentiated into cardiomyocytes, has been used in the attempt to minimize the effects of ischemic-hypoxic lesions and those affecting the electrical conduction system of the heart. The present study compared three distinct protocols for induced differentiation of MSC into cardiomyocytes aimed at finding a viable method for producing a large number of functional cells expressing cardiomyogenic phenotype. Mesenchymal stem cells were obtained from the adipose tissue of young transgenic Lewis rats expressing green fluorescent protein (GFP), and submitted to three distinct differentiation-inducing media: 1) Planat-Bérnard, 2) 5-azacytidine, and 3) Planat-Bérnard + 5-azacytidine; further, these cells were identified based on the expression of cardiac cell markers. All three protocols detected the expression of sarcomeric-alpha-actinin protein in the exoskeleton of cells, expression of connexin-43 in the nuclear and cytoplasmic membrane, and formation of gap junctions, which are necessary for electrical impulse propagation in the myocardium. However, no spontaneous cell contraction was observed with any of the tested protocols. Induction with 5-azacytidine provided an effective cadiomyogenic cellular differentiation similar to that obtained with Planat-Bénard media. Therefore, 5-azacytidine was the method of choice for being the simplest, fastest and lowest-cost protocol for cell differentiation. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0) |
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Carvalho, Pablo HerthelDaibert, Ana Paula FalciMonteiro, Betânia SouzaOkano, Bárbara SilvaCarvalho, Juliana LottCunha, Daise Nunes Queiroz daFavarato, Lukiya Silva CamposPereira, Vanessa GuedesAugusto, Luis Eugênio FranklinDel Carlo, Ricardo Junqueira2017-10-18T11:15:19Z2017-10-18T11:15:19Z2012-12-110066-782Xhttp://dx.doi.org/10.1590/S0066-782X2012005000114http://www.locus.ufv.br/handle/123456789/12120Cardiomyocytes have small potential for renovation and proliferation in vivo. Consequently, the heart muscle has limited capacity of self-renewal. Mesenchymal stem cells (MSC) therapy, as well as MSC differentiated into cardiomyocytes, has been used in the attempt to minimize the effects of ischemic-hypoxic lesions and those affecting the electrical conduction system of the heart. The present study compared three distinct protocols for induced differentiation of MSC into cardiomyocytes aimed at finding a viable method for producing a large number of functional cells expressing cardiomyogenic phenotype. Mesenchymal stem cells were obtained from the adipose tissue of young transgenic Lewis rats expressing green fluorescent protein (GFP), and submitted to three distinct differentiation-inducing media: 1) Planat-Bérnard, 2) 5-azacytidine, and 3) Planat-Bérnard + 5-azacytidine; further, these cells were identified based on the expression of cardiac cell markers. All three protocols detected the expression of sarcomeric-alpha-actinin protein in the exoskeleton of cells, expression of connexin-43 in the nuclear and cytoplasmic membrane, and formation of gap junctions, which are necessary for electrical impulse propagation in the myocardium. However, no spontaneous cell contraction was observed with any of the tested protocols. Induction with 5-azacytidine provided an effective cadiomyogenic cellular differentiation similar to that obtained with Planat-Bénard media. Therefore, 5-azacytidine was the method of choice for being the simplest, fastest and lowest-cost protocol for cell differentiation. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0)engArquivos Brasileiros de Cardiologiavol. 100 n. 1 p. 82-89 dez. 2012Somatic stem cells5-azacytidineCellular therapyCardiomyocytesCell differentiationDifferentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALen_aop11412.pdfen_aop11412.pdftexto completoapplication/pdf1809023https://locus.ufv.br//bitstream/123456789/12120/1/en_aop11412.pdf2c768f594f35779e7d579109ee3c0793MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12120/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILen_aop11412.pdf.jpgen_aop11412.pdf.jpgIM Thumbnailimage/jpeg4715https://locus.ufv.br//bitstream/123456789/12120/3/en_aop11412.pdf.jpg83be1b85b0811b378710e69616686424MD53123456789/121202017-10-18 22:00:57.508oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-19T01:00:57LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
