Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4

Detalhes bibliográficos
Autor(a) principal: Venâncio, Armando
Data de Publicação: 1999
Outros Autores: Domingues, Lucília, Lima, Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1399
Resumo: A flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single-stranded carrier DNA using the shuttle vector pYAC4. The transformation efficiency was above 10³ transformants per microgram of plasmid DNA which is similar to other described yeast transformation systems. Under selective pressure, the transformed cells were stable and maintained the flocculation ability. Thus, this simple transformation system can be used for gene expression studies in flocculating yeasts, overcoming disadvantages of conventional methods such as the spheroplast one.
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spelling Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4Science & TechnologyA flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single-stranded carrier DNA using the shuttle vector pYAC4. The transformation efficiency was above 10³ transformants per microgram of plasmid DNA which is similar to other described yeast transformation systems. Under selective pressure, the transformed cells were stable and maintained the flocculation ability. Thus, this simple transformation system can be used for gene expression studies in flocculating yeasts, overcoming disadvantages of conventional methods such as the spheroplast one.Fundação para a Ciência e Tecnologia (FCT) - PRAXIS XXI/BD/11306/97WileyUniversidade do MinhoVenâncio, ArmandoDomingues, LucíliaLima, Nelson19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1399eng"Journal of basic microbiology". ISSN 0233-111X. 39:1 (1999) 37-41.0233-111X10071865info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:16:32Zoai:repositorium.sdum.uminho.pt:1822/1399Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:04.785645Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
title Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
spellingShingle Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
Venâncio, Armando
Science & Technology
title_short Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
title_full Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
title_fullStr Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
title_full_unstemmed Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
title_sort Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
author Venâncio, Armando
author_facet Venâncio, Armando
Domingues, Lucília
Lima, Nelson
author_role author
author2 Domingues, Lucília
Lima, Nelson
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Venâncio, Armando
Domingues, Lucília
Lima, Nelson
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description A flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single-stranded carrier DNA using the shuttle vector pYAC4. The transformation efficiency was above 10³ transformants per microgram of plasmid DNA which is similar to other described yeast transformation systems. Under selective pressure, the transformed cells were stable and maintained the flocculation ability. Thus, this simple transformation system can be used for gene expression studies in flocculating yeasts, overcoming disadvantages of conventional methods such as the spheroplast one.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1399
url http://hdl.handle.net/1822/1399
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of basic microbiology". ISSN 0233-111X. 39:1 (1999) 37-41.
0233-111X
10071865
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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