Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain

Detalhes bibliográficos
Autor(a) principal: Domingues, Lucília
Data de Publicação: 1999
Outros Autores: Dantas, Maria M., Lima, Nelson, Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1417
Resumo: Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L−1 h−1 (corresponding to a feed lactose concentration of 50 g L−1 and a dilution rate of 0.55 h−1) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months.
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spelling Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strainLactose fermentationRecombinant yeastEthanol productionContinuous cultureFlocculationAirliftScience & TechnologyAlcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L−1 h−1 (corresponding to a feed lactose concentration of 50 g L−1 and a dilution rate of 0.55 h−1) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months.Instituto de Biotecnologia e Química Fina (IBQF) - Fundação para a Ciência e a Tecnologia (FCT) - PRAXISXXI/BD/11306/97John Wiley and SonsUniversidade do MinhoDomingues, LucíliaDantas, Maria M.Lima, NelsonTeixeira, J. A.1999-091999-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1417eng“Biotechnology and bioengineering”. ISSN 0006-3592. 64:6 (1999) 692-697.0006-359210.1002/(SICI)1097-0290(19990920)64:6<692::AID-BIT8>3.0.CO;2-J10417218info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:29:03Zoai:repositorium.sdum.uminho.pt:1822/1417Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:29:03Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
title Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
spellingShingle Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
Domingues, Lucília
Lactose fermentation
Recombinant yeast
Ethanol production
Continuous culture
Flocculation
Airlift
Science & Technology
title_short Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
title_full Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
title_fullStr Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
title_full_unstemmed Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
title_sort Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain
author Domingues, Lucília
author_facet Domingues, Lucília
Dantas, Maria M.
Lima, Nelson
Teixeira, J. A.
author_role author
author2 Dantas, Maria M.
Lima, Nelson
Teixeira, J. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Domingues, Lucília
Dantas, Maria M.
Lima, Nelson
Teixeira, J. A.
dc.subject.por.fl_str_mv Lactose fermentation
Recombinant yeast
Ethanol production
Continuous culture
Flocculation
Airlift
Science & Technology
topic Lactose fermentation
Recombinant yeast
Ethanol production
Continuous culture
Flocculation
Airlift
Science & Technology
description Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L−1 h−1 (corresponding to a feed lactose concentration of 50 g L−1 and a dilution rate of 0.55 h−1) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months.
publishDate 1999
dc.date.none.fl_str_mv 1999-09
1999-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1417
url http://hdl.handle.net/1822/1417
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Biotechnology and bioengineering”. ISSN 0006-3592. 64:6 (1999) 692-697.
0006-3592
10.1002/(SICI)1097-0290(19990920)64:6<692::AID-BIT8>3.0.CO;2-J
10417218
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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