Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family

Detalhes bibliográficos
Autor(a) principal: Mestre, Olga Maria Elviro, 1984-
Data de Publicação: 2007
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1188
Resumo: Tese de mestrado em Biologia Molecular e Genética apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2007
id RCAP_510b741e43a05e1e39996cb3a1d4c44f
oai_identifier_str oai:repositorio.ul.pt:10451/1188
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Genetic diversity in Mycobacterium tuberculosis: the Beijing/W familyMycobacterium tuberculosisGenética microbianaMycobacterium tuberculosisTeses de MestradoTese de mestrado em Biologia Molecular e Genética apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2007The Beijing/W family of Mycobacterium tuberculosis represents a global threat in tuberculosis control. It has been frequently associated with drug resistance and its wide distribution suggests that these strains may have selective advantages over others. On the present study a collection of Beijing/W strains isolated in different countries was analyzed under the genetic markers: Large Sequence Polymorphisms (LSPs) and Single Nucleotide Polymorphisms (SNPs). Through a Multiplex-PCR isolates were tested for the presence or absence of the LSPs, described by a previous study for the Beijing/W family (RD105, RD181, RD150, RD142). The analysis of these regions proved to be very useful for defining and broadly subdivide the Beijing/W family in four groups. Furthermore, it provided a support for selection of representative isolates for the further study of other markers like SNPs in recently identified polymorphic DNA repair, recombination and replication (3R) genes. Selected 3R genes were sequenced for representative Beijing/W isolates to search for variations and comparative analysis with M.tuberculosis H37Rv identified SNPs for all genes. Almost half corresponded to not previously described SNPs and a large proportion seemed specific to Beijing/W strains. SNPs were much more informative than LSPs distinguishing twenty-four groups for which a hypothetic phylogenetic network was constructed. Phylogenetic relations originated by SNPs were found to be congruent with the ones originated by LSPs and also with previous studies on mutT2, muT4 and ogt putative DNA repair genes. Analysis according to geographic origin of isolates suggested that the collection used might be representative of the Beijing/W family. Certain genes were found to be more polymorphic than others with the accumulation of several non-synonymous SNPs (nsSNPs) that may potentially affect the function of the protein. Altered function in proteins involved in such important mechanisms in genome dynamics and stability might be associated with mutator phenotypes strains having high mutation rates in Beijing/W strains, as previously suggested. This might explain their higher adaptability and frequent association with resistance to antibiotics. Overall, this study provided useful information that, although may need to be validated using a larger set of isolates, it could be the start point for several future studies. It may contribute to clarify the role of the Beijing/W family in the tuberculosis pandemic.Resumo alargado disponível em portuguêsGicquel, BrigitteSantos, MárioRepositório da Universidade de LisboaMestre, Olga Maria Elviro, 1984-2010-07-27T08:55:22Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1188enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:31Zoai:repositorio.ul.pt:10451/1188Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:27:51.411683Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
Mycobacterium tuberculosis
title Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
spellingShingle Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
Mestre, Olga Maria Elviro, 1984-
Genética microbiana
Mycobacterium tuberculosis
Teses de Mestrado
title_short Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
title_full Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
title_fullStr Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
title_full_unstemmed Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
title_sort Genetic diversity in Mycobacterium tuberculosis: the Beijing/W family
author Mestre, Olga Maria Elviro, 1984-
author_facet Mestre, Olga Maria Elviro, 1984-
author_role author
dc.contributor.none.fl_str_mv Gicquel, Brigitte
Santos, Mário
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Mestre, Olga Maria Elviro, 1984-
dc.subject.por.fl_str_mv Genética microbiana
Mycobacterium tuberculosis
Teses de Mestrado
topic Genética microbiana
Mycobacterium tuberculosis
Teses de Mestrado
description Tese de mestrado em Biologia Molecular e Genética apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2007
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
2010-07-27T08:55:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1188
url http://hdl.handle.net/10451/1188
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
text/xml
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134161295900672

Registros relacionados