Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/661 |
Resumo: | The NF-κB family member p65 is central to inflammation and immunity. The purpose of this study was to identify and characterize evolutionary conserved genes modulating p65 transcriptional activity. Using an RNAi screening approach, we identified chaperonin containing TCP1 subunit η (CCTη) as a regulator of Drosophila NF-κB proteins, Dorsal and Dorsal-related immunity factor (Dif). CCTη was also found to regulate NF-κB-driven transcription in mammalian cells, acting in a promoter-specific context, downstream of IκB kinase (IKK). CCTη knockdown repressed IκBα and CXCL2/MIP2 transcription during the early phase of NF-κB activation while impairing the termination of CCL5/RANTES and CXCL10/IP10 transcription. The latter effect was associated with increased DNA binding and reduced p65 acetylation, presumably by altering the activity of histone acetyltransferase CREB-binding protein (CBP). We identified p65 lysines (K) 122 and 123 as target residues mediating the CCTη-driven termination of NF-κB-dependent transcription. We propose that CCTη regulates NF-κB activity in a manner that resolves inflammation. |
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Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT)AcetylationAnimalsBase SequenceBlotting, WesternCells, CulturedChaperoninsDNA PrimersDrosophilaElectrophoretic Mobility Shift AssayGene Knockdown TechniquesNF-kappa BPolymerase Chain ReactionRNA InterferenceTranscription, GeneticThe NF-κB family member p65 is central to inflammation and immunity. The purpose of this study was to identify and characterize evolutionary conserved genes modulating p65 transcriptional activity. Using an RNAi screening approach, we identified chaperonin containing TCP1 subunit η (CCTη) as a regulator of Drosophila NF-κB proteins, Dorsal and Dorsal-related immunity factor (Dif). CCTη was also found to regulate NF-κB-driven transcription in mammalian cells, acting in a promoter-specific context, downstream of IκB kinase (IKK). CCTη knockdown repressed IκBα and CXCL2/MIP2 transcription during the early phase of NF-κB activation while impairing the termination of CCL5/RANTES and CXCL10/IP10 transcription. The latter effect was associated with increased DNA binding and reduced p65 acetylation, presumably by altering the activity of histone acetyltransferase CREB-binding protein (CBP). We identified p65 lysines (K) 122 and 123 as target residues mediating the CCTη-driven termination of NF-κB-dependent transcription. We propose that CCTη regulates NF-κB activity in a manner that resolves inflammation.Fundação para a Ciência e Tecnologia grants: (SFRH/BD/28016/2006, PTDC/BIA-BCM/101311/2008, PTDC/SAU-FCF/100762/2008); European Community grant: (LSH-2005-1.2.5-); National Institutes of Health grant: (HL077308).PLOSARCAPejanovic, NadjaHochrainer, KarinLiu, TaoAerne, Birgit L.Soares, Miguel P.Anrather, Josef2016-06-23T10:04:44Z2012-07-312012-07-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/661engPejanovic N, Hochrainer K, Liu T, Aerne BL, Soares MP, et al. (2012) Regulation of Nuclear Factor k B(NF- k B) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT). PLoS ONE 7(7): e42020. doi:10.1371/journal.pone.004202010.1371/journal.pone.0042020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:03Zoai:arca.igc.gulbenkian.pt:10400.7/661Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:54.068416Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
title |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
spellingShingle |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) Pejanovic, Nadja Acetylation Animals Base Sequence Blotting, Western Cells, Cultured Chaperonins DNA Primers Drosophila Electrophoretic Mobility Shift Assay Gene Knockdown Techniques NF-kappa B Polymerase Chain Reaction RNA Interference Transcription, Genetic |
title_short |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
title_full |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
title_fullStr |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
title_full_unstemmed |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
title_sort |
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT) |
author |
Pejanovic, Nadja |
author_facet |
Pejanovic, Nadja Hochrainer, Karin Liu, Tao Aerne, Birgit L. Soares, Miguel P. Anrather, Josef |
author_role |
author |
author2 |
Hochrainer, Karin Liu, Tao Aerne, Birgit L. Soares, Miguel P. Anrather, Josef |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Pejanovic, Nadja Hochrainer, Karin Liu, Tao Aerne, Birgit L. Soares, Miguel P. Anrather, Josef |
dc.subject.por.fl_str_mv |
Acetylation Animals Base Sequence Blotting, Western Cells, Cultured Chaperonins DNA Primers Drosophila Electrophoretic Mobility Shift Assay Gene Knockdown Techniques NF-kappa B Polymerase Chain Reaction RNA Interference Transcription, Genetic |
topic |
Acetylation Animals Base Sequence Blotting, Western Cells, Cultured Chaperonins DNA Primers Drosophila Electrophoretic Mobility Shift Assay Gene Knockdown Techniques NF-kappa B Polymerase Chain Reaction RNA Interference Transcription, Genetic |
description |
The NF-κB family member p65 is central to inflammation and immunity. The purpose of this study was to identify and characterize evolutionary conserved genes modulating p65 transcriptional activity. Using an RNAi screening approach, we identified chaperonin containing TCP1 subunit η (CCTη) as a regulator of Drosophila NF-κB proteins, Dorsal and Dorsal-related immunity factor (Dif). CCTη was also found to regulate NF-κB-driven transcription in mammalian cells, acting in a promoter-specific context, downstream of IκB kinase (IKK). CCTη knockdown repressed IκBα and CXCL2/MIP2 transcription during the early phase of NF-κB activation while impairing the termination of CCL5/RANTES and CXCL10/IP10 transcription. The latter effect was associated with increased DNA binding and reduced p65 acetylation, presumably by altering the activity of histone acetyltransferase CREB-binding protein (CBP). We identified p65 lysines (K) 122 and 123 as target residues mediating the CCTη-driven termination of NF-κB-dependent transcription. We propose that CCTη regulates NF-κB activity in a manner that resolves inflammation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-31 2012-07-31T00:00:00Z 2016-06-23T10:04:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/661 |
url |
http://hdl.handle.net/10400.7/661 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pejanovic N, Hochrainer K, Liu T, Aerne BL, Soares MP, et al. (2012) Regulation of Nuclear Factor k B(NF- k B) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT). PLoS ONE 7(7): e42020. doi:10.1371/journal.pone.0042020 10.1371/journal.pone.0042020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130574618624000 |