title |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
spellingShingle |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes Carvalho, Pablo Herthel Somatic stem cells 5-azacytidine Cellular therapy Cardiomyocytes Cell differentiation |
title_short |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
title_full |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
title_fullStr |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
title_full_unstemmed |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
title_sort |
Differentiation of adipose tissue-derived mesenchymal stem cells into cardiomyocytes |
author |
Carvalho, Pablo Herthel |
author_facet |
Carvalho, Pablo Herthel Daibert, Ana Paula Falci Monteiro, Betânia Souza Okano, Bárbara Silva Carvalho, Juliana Lott Cunha, Daise Nunes Queiroz da Favarato, Lukiya Silva Campos Pereira, Vanessa Guedes Augusto, Luis Eugênio Franklin Del Carlo, Ricardo Junqueira |
author_role |
author |
author2 |
Daibert, Ana Paula Falci Monteiro, Betânia Souza Okano, Bárbara Silva Carvalho, Juliana Lott Cunha, Daise Nunes Queiroz da Favarato, Lukiya Silva Campos Pereira, Vanessa Guedes Augusto, Luis Eugênio Franklin Del Carlo, Ricardo Junqueira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Pablo Herthel Daibert, Ana Paula Falci Monteiro, Betânia Souza Okano, Bárbara Silva Carvalho, Juliana Lott Cunha, Daise Nunes Queiroz da Favarato, Lukiya Silva Campos Pereira, Vanessa Guedes Augusto, Luis Eugênio Franklin Del Carlo, Ricardo Junqueira |
dc.subject.pt-BR.fl_str_mv |
Somatic stem cells 5-azacytidine Cellular therapy Cardiomyocytes Cell differentiation |
topic |
Somatic stem cells 5-azacytidine Cellular therapy Cardiomyocytes Cell differentiation |
description |
Cardiomyocytes have small potential for renovation and proliferation in vivo. Consequently, the heart muscle has limited capacity of self-renewal. Mesenchymal stem cells (MSC) therapy, as well as MSC differentiated into cardiomyocytes, has been used in the attempt to minimize the effects of ischemic-hypoxic lesions and those affecting the electrical conduction system of the heart. The present study compared three distinct protocols for induced differentiation of MSC into cardiomyocytes aimed at finding a viable method for producing a large number of functional cells expressing cardiomyogenic phenotype. Mesenchymal stem cells were obtained from the adipose tissue of young transgenic Lewis rats expressing green fluorescent protein (GFP), and submitted to three distinct differentiation-inducing media: 1) Planat-Bérnard, 2) 5-azacytidine, and 3) Planat-Bérnard + 5-azacytidine; further, these cells were identified based on the expression of cardiac cell markers. All three protocols detected the expression of sarcomeric-alpha-actinin protein in the exoskeleton of cells, expression of connexin-43 in the nuclear and cytoplasmic membrane, and formation of gap junctions, which are necessary for electrical impulse propagation in the myocardium. However, no spontaneous cell contraction was observed with any of the tested protocols. Induction with 5-azacytidine provided an effective cadiomyogenic cellular differentiation similar to that obtained with Planat-Bénard media. Therefore, 5-azacytidine was the method of choice for being the simplest, fastest and lowest-cost protocol for cell differentiation. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0) |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-12-11 |
dc.date.accessioned.fl_str_mv |
2017-10-18T11:15:19Z |
dc.date.available.fl_str_mv |
2017-10-18T11:15:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0066-782X2012005000114 http://www.locus.ufv.br/handle/123456789/12120 |
dc.identifier.issn.none.fl_str_mv |
0066-782X |
identifier_str_mv |
0066-782X |
url |
http://dx.doi.org/10.1590/S0066-782X2012005000114 http://www.locus.ufv.br/handle/123456789/12120 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
vol. 100 n. 1 p. 82-89 dez. 2012 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Arquivos Brasileiros de Cardiologia |
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Arquivos Brasileiros de Cardiologia |
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