Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/90124 |
Resumo: | Cancer is one of the major health issues worldwide and further understanding of the disease is needed. To achieve this goal, new tools need to be developed that enable early diagnosis and disease prevention. To study the different stage of cancer formation, progression and invasion, different in vitro models have been developed over the years. Mostly in vitro models use 2D cultures that do not mimic the real tissue. To overcome these limitations, novel 3D tumour models need to be developed. Microfluidic devices present interesting features such as low cost, low sample and low reagent consumption, high throughput, and miniaturization, and can easily be coupled with biosensors for different applications. In this master thesis a microfluidic bioreactor was developed to enable real time monitoring of cells in a 3D tissue engineered scaffold. The micro-reactor was fabricated in Poly-methyl methacrylate (PMMA) material, due to its high strength, excellent optical properties and low cost. An electrochemical biosensor was integrated in the bioreactor to monitor the pH in the cell model, as a representation of different cancer stages. Simultaneously, the system featured an optical window to allow for inspection and fluorescence microscopy. |
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Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell culturesMicrofluidics3D cell cultureelectrochemical sensingSingle cancer cellsimmunofluorescentDomínio/Área Científica::Engenharia e Tecnologia::NanotecnologiaCancer is one of the major health issues worldwide and further understanding of the disease is needed. To achieve this goal, new tools need to be developed that enable early diagnosis and disease prevention. To study the different stage of cancer formation, progression and invasion, different in vitro models have been developed over the years. Mostly in vitro models use 2D cultures that do not mimic the real tissue. To overcome these limitations, novel 3D tumour models need to be developed. Microfluidic devices present interesting features such as low cost, low sample and low reagent consumption, high throughput, and miniaturization, and can easily be coupled with biosensors for different applications. In this master thesis a microfluidic bioreactor was developed to enable real time monitoring of cells in a 3D tissue engineered scaffold. The micro-reactor was fabricated in Poly-methyl methacrylate (PMMA) material, due to its high strength, excellent optical properties and low cost. An electrochemical biosensor was integrated in the bioreactor to monitor the pH in the cell model, as a representation of different cancer stages. Simultaneously, the system featured an optical window to allow for inspection and fluorescence microscopy.Dieguez, LorenaÁguas, HugoRUNConceição, Pedro Miguel Quintas da2019-12-20T15:57:31Z2019-1220192019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/90124enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:40:11Zoai:run.unl.pt:10362/90124Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:37:08.223632Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
title |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
spellingShingle |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures Conceição, Pedro Miguel Quintas da Microfluidics 3D cell culture electrochemical sensing Single cancer cells immunofluorescent Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
title_short |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
title_full |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
title_fullStr |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
title_full_unstemmed |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
title_sort |
Low cost rapid prototyping of a microfluidic bioreactor for electrochemical sensing of 3D cancer cell cultures |
author |
Conceição, Pedro Miguel Quintas da |
author_facet |
Conceição, Pedro Miguel Quintas da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Dieguez, Lorena Águas, Hugo RUN |
dc.contributor.author.fl_str_mv |
Conceição, Pedro Miguel Quintas da |
dc.subject.por.fl_str_mv |
Microfluidics 3D cell culture electrochemical sensing Single cancer cells immunofluorescent Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
topic |
Microfluidics 3D cell culture electrochemical sensing Single cancer cells immunofluorescent Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
description |
Cancer is one of the major health issues worldwide and further understanding of the disease is needed. To achieve this goal, new tools need to be developed that enable early diagnosis and disease prevention. To study the different stage of cancer formation, progression and invasion, different in vitro models have been developed over the years. Mostly in vitro models use 2D cultures that do not mimic the real tissue. To overcome these limitations, novel 3D tumour models need to be developed. Microfluidic devices present interesting features such as low cost, low sample and low reagent consumption, high throughput, and miniaturization, and can easily be coupled with biosensors for different applications. In this master thesis a microfluidic bioreactor was developed to enable real time monitoring of cells in a 3D tissue engineered scaffold. The micro-reactor was fabricated in Poly-methyl methacrylate (PMMA) material, due to its high strength, excellent optical properties and low cost. An electrochemical biosensor was integrated in the bioreactor to monitor the pH in the cell model, as a representation of different cancer stages. Simultaneously, the system featured an optical window to allow for inspection and fluorescence microscopy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-20T15:57:31Z 2019-12 2019 2019-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/90124 |
url |
http://hdl.handle.net/10362/90124 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137988328816640 